Biosynthesis, purification and analysis of selenomethionyl calmodulin by gel electrophoresis-laser ablation-ICP-MS and capillary HPLC-ICP-MS peptide mapping following in-gel tryptic digestion

2005 ◽  
Vol 20 (6) ◽  
pp. 493 ◽  
Author(s):  
Guillaume Ballihaut ◽  
Laure Tastet ◽  
Christophe Pécheyran ◽  
Brice Bouyssiere ◽  
Olivier Donard ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Gel Electrophoresis ◽  
Peptide Mapping ◽  
Tryptic Digestion ◽  
Icp Ms ◽  
Capillary Hplc

Detection of selenoproteins in human cell extracts by laser ablation-ICP MS after separation by polyacrylamide gel electrophoresis and blotting

2012 ◽  
Vol 27 (1) ◽  
pp. 25-32 ◽  
Author(s):  
Juliusz Bianga ◽  
Guillaume Ballihaut ◽  
Christophe Pécheyran ◽  
Zahia Touat ◽  
Hugues Preud'homme ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Human Cell ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Cell Extracts ◽  
Icp Ms

Imaging of metals and metal-containing species in biological tissues and on gels by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS): A new analytical strategy for applications in life sciences

2008 ◽  
Vol 80 (12) ◽  
pp. 2643-2655 ◽  
Author(s):  
J. Sabine Becker ◽  
M. Zoriy ◽  
Valderi L. Dressler ◽  
Bei Wu ◽  
J. Susanne Becker
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Gel Electrophoresis ◽  
Inductively Coupled Plasma ◽  
Toxic Elements ◽  
Life Sciences ◽  
Biological Tissues ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has become established as a very efficient and sensitive trace, ultratrace, and surface analytical technique in the life sciences. We have developed a new analytical imaging technique using LA-ICP-MS to study element distribution in biological tissues. Nowadays, LA imaging ICP-MS using double-focusing sector field (LA-ICP-SFMS) or quadrupole-based mass spectrometers (LA-ICP-QMS) can be applied as an exciting tool providing new information on the pathophysiology, pharmacology, and toxicology of elements of interest in biological systems. The quantitative determination of elements (e.g., Cu, Fe, Zn, Se, and others) in biological tissues is of growing interest especially in brain research (e.g., for studying neurodegenerative diseases such as Alzheimer's or Parkinson's disease). LA-ICP-SFMS was employed to produce images of detailed regionally specific element distributions in thin tissue sections of different sizes (such as control human or rat brain tissues or tumor regions). In addition, imaging MS using LA-ICP-QMS was applied to study the uptake and transport of nutrient and toxic elements in plant tissues.Besides the quantitative imaging of essential and toxic elements in tissues, powerful analytical techniques are also required for the determination and characterization of phosphoproteins and metal-containing proteins within a large pool of proteins, after electrophoretic separation (e.g., blue native, BN and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, SDS-PAGE) into 1D and 2D gels. LA-ICP-MS was used to detect metalloproteins in protein bands of 1D gels or protein spots separated after 2D gel electrophoresis (2D-GE). In addition to elemental determination by LA-ICP-MS, matrix-assisted laser desorption/ionization (MALDI)-MS was employed to identify metal-containing proteins. Recent progress will be discussed in applying LA-ICP-MS in the life sciences, including the imaging of thin slices of tissue and applications in proteome analysis in combination with MALDI-MS to investigate phosphoproteins and metal-containing proteins.

Evaluation of gel electrophoresis conditions for the separation of metal-tagged proteins with subsequent laser ablation ICP-MS detection

2009 ◽  
Vol 30 (2) ◽  
pp. 303-314 ◽  
Author(s):  
Andrea Raab ◽  
Barbara Pioselli ◽  
Caroline Munro ◽  
Jane Thomas-Oates ◽  
Jörg Feldmann
Keyword(s):  
Laser Ablation ◽  
Gel Electrophoresis ◽  
Ms Detection ◽  
Icp Ms

Detection of Zn-containing proteins in slug (Genus Arion) tissue using laser ablation ICP-MS after separation by gel electrophoresis

2011 ◽  
Vol 307 (1-3) ◽  
pp. 66-69 ◽  
Author(s):  
J. Susanne Becker ◽  
Dirce Pozebon ◽  
Andreas Matusch ◽  
Valderi L. Dressler ◽  
J. Sabine Becker
Keyword(s):  
Laser Ablation ◽  
Gel Electrophoresis ◽  
Icp Ms

Speciation of iodine-containing proteins in Nori seaweed by gel electrophoresis laser ablation ICP–MS

Talanta ◽  
2014 ◽  
Vol 127 ◽  
pp. 175-180 ◽  
Author(s):  
V. Romarís-Hortas ◽  
J. Bianga ◽  
A. Moreda-Piñeiro ◽  
P. Bermejo-Barrera ◽  
J. Szpunar
Keyword(s):  
Laser Ablation ◽  
Gel Electrophoresis ◽  
Icp Ms

Speciation of selenoproteins in Se-contaminated wildlife by gel electrophoresis and laser ablation-ICP-MS

2002 ◽  
Vol 17 (12) ◽  
pp. 1621-1623 ◽  
Author(s):  
Teresa W.-M. Fan ◽  
Ewa Pruszkowski ◽  
Stephen Shuttleworth
Keyword(s):  
Laser Ablation ◽  
Gel Electrophoresis ◽  
Icp Ms
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