Modular design of optically controlled protein affinity reagents

2018 ◽  
Vol 54 (13) ◽  
pp. 1591-1594 ◽  
Author(s):  
A. Babalhavaeji ◽  
G. A. Woolley
Keyword(s):  
Modular Design ◽  
Optical Control ◽  
Affinity Reagents ◽  
Protein Affinity ◽  
Affinity Reagent

Reversible, optical control of a generalizable protein affinity reagent.

scholarly journals Creating Protein Affinity Reagents by Combining Peptide Ligands on Synthetic DNA Scaffolds

2009 ◽  
Vol 131 (47) ◽  
pp. 17233-17241 ◽  
Author(s):  
Berea A. R. Williams ◽  
Chris W. Diehnelt ◽  
Paul Belcher ◽  
Matthew Greving ◽  
Neal W. Woodbury ◽  
...  
Keyword(s):  
Peptide Ligands ◽  
Affinity Reagents ◽  
Synthetic Dna ◽  
Protein Affinity ◽  
Dna Scaffolds

scholarly journals A Community Standard Format for the Representation of Protein Affinity Reagents

2009 ◽  
Vol 9 (1) ◽  
pp. 1-10 ◽  
Author(s):  
David E. Gloriam ◽  
Sandra Orchard ◽  
Daniela Bertinetti ◽  
Erik Björling ◽  
Erik Bongcam-Rudloff ◽  
...  
Keyword(s):  
Standard Format ◽  
Affinity Reagents ◽  
Protein Affinity ◽  
Community Standard

scholarly journals The NIH Protein Capture Reagents Program (PCRP): a standardized protein affinity reagent toolbox

2016 ◽  
Vol 13 (10) ◽  
pp. 805-806 ◽  
Author(s):  
Seth Blackshaw ◽  
Anand Venkataraman ◽  
Jose Irizarry ◽  
Kun Yang ◽  
Stephen Anderson ◽  
...  
Keyword(s):  
Protein Capture ◽  
Protein Affinity ◽  
Affinity Reagent

scholarly journals Photocontrol of Protein Affinity Reagents by Red Light using Azobenzene Switches

2016 ◽  
Vol 110 (3) ◽  
pp. 539a
Author(s):  
Amirhossein Babalhavaeji ◽  
Mingxin Dong ◽  
Di Wu ◽  
G. Andrew Woolley
Keyword(s):  
Red Light ◽  
Affinity Reagents ◽  
Protein Affinity

Abiotic Stimuli-Responsive Protein Affinity Reagent for IgG

2021 ◽  
Author(s):  
Shunsuke Onogi ◽  
Shih-Hui Lee ◽  
Krista R. Fruehauf ◽  
Kenneth J. Shea
Keyword(s):  
Stimuli Responsive ◽  
Protein Affinity ◽  
Affinity Reagent ◽  
Abiotic Stimuli

scholarly journals Minimum information about a protein affinity reagent (MIAPAR)

2010 ◽  
Vol 28 (7) ◽  
pp. 650-653 ◽  
Author(s):  
Julie Bourbeillon ◽  
Sandra Orchard ◽  
Itai Benhar ◽  
Carl Borrebaeck ◽  
Antoine de Daruvar ◽  
...  
Keyword(s):  
Minimum Information ◽  
Protein Affinity ◽  
Affinity Reagent

Modular design

1999 ◽  
Vol 78 (3) ◽  
pp. 113-116 ◽  
Keyword(s):  
Modular Design

On-site optical control for solar mirrors

2014 ◽  
Author(s):  
D. Fontani ◽  
P. Sansoni ◽  
S. Coraggia ◽  
L. Mercatelli ◽  
D. Jafrancesco ◽  
...  
Keyword(s):  
Optical Control

PHOTACs Enable Optical Control of Protein Degradation

2019 ◽  
Author(s):  
Martin Reynders ◽  
Bryan Matsuura ◽  
Marleen Bérouti ◽  
Daniele Simoneschi ◽  
Antonio Marzio ◽  
...  
Keyword(s):  
Protein Degradation ◽  
E3 Ligase ◽  
Optical Control ◽  
Cellular Proteins ◽  
Bifunctional Molecules ◽  
Protein Levels ◽  
Lead Compounds ◽  
Subsequent Degradation ◽  
Temporal And Spatial ◽  
Precision Therapeutics

<p><i>PROTACs (proteolysis targeting chimeras) are bifunctional molecules that tag proteins for ubiquitylation by an E3 ligase complex and subsequent degradation by the proteasome. They have emerged as powerful tools to control the levels of specific cellular proteins and are on the verge of being clinically used. We now introduce photoswitchable PROTACs that can be activated with the temporal and spatial precision that light provides. These trifunctional molecules, which we named PHOTACs, consist of a ligand for an E3 ligase, a photoswitch, and a ligand for a protein of interest. We demonstrate this concept by using PHOTACs that target either BET family proteins (BRD2,3,4) or FKBP12. Our lead compounds display little or no activity in the dark but can be reversibly activated to varying degrees with different wavelengths of light. Our modular and generalizable approach provides a method for the optical control of protein levels with photopharmacology and could lead to new types of precision therapeutics that avoid undesired systemic toxicity.</i><b></b></p>

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