Contribution of gold nanoparticles to the catalytic DNA strand displacement in leakage reduction and signal amplification

2017 ◽  
Vol 53 (79) ◽  
pp. 10950-10953 ◽  
Author(s):  
Bei Wang ◽  
Xiang Zhou ◽  
Dongbao Yao ◽  
Xianbao Sun ◽  
Miao He ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Signal Amplification ◽  
Strand Displacement ◽  
New Model ◽  
Improve Efficiency ◽  
Leakage Reduction ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand

A new model using a gold nanoparticle (AuNP)–DNA system to constrain leakage and improve efficiency of catalytic toehold-mediated strand displacement reactions was outlined.

scholarly journals Nonenzymatic DNA-Based Fluorescence Biosensor Combining Carbon Dots and Graphene Oxide with Target-Induced DNA Strand Displacement for microRNA Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2608
Author(s):  
Yuanyuan Gao ◽  
Hong Yu ◽  
Jingjing Tian ◽  
Botao Xiao
Keyword(s):  
Graphene Oxide ◽  
Carbon Dots ◽  
Signal Amplification ◽  
Strand Displacement ◽  
Fluorescence Sensing ◽  
Microrna Detection ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand ◽  
Fluorescence Biosensor

Based on a fluorescence “on-off-on” strategy, we fabricated a simple and highly sensitive DNA-based fluorescence biosensor for the detection of micro (mi)RNA from carbon dots (CDs) and graphene oxide (GO) without complicated and time-consuming operations. CDs were successfully synthesized and conjugated to the end of a single-stranded fuel DNA that was adsorbed onto the surface of GO through π-π stacking, resulting in fluorescence quenching. In the presence of the target miRNA let-7a, the fuel DNA was desorbed from the GO surface, and fluorescence was restored through two successive toehold-mediated strand displacement reactions on double-stranded DNA-modified gold nanoparticles. The target miRNA let-7a was recycled, leading to signal amplification. The concentration of let-7a was proportional to the degree of fluorescence recovery. Under optimal conditions, there was a good linear relationship between the relative fluorescence intensity and let-7a concentration in the range of 0.01–1 nM, with a detection limit of 7.8 pM. With its advantages of signal amplification and high biocompatibility, this fluorescence sensing strategy can be applied to the detection of a variety of target miRNAs and can guide the design of novel biosensors with improved properties.

Biocomputing based on DNA strand displacement reactions

ChemPhysChem ◽  
2021 ◽  
Author(s):  
Hui Lv ◽  
Qian Li ◽  
Jiye Shi ◽  
Fei Wang ◽  
Chunhai Fan
Keyword(s):  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand

Connecting localized DNA strand displacement reactions

Nanoscale ◽  
2015 ◽  
Vol 7 (30) ◽  
pp. 12970-12978 ◽  
Author(s):  
Ismael Mullor Ruiz ◽  
Jean-Michel Arbona ◽  
Amitkumar Lad ◽  
Oscar Mendoza ◽  
Jean-Pierre Aimé ◽  
...  
Keyword(s):  
Dna Origami ◽  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand

Design and characterization of a DNA-based localized amplification circuit which, upon tethering on a DNA origami platform, greatly accelerates the catalytic response.

scholarly journals Programming colloidal bonding using DNA strand-displacement circuitry

2020 ◽  
Vol 117 (11) ◽  
pp. 5617-5623 ◽  
Author(s):  
Xiang Zhou ◽  
Dongbao Yao ◽  
Wenqiang Hua ◽  
Ningdong Huang ◽  
Xiaowei Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Thermal Annealing ◽  
Constant Temperature ◽  
Room Temperature ◽  
Dna Nanotechnology ◽  
Structural Transformations ◽  
Temperature Sensitive ◽  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Dna Strand

As a strategy for regulating entropy, thermal annealing is a commonly adopted approach for controlling dynamic pathways in colloid assembly. By coupling DNA strand-displacement circuits with DNA-functionalized colloid assembly, we developed an enthalpy-mediated strategy for achieving the same goal while working at a constant temperature. Using this tractable approach allows colloidal bonding to be programmed for synchronization with colloid assembly, thereby realizing the optimal programmability of DNA-functionalized colloids. We applied this strategy to conditionally activate colloid assembly and dynamically switch colloid identities by reconfiguring DNA molecular architectures, thereby achieving orderly structural transformations; leveraging the advantage of room-temperature assembly, we used this method to prepare a lattice of temperature-sensitive proteins and gold nanoparticles. This approach bridges two subfields: dynamic DNA nanotechnology and DNA-functionalized colloid programming.

The fast detection of streptavidin based on the initial reaction rate of the binding-induced DNA strand-displacement reaction

2016 ◽  
Vol 8 (37) ◽  
pp. 6701-6704 ◽  
Author(s):  
Chenxi Li ◽  
Ruoyun Lin ◽  
Tian Li ◽  
Feng Liu ◽  
Na Li
Keyword(s):  
Small Molecules ◽  
Reaction Rate ◽  
Initial Reaction Rate ◽  
Initial Reaction ◽  
Strand Displacement ◽  
Fast Detection ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand ◽  
Strand Displacement Reaction

Binding-induced DNA strand-displacement reactions diversify the applications beyond nucleic acids and small molecules.

Enhancing-effect of gold nanoparticles on DNA strand displacement amplifications and their application to an isothermal telomerase assay

2014 ◽  
Vol 5 (11) ◽  
pp. 4153-4162 ◽  
Author(s):  
Leilei Tian ◽  
Timothy M. Cronin ◽  
Yossi Weizmann
Keyword(s):  
Gold Nanoparticles ◽  
Dna Amplification ◽  
Strand Displacement ◽  
Enhancing Effect ◽  
Specificity And Sensitivity ◽  
Dna Strand Displacement ◽  
Amplification Assay ◽  
Dna Strand

AuNPs take the reliability of a typical isothermal DNA amplification assay to a new level of accuracy, specificity, and sensitivity.

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