SypHer3s: a genetically encoded fluorescent ratiometric probe with enhanced brightness and an improved dynamic range

2018 ◽  
Vol 54 (23) ◽  
pp. 2898-2901 ◽  
Author(s):  
Y. G. Ermakova ◽  
V. V. Pak ◽  
Y. A. Bogdanova ◽  
A. A. Kotlobay ◽  
I. V. Yampolsky ◽  
...  

We designed a genetically encoded ratiometric fluorescent probe, SypHer3s, with enhanced brightness and optimized pKa, which responds to pH changes in different cellular compartments.

2017 ◽  
Vol 5 (2) ◽  
pp. 289-295 ◽  
Author(s):  
Shi-Li Shen ◽  
Xuan Zhao ◽  
Xiao-Fan Zhang ◽  
Xuan-Li Liu ◽  
Hao Wang ◽  
...  

A novel ratiometric probe (RCP) for −OCl was developed based on the fluorescence resonance energy transfer (FRET) platform.


2022 ◽  
Author(s):  
Wenjie Liu ◽  
Dandan Bu ◽  
Hongyan Zhang ◽  
Meng Zhang ◽  
Haohui Ren ◽  
...  

Based on coumarin and benzopyran derivatives, a dual-wavelength excitation ratiometric fluorescent probe HABA was prepared to detect H2S. The HABA showed good selectivity and anti-interference to H2S. Fluorescence co-localization experiments...


2018 ◽  
Vol 6 (36) ◽  
pp. 9636-9641 ◽  
Author(s):  
Zhi Shen ◽  
Cheng Zhang ◽  
Xinling Yu ◽  
Jian Li ◽  
Zhenyang Wang ◽  
...  

Fluorescent carbon dots were synthesized by microwave irradiation of cyclen and citric acid to construct a ratiometric probe for tetracycline detection.


2018 ◽  
Vol 6 (13) ◽  
pp. 1973-1983 ◽  
Author(s):  
Yong Liu ◽  
Jing Nie ◽  
Jie Niu ◽  
Weishan Wang ◽  
Weiying Lin

We have developed a novel ratiometric probe, TPE-TE, monitoring SO2 with distinct ratiometric fluorescence signals in mammalian cells, mouse embryonic fibroblast, and zebrafish.


The Analyst ◽  
2015 ◽  
Vol 140 (16) ◽  
pp. 5563-5569 ◽  
Author(s):  
Qianqian Wang ◽  
Liyi Zhou ◽  
Liping Qiu ◽  
Danqing Lu ◽  
Yongxiang Wu ◽  
...  

We present a new ESIPT-based ratiometric fluorescent probeFR-Lysfor the dynamic imaging of pH changes in lysosomes.


2017 ◽  
Vol 41 (12) ◽  
pp. 5055-5060 ◽  
Author(s):  
Xiaofeng Yang ◽  
Yexin Li ◽  
Zongshi Zhao ◽  
Yiming Ding ◽  
Yan Zhang ◽  
...  

A diketopyrrolopyrrole-based fluorescent probe 1 was explored as a ratiometric probe for selective detection of Au3+, with the detection limit of 18 nM.


RSC Advances ◽  
2017 ◽  
Vol 7 (87) ◽  
pp. 55296-55300 ◽  
Author(s):  
Shi-Li Shen ◽  
Xiao-Fan Zhang ◽  
Yan-Qing Ge ◽  
Yan Zhu ◽  
Xiao-Qun Cao

A novel mitochondria-targeting ratiometric probe (IRP) for −OCl based on an imidazo[1,5-a]pyridine-rhodamine FRET platform was developed.


Talanta ◽  
2019 ◽  
Vol 192 ◽  
pp. 6-13 ◽  
Author(s):  
Zhen Liu ◽  
Guoping Li ◽  
Yana Wang ◽  
Jiulong Li ◽  
Yang Mi ◽  
...  

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