Polydatin inhibits the IL-1β-induced inflammatory response in human osteoarthritic chondrocytes by activating the Nrf2 signaling pathway and ameliorates murine osteoarthritis

2018 ◽  
Vol 9 (3) ◽  
pp. 1701-1712 ◽  
Author(s):  
Shangkun Tang ◽  
Qian Tang ◽  
Jialei Jin ◽  
Gang Zheng ◽  
Jianchen Xu ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Inflammatory Response ◽  
Signaling Pathway ◽  
Osteoarthritic Chondrocytes ◽  
Nrf2 Signaling Pathway ◽  
Prevalent Form

Osteoarthritis (OA), which is characterized by progressive degradation of the articular cartilage, is the most prevalent form of human arthritis.

scholarly journals Anti-Inflammatory Effects of Neochlorogenic Acid Extract from Mulberry Leaf (Morus alba L.) Against LPS-Stimulated Inflammatory Response through Mediating the AMPK/Nrf2 Signaling Pathway in A549 Cells

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1385 ◽  
Author(s):  
Xiao-han Gao ◽  
Sun-dong Zhang ◽  
Li-tao Wang ◽  
Liang Yu ◽  
Xue-lian Zhao ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Signaling Pathway ◽  
A549 Cells ◽  
Morus Alba ◽  
Mulberry Leaf ◽  
Tnf Α ◽  
Nrf2 Signaling Pathway ◽  
Acute Pneumonia ◽  
Neochlorogenic Acid ◽  
Anti Inflammatory

Neochlorogenic acid (nCGA) is a phenolic compound isolated from mulberry leaf (Morus alba L.), which possesses multiple pharmacological activities containing antioxidant and anti-inflammatory effects. However, the role of nCGA in the treatment of acute pneumonia and the underlying molecular mechanism are still unclear. Hence, the aim of study is to investigate the anti-inflammatory properties of nCGA on LPS-stimulated inflammation in A549 cells. In the present study, results reported that nCGA without cytotoxicity significantly reduced the production of TNF-α, IL-6, and NO, and further suppressed the proteins of iNOS, COX2, TNF-α, IL-6 expression. Furthermore, nCGA also inhibited NF-κB activation and blocked MAPKs signaling pathway phosphorylation. In addition, we found nCGA significantly increased the expression of HO-1 via activating the AMPK/Nrf2 signaling pathway to attenuate the inflammatory response, whereas this protective effect of nCGA was reversed by pre-treatment with compound C (C.C, an AMPK inhibitor). Therefore, all these results indicated that nCGA might act as a natural anti-inflammatory agent for the treatment of acute pneumonia.

Overexpression of immune-responsive gene 1 prevents acute otitis media due to streptococcus pneumoniae infection through Nrf2- Hmox1 pathway

2019 ◽  
Author(s):  
Peizhen Yang ◽  
Yufen Li ◽  
Dongqing Wang ◽  
Zhipeng Chen
Keyword(s):  
Streptococcus Pneumoniae ◽  
Inflammatory Response ◽  
Signaling Pathway ◽  
Otitis Media ◽  
Middle Ear ◽  
Acute Otitis Media ◽  
Lavage Fluid ◽  
Mice Model ◽  
Nrf2 Signaling Pathway ◽  
Positive Rate

Abstract Background: Acute otitis media (AOM) is one of the most commonly diagnosed childhood diseases that are associated with influenza infection. Immunoresponsive gene 1 (Irg1) is overexpressed in mammalian macrophage during inflammation process. Here, the present study aims to investigate the role of Irg1 in streptococcus pneumoniae infection-induced inflammatory response in AOM through Nrf2 signaling pathway. Methods: Positive rate of streptococcus pneumoniae and expression of Irg1, Nrf2 and Hmox1 were determined in the middle ear lavage fluid from 85 AOM patients infected with streptococcus pneumoniae and 48 healthy subjects. After the successful establishment of mice model with AOM, macrophage was harvest from the middle ear lavage fluid of mice. At last, to investigate the effect of Irg1 and Nrf2 signaling pathway on inflammatory response and streptococcus pneumoniae infection by accumulating macrophage, Irg1, sh-Irg1 and retinoic acid (an inhibitor of Nrf2 signaling pathway) were injected into AOM mice. Results: Irg1 exhibited a high level and activated Nrf2 signaling pathway was detected in AOM. Besides, positive rate of streptococcus pneumoniae was increased in AOM. Furthermore, in the mice model with AOM, Irg1 could repress inflammatory response by downregulating expression of TNF-α, IL-6 and IL-1β and inhibit streptococcus pneumoniae infection by stimulating accumulation of macrophage to increase its endocytosis through activation of Nrf2 signaling pathway. Conclusion: Taken together, Irg1 contributes to inhibiting inflammatory response and streptococcus pneumoniae infection in AOM by promoting accumulation of macrophage through activation of Nrf2 signaling pathway, which provides novel therapeutic targets for AOM therapy.

scholarly journals Sesamin inhibits IL-1β-stimulated inflammatory response in human osteoarthritis chondrocytes by activating Nrf2 signaling pathway

Oncotarget ◽  
2016 ◽  
Vol 7 (50) ◽  
pp. 83720-83726 ◽  
Author(s):  
Pengyu Kong ◽  
Guanghua Chen ◽  
Anlong Jiang ◽  
Yufu Wang ◽  
Chengchao Song ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Signaling Pathway ◽  
Nrf2 Signaling Pathway ◽  
Osteoarthritis Chondrocytes
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