scholarly journals Sensitive and Specific Detection ofMycoplasma speciesby Consensus Polymerase Chain Reaction and Dot Blot Hybridization

2011 ◽  
Vol 27 (2) ◽  
pp. 141 ◽  
Author(s):  
Sunhwa Hong ◽  
Hyun-A Lee ◽  
Sang-Ho Park ◽  
Okjin Kim
Keyword(s):  
Polymerase Chain Reaction ◽  
Blot Hybridization ◽  
Dot Blot ◽  
Specific Detection ◽  
Chain Reaction ◽  
Dot Blot Hybridization ◽  
Polymerase Chain

scholarly journals Comparative Infection Progress Analysis of Lettuce big-vein virus and Mirafiori lettuce virus in Lettuce Crops by Developed Molecular Diagnosis Techniques

2004 ◽  
Vol 94 (5) ◽  
pp. 470-477 ◽  
Author(s):  
Jose A. Navarro ◽  
Francisco Botella ◽  
Antonio Maruhenda ◽  
Pedro Sastre ◽  
M. Amelia Sánchez-Pina ◽  
...  
Keyword(s):  
Blot Hybridization ◽  
Hybridization Technique ◽  
Dot Blot ◽  
Specific Detection ◽  
Chain Reaction ◽  
Dot Blot Hybridization ◽  
Production Area ◽  
Olpidium Brassicae ◽  
Polymerase Chain ◽  
Production Areas

Nonisotopic molecular dot blot hybridization technique and multiplex reverse transcription-polymerase chain reaction assay for the specific detection of Lettuce big-vein virus (LBVV) and Mirafiori lettuce virus (MiLV) in lettuce tissue were developed. Both procedures were suitable for the specific detection of both viruses in a range of naturally infected lettuce plants from various Spanish production areas and seven different cultivars. The study of the distribution of both viruses in the plant revealed that the highest concentration of LBVV and MiLV occurred in roots and old leaves, respectively. LBVV infection progress in a lettuce production area was faster than that observed for MiLV. In spite of different rates of virus infection progress, most lettuce plants became infected with both viruses about 100 days posttransplant. The appearance of both viruses in lettuce crops was preceded by a peak in the concentration of resting spores and zoosporangia of the fungus vector Olpidium brassicae in lettuce roots.

Diagnosis of goose circovirus infection in Hungarian geese samples using polymerase chain reaction and dot blot hybridization tests

2004 ◽  
Vol 33 (1) ◽  
pp. 51-58 ◽  
Author(s):  
N. W. Ball ◽  
J. A. Smyth ◽  
J. H. Weston ◽  
B. J. Borghmans ◽  
V. Palya ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blot Hybridization ◽  
Dot Blot ◽  
Chain Reaction ◽  
Dot Blot Hybridization ◽  
Polymerase Chain
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