Characterizing protein–protein-interaction in high-concentration monoclonal antibody systems with the quartz crystal microbalance

2017 ◽  
Vol 19 (48) ◽  
pp. 32698-32707 ◽  
Author(s):  
Josef Hartl ◽  
Astrid Peschel ◽  
Diethelm Johannsmann ◽  
Patrick Garidel
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Protein Interaction ◽  
Quartz Crystal ◽  
Therapeutic Antibodies ◽  
High Concentration ◽  
Concentrated Solutions ◽  
Protein Protein Interaction

Making use of a quartz crystal microbalance, concentrated solutions of therapeutic antibodies were studied with respect to their behavior under shear excitation with frequencies in the MHz range.

Real-time heterogeneous protein–protein interaction between αA-crystallin N-terminal mutants and αB-crystallin using quartz crystal microbalance (QCM)

Amino Acids ◽  
2015 ◽  
Vol 47 (5) ◽  
pp. 1035-1043 ◽  
Author(s):  
Srinivasagan Ramkumar ◽  
Noriko Fujii ◽  
Hiroaki Sakaue ◽  
Norihiko Fujii ◽  
Bency Thankappan ◽  
...  
Keyword(s):  
Real Time ◽  
Quartz Crystal Microbalance ◽  
Protein Interaction ◽  
Quartz Crystal ◽  
Protein Protein Interaction ◽  
Αb Crystallin

scholarly journals Quantitative Assessment of Periodontal Bacteria Using a Cell-Based Immunoassay with Functionalized Quartz Crystal Microbalance

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 159
Author(s):  
Satit Rodphukdeekul ◽  
Miyuki Tabata ◽  
Chindanai Ratanaporncharoen ◽  
Yasuo Takeuchi ◽  
Pakpum Somboon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Quantitative Assessment ◽  
Quartz Crystal ◽  
Dynamic Range ◽  
Gold Surface ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Disorder ◽  
Label Free ◽  
Periodontal Bacteria

Periodontal disease is an inflammatory disorder that is triggered by bacterial plaque and causes the destruction of the tooth-supporting tissues leading to tooth loss. Several bacteria species, including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, are considered to be associated with severe periodontal conditions. In this study, we demonstrated a quartz crystal microbalance (QCM) immunoassay for quantitative assessment of the periodontal bacteria, A. actinomycetemcomitans. An immunosensor was constructed using a self-assembled monolayer of 11-mercaptoundecanoic acid (11-MUA) on the gold surface of a QCM chip. The 11-MUA layer was evaluated using a cyclic voltammetry technique to determine its mass and packing density. Next, a monoclonal antibody was covalently linked to 11-MUA using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to act as the biorecognition element. The specificity of the monoclonal antibody was confirmed by an enzyme-linked immunosorbent assay. A calibration curve, for the relationship between the frequency shifts and number of bacteria, was used to calculate the number of A. actinomycetemcomitans bacteria in a test sample. Based on a regression equation, the lower detection limit was 800 cells, with a dynamic range up to 2.32 × 106 cells. Thus, the QCM biosensor in this study provides a sensitive and label-free method for quantitative analysis of periodontal bacteria. The method can be used in various biosensing assays for practical application and routine detection of periodontitis pathogens.

Quartz Crystal Microbalance Coated with 18-Crown-6 Ether Film for 2,4,6-Trinitrotolurene (TNT) Vapor Detection

2017 ◽  
Vol 748 ◽  
pp. 358-362
Author(s):  
Pavaris Phuvanatai ◽  
Toempong Phetchakul
Keyword(s):  
Steady State ◽  
Frequency Shift ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Film Surface ◽  
Vapor Concentration ◽  
High Concentration ◽  
Vapor Detection ◽  
Steady State Condition ◽  
Shift Response

The 2, 4, 6-trinitrotoluene (TNT) vapor detection by quartz crystal microbalance (QCM) coated with 18-crown-6 ether film is introduced. The film acts as selective or sensitive layer for detecting TNT molecule. The frequency shift response is depend on the amount of mass of molecule adhere on the coated film surface. In this study, the concentrations of TNT vapor which is related to responses are studied. It shows that the TNT vapor concentration is related to the time for steady state condition and the frequency shift response. The high concentration vapor will increase the frequency shift response but increase the steady state time.

scholarly journals Photo-Click Immobilization on Quartz Crystal Microbalance Sensors for Selective Carbohydrate−Protein Interaction Analyses

2011 ◽  
Vol 83 (3) ◽  
pp. 1000-1007 ◽  
Author(s):  
Oscar Norberg ◽  
Lingquan Deng ◽  
Teodor Aastrup ◽  
Mingdi Yan ◽  
Olof Ramström
Keyword(s):  
Quartz Crystal Microbalance ◽  
Protein Interaction ◽  
Quartz Crystal
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