scholarly journals Creating Protein Affinity Reagents by Combining Peptide Ligands on Synthetic DNA Scaffolds

2009 ◽  
Vol 131 (47) ◽  
pp. 17233-17241 ◽  
Author(s):  
Berea A. R. Williams ◽  
Chris W. Diehnelt ◽  
Paul Belcher ◽  
Matthew Greving ◽  
Neal W. Woodbury ◽  
...  
Keyword(s):  
Peptide Ligands ◽  
Affinity Reagents ◽  
Synthetic Dna ◽  
Protein Affinity ◽  
Dna Scaffolds

scholarly journals A Community Standard Format for the Representation of Protein Affinity Reagents

2009 ◽  
Vol 9 (1) ◽  
pp. 1-10 ◽  
Author(s):  
David E. Gloriam ◽  
Sandra Orchard ◽  
Daniela Bertinetti ◽  
Erik Björling ◽  
Erik Bongcam-Rudloff ◽  
...  
Keyword(s):  
Standard Format ◽  
Affinity Reagents ◽  
Protein Affinity ◽  
Community Standard

A phage display-based strategy for the de novo creation of disulfide-constrained and isomer-free bicyclic peptide affinity reagents

2018 ◽  
Vol 54 (32) ◽  
pp. 4029-4032 ◽  
Author(s):  
Mirao Zha ◽  
Ping Lin ◽  
Hongwei Yao ◽  
Yibing Zhao ◽  
Chuanliu Wu
Keyword(s):  
Phage Display ◽  
Genetic Code ◽  
De Novo ◽  
Screening Strategy ◽  
Peptide Ligands ◽  
Disulfide Bridges ◽  
Chemical Modifications ◽  
Affinity Reagents ◽  
Peptide Affinity

We report a phage-screening strategy for the development of bicyclic peptide ligands constrained with two sterically different and isomerically forbidden noncanonical disulfide bridges without elaborate chemical modifications and recourses to genetic code reprogramming.

scholarly journals Photocontrol of Protein Affinity Reagents by Red Light using Azobenzene Switches

2016 ◽  
Vol 110 (3) ◽  
pp. 539a
Author(s):  
Amirhossein Babalhavaeji ◽  
Mingxin Dong ◽  
Di Wu ◽  
G. Andrew Woolley
Keyword(s):  
Red Light ◽  
Affinity Reagents ◽  
Protein Affinity

scholarly journals Engineering couplings for exciton transport using synthetic DNA scaffolds

Chem ◽  
2021 ◽  
Author(s):  
Stephanie M. Hart ◽  
Wei Jia Chen ◽  
James L. Banal ◽  
William P. Bricker ◽  
Amro Dodin ◽  
...  
Keyword(s):  
Synthetic Dna ◽  
Exciton Transport ◽  
Dna Scaffolds

scholarly journals Engineering couplings for exciton transport using synthetic DNA scaffolds

Chem ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 827
Author(s):  
Stephanie M. Hart ◽  
Wei Jia Chen ◽  
James L. Banal ◽  
William P. Bricker ◽  
Amro Dodin ◽  
...  
Keyword(s):  
Synthetic Dna ◽  
Exciton Transport ◽  
Dna Scaffolds

Modular design of optically controlled protein affinity reagents

2018 ◽  
Vol 54 (13) ◽  
pp. 1591-1594 ◽  
Author(s):  
A. Babalhavaeji ◽  
G. A. Woolley
Keyword(s):  
Modular Design ◽  
Optical Control ◽  
Affinity Reagents ◽  
Protein Affinity ◽  
Affinity Reagent

Reversible, optical control of a generalizable protein affinity reagent.

Ligand Design for Specific MHC Class I Molecules on the Cell Surface

2020 ◽  
Author(s):  
Xizheng Sun ◽  
Reika Tokunaga ◽  
Yoko Nagai ◽  
Ryo Miyahara ◽  
Akihiro Kishimura ◽  
...  
Keyword(s):  
Cell Surface ◽  
Mhc Class I ◽  
Targeted Delivery ◽  
Peptide Ligands ◽  
Class I ◽  
Class I Mhc ◽  
Mhc I ◽  
Histocompatibility Complex ◽  
High Binding Affinity ◽  
Mhc Class I Molecules

<p><a></a><a></a><a>We have validated that ligand peptides designed from antigen peptides could be used for targeting specific major histocompatibility complex class I (MHC-I)</a> molecules on cell surface. To design the ligand peptides, we used reported antigen peptides for each MHC-I molecule with high binding affinity. From the crystal structure of the peptide/MHC-I complexes, we determined a modifiable residue in the antigen peptides and replaced this residue with a lysine with an ε-amine group modified with functional molecules. The designed ligand peptides successfully bound to cells expressing the corresponding MHC-I molecules via exchange of peptides bound to the MHC-I. We demonstrated that the peptide ligands could be used to transport a protein or a liposome to cells expressing the corresponding MHC-I. The present strategy may be useful for targeted delivery to cells overexpressing MHC-I, which have been observed autoimmune diseases.</p>

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