Immunophenotyping of Acute Leukemias Using a Quartz Crystal Microbalance and Monoclonal Antibody-Coated Magnetic Microspheres

2006 ◽  
Vol 78 (8) ◽  
pp. 2571-2578 ◽  
Author(s):  
Hua Wang ◽  
Hui Zeng ◽  
Guoli Shen ◽  
Ruqin Yu
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Magnetic Microspheres ◽  
Acute Leukemias

scholarly journals Quantitative Assessment of Periodontal Bacteria Using a Cell-Based Immunoassay with Functionalized Quartz Crystal Microbalance

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 159
Author(s):  
Satit Rodphukdeekul ◽  
Miyuki Tabata ◽  
Chindanai Ratanaporncharoen ◽  
Yasuo Takeuchi ◽  
Pakpum Somboon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Quantitative Assessment ◽  
Quartz Crystal ◽  
Dynamic Range ◽  
Gold Surface ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Disorder ◽  
Label Free ◽  
Periodontal Bacteria

Periodontal disease is an inflammatory disorder that is triggered by bacterial plaque and causes the destruction of the tooth-supporting tissues leading to tooth loss. Several bacteria species, including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, are considered to be associated with severe periodontal conditions. In this study, we demonstrated a quartz crystal microbalance (QCM) immunoassay for quantitative assessment of the periodontal bacteria, A. actinomycetemcomitans. An immunosensor was constructed using a self-assembled monolayer of 11-mercaptoundecanoic acid (11-MUA) on the gold surface of a QCM chip. The 11-MUA layer was evaluated using a cyclic voltammetry technique to determine its mass and packing density. Next, a monoclonal antibody was covalently linked to 11-MUA using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to act as the biorecognition element. The specificity of the monoclonal antibody was confirmed by an enzyme-linked immunosorbent assay. A calibration curve, for the relationship between the frequency shifts and number of bacteria, was used to calculate the number of A. actinomycetemcomitans bacteria in a test sample. Based on a regression equation, the lower detection limit was 800 cells, with a dynamic range up to 2.32 × 106 cells. Thus, the QCM biosensor in this study provides a sensitive and label-free method for quantitative analysis of periodontal bacteria. The method can be used in various biosensing assays for practical application and routine detection of periodontitis pathogens.

Characterizing protein–protein-interaction in high-concentration monoclonal antibody systems with the quartz crystal microbalance

2017 ◽  
Vol 19 (48) ◽  
pp. 32698-32707 ◽  
Author(s):  
Josef Hartl ◽  
Astrid Peschel ◽  
Diethelm Johannsmann ◽  
Patrick Garidel
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Protein Interaction ◽  
Quartz Crystal ◽  
Therapeutic Antibodies ◽  
High Concentration ◽  
Concentrated Solutions ◽  
Protein Protein Interaction

Making use of a quartz crystal microbalance, concentrated solutions of therapeutic antibodies were studied with respect to their behavior under shear excitation with frequencies in the MHz range.

Development of quartz-crystal-microbalance-based immunosensor array for clinical immunophenotyping of acute leukemias

2006 ◽  
Vol 351 (1) ◽  
pp. 69-76 ◽  
Author(s):  
Hui Zeng ◽  
Hua Wang ◽  
Fangping Chen ◽  
Hongya Xin ◽  
Guangping Wang ◽  
...  
Keyword(s):  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Acute Leukemias

Interaction of Clear Flavor Emulsions Containing Lemon Essential Oils with Lipid Bilayers via a Quartz Crystal Microbalance

2019 ◽  
Vol 25 (6) ◽  
pp. 879-884
Author(s):  
Takahiro Sakai ◽  
Hayato Seki ◽  
Shogo Yoshida ◽  
Hayato Hori ◽  
Hisashi Suzuki ◽  
...  
Keyword(s):  
Essential Oils ◽  
Lipid Bilayers ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal
Sign in / Sign up

Export Citation Format

Share Document