Hollow fiber supported TiO2 monolithic microextraction combined with capillary HPLC-ICP-MS for sensitive absolute quantification of phosphopeptides

2017 ◽  
Vol 32 (6) ◽  
pp. 1186-1195 ◽  
Author(s):  
Shan Li ◽  
Beibei Chen ◽  
Man He ◽  
Bin Hu
Keyword(s):  
Hollow Fiber ◽  
Absolute Quantification ◽  
Icp Ms ◽  
Quantification Analysis ◽  
Supported Tio2 ◽  
Capillary Hplc

Hollow fiber supported TiO2 monolithic microextraction combined with capHPLC-ICP-MS was proposed for absolute quantification analysis of phosphopeptides.

scholarly journals Comparison of different methods for the absolute quantification of harbour seal transferrin glycoforms using HPLC-ICP-MS

2012 ◽  
Vol 27 (3) ◽  
pp. 440 ◽  
Author(s):  
Mechthild Grebe ◽  
Daniel Pröfrock ◽  
Antje Kakuschke ◽  
M. Estella del Castillo Busto ◽  
Maria Montes-Bayón ◽  
...  
Keyword(s):  
Absolute Quantification ◽  
Harbour Seal ◽  
Icp Ms ◽  
The Absolute

scholarly journals Elevated levels of miR-483-5p in oocytes aggravates premature ovarian insufficiency induced by CDDP by targeting the FKBP4

2020 ◽  
Author(s):  
huihui zhao ◽  
Wenqing Gu ◽  
Huogui Ouyang ◽  
Wenbin Pan ◽  
Hanbin Zhang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Sex Hormones ◽  
Ovarian Function ◽  
Target Prediction ◽  
Absolute Quantification ◽  
Premature Ovarian Insufficiency ◽  
Ovarian Insufficiency ◽  
Real Time Quantitative Pcr ◽  
Quantification Analysis

Abstract Background Premature ovarian insufficiency (POI) is characterized by a loss of ovarian function before 40 years-of-age and represents a major cause of female infertility. POI is one of the dominant causes of cis-diaminedichloroplatinum (cisplatin, CDDP)-induced reproductive impairment. However, the detailed mechanisms underlying POI induced by CDDP remain unclear. Methods The POI C57B6/J mouse model was created by administering CDDP. The effects of FKBP4 were investigated using isobaric tags for relative and absolute quantification analysis (iTRAQ), real-time quantitative PCR (qRT-PCR) and western blotting. Target prediction was predicted using TargetScan software. Levels of sex hormones were tested using Enzyme-linked immunosorbent assays (ELISA). Results We found that the FKBP4 protein was down-regulated in the ovaries of CDDP model. Target prediction identified FKBP4 as a potential target for miR-483-5p , which was expressed at high levels in both the ovaries and serum of CDDP-POI mice, and in the serum from POI patients. In vitro experiments further confirmed that FKBP4 was the target for miR-483-5p in human cervical cancer cells (HeLa). The overexpression of FKBP4 in human granulosa cells (KGN) alleviated the apoptosis caused by CDDP and the overexpression of miR-483-5p . Furthermore, the overexpression of miR-483-5p in oocytes caused injury to the ovaries, and disrupted the levels of sex hormones in CDDP-POI mice (AMH: P <0.01; E 2 : P <0.01; FSH: P <0.01). Conclusions Analyses showed that miR-483-5p targets the FKBP4 protein in a mouse model of POI induced by CDDP. Elevated levels of miR-483-5p in oocytes aggravated POI induced by CDDP by targeting FKBP4. Overall, our data demonstrate that miR-483-5p was responsible for the underlying pathophysiology of POI induced by chemotherapeutic treatments, such as CDDP.

scholarly journals Elevated levels of miR-483-5p in oocytes aggravates premature ovarian insufficiency induced by CDDP by targeting the FKBP4

2020 ◽  
Author(s):  
huihui zhao ◽  
Wenqing Gu ◽  
Huogui Ouyang ◽  
Wenbin Pan ◽  
Hanbin Zhang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Sex Hormones ◽  
Ovarian Function ◽  
Target Prediction ◽  
Absolute Quantification ◽  
Premature Ovarian Insufficiency ◽  
Ovarian Insufficiency ◽  
Real Time Quantitative Pcr ◽  
Quantification Analysis

Abstract Background: Premature ovarian insufficiency (POI) is characterized by a loss of ovarian function before 40 years-of-age and represents an existing challenge cause of female infertility. POI is one of the dominant causes of cis-diaminedichloroplatinum (cisplatin, CDDP)-induced reproductive impairment. However, the detailed mechanisms underlying POI induced by CDDP remain unclear.Methods: The POI C57B6/J mouse model was created by administering CDDP. The effects of FKBP4 were investigated using isobaric tags for relative and absolute quantification analysis (iTRAQ), real-time quantitative PCR (qRT-PCR) and western blotting. Target prediction was predicted using TargetScan software. Levels of sex hormones were tested using Enzyme-linked immunosorbent assays (ELISA).Results: We found that the FKBP4 protein was down-regulated in the ovaries of CDDP model. Target prediction identified FKBP4 as a potential target for miR-483-5p, which was expressed at high levels in both the ovaries and serum of CDDP-POI mice, and in the serum from POI patients. In vitro experiments further confirmed that FKBP4 was the target for miR-483-5p in human cervical cancer cells (HeLa). The overexpression of FKBP4 in human granulosa cells (KGN) alleviated the apoptosis caused by CDDP and the overexpression of miR-483-5p. Furthermore, the overexpression of miR-483-5p in oocytes caused injury to the ovaries, and disrupted the levels of sex hormones in CDDP-POI mice (AMH: P < 0.01; E2: P < 0.01; FSH: P < 0.01).Conclusions: Analyses showed that miR-483-5p targets the FKBP4 protein in a mouse model of POI induced by CDDP. Elevated levels of miR-483-5p in oocytes could aggravate POI induced by CDDP by targeting FKBP4. Overall, our data demonstrate that miR-483-5p was responsible for the underlying pathophysiology of POI induced by chemotherapeutic treatments, such as CDDP.

scholarly journals Absolute quantification of transferrin in blood samples of harbour seals using HPLC-ICP-MS

Metallomics ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 176 ◽  
Author(s):  
Mechthild Grebe ◽  
Daniel Pröfrock ◽  
Antje Kakuschke ◽  
Jose A. C. Broekaert ◽  
Andreas Prange
Keyword(s):  
Absolute Quantification ◽  
Blood Samples ◽  
Harbour Seals ◽  
Icp Ms

Capillary HPLC−ICPMS and Tyrosine Iodination for the Absolute Quantification of Peptides Using Generic Standards

2009 ◽  
Vol 81 (13) ◽  
pp. 5390-5399 ◽  
Author(s):  
Ana Pereira Navaza ◽  
Jorge Ruiz Encinar ◽  
Alfredo Ballesteros ◽  
José M. González ◽  
Alfredo Sanz-Medel
Keyword(s):  
Absolute Quantification ◽  
The Absolute ◽  
Capillary Hplc
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