Enhancing-effect of gold nanoparticles on DNA strand displacement amplifications and their application to an isothermal telomerase assay

2014 ◽  
Vol 5 (11) ◽  
pp. 4153-4162 ◽  
Author(s):  
Leilei Tian ◽  
Timothy M. Cronin ◽  
Yossi Weizmann
Keyword(s):  
Gold Nanoparticles ◽  
Dna Amplification ◽  
Strand Displacement ◽  
Enhancing Effect ◽  
Specificity And Sensitivity ◽  
Dna Strand Displacement ◽  
Amplification Assay ◽  
Dna Strand

AuNPs take the reliability of a typical isothermal DNA amplification assay to a new level of accuracy, specificity, and sensitivity.

scholarly journals Programming colloidal bonding using DNA strand-displacement circuitry

2020 ◽  
Vol 117 (11) ◽  
pp. 5617-5623 ◽  
Author(s):  
Xiang Zhou ◽  
Dongbao Yao ◽  
Wenqiang Hua ◽  
Ningdong Huang ◽  
Xiaowei Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Thermal Annealing ◽  
Constant Temperature ◽  
Room Temperature ◽  
Dna Nanotechnology ◽  
Structural Transformations ◽  
Temperature Sensitive ◽  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Dna Strand

As a strategy for regulating entropy, thermal annealing is a commonly adopted approach for controlling dynamic pathways in colloid assembly. By coupling DNA strand-displacement circuits with DNA-functionalized colloid assembly, we developed an enthalpy-mediated strategy for achieving the same goal while working at a constant temperature. Using this tractable approach allows colloidal bonding to be programmed for synchronization with colloid assembly, thereby realizing the optimal programmability of DNA-functionalized colloids. We applied this strategy to conditionally activate colloid assembly and dynamically switch colloid identities by reconfiguring DNA molecular architectures, thereby achieving orderly structural transformations; leveraging the advantage of room-temperature assembly, we used this method to prepare a lattice of temperature-sensitive proteins and gold nanoparticles. This approach bridges two subfields: dynamic DNA nanotechnology and DNA-functionalized colloid programming.

scholarly journals Highly specific SNP detection using 2D graphene electronics and DNA strand displacement

2016 ◽  
Vol 113 (26) ◽  
pp. 7088-7093 ◽  
Author(s):  
Michael T. Hwang ◽  
Preston B. Landon ◽  
Joon Lee ◽  
Duyoung Choi ◽  
Alexander H. Mo ◽  
...  
Keyword(s):  
Personalized Medicine ◽  
High Specificity ◽  
Practical Implementation ◽  
Strand Displacement ◽  
Snp Detection ◽  
Single Nucleotide ◽  
Specificity And Sensitivity ◽  
Dna Strand Displacement ◽  
Wide Range ◽  
Dna Strand

Single-nucleotide polymorphisms (SNPs) in a gene sequence are markers for a variety of human diseases. Detection of SNPs with high specificity and sensitivity is essential for effective practical implementation of personalized medicine. Current DNA sequencing, including SNP detection, primarily uses enzyme-based methods or fluorophore-labeled assays that are time-consuming, need laboratory-scale settings, and are expensive. Previously reported electrical charge-based SNP detectors have insufficient specificity and accuracy, limiting their effectiveness. Here, we demonstrate the use of a DNA strand displacement-based probe on a graphene field effect transistor (FET) for high-specificity, single-nucleotide mismatch detection. The single mismatch was detected by measuring strand displacement-induced resistance (and hence current) change and Dirac point shift in a graphene FET. SNP detection in large double-helix DNA strands (e.g., 47 nt) minimize false-positive results. Our electrical sensor-based SNP detection technology, without labeling and without apparent cross-hybridization artifacts, would allow fast, sensitive, and portable SNP detection with single-nucleotide resolution. The technology will have a wide range of applications in digital and implantable biosensors and high-throughput DNA genotyping, with transformative implications for personalized medicine.

Contribution of gold nanoparticles to the catalytic DNA strand displacement in leakage reduction and signal amplification

2017 ◽  
Vol 53 (79) ◽  
pp. 10950-10953 ◽  
Author(s):  
Bei Wang ◽  
Xiang Zhou ◽  
Dongbao Yao ◽  
Xianbao Sun ◽  
Miao He ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Signal Amplification ◽  
Strand Displacement ◽  
New Model ◽  
Improve Efficiency ◽  
Leakage Reduction ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand

A new model using a gold nanoparticle (AuNP)–DNA system to constrain leakage and improve efficiency of catalytic toehold-mediated strand displacement reactions was outlined.

scholarly journals A Universal Fast Colorimetric Method for DNA Signal Detection with DNA Strand Displacement and Gold Nanoparticles

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Xin Li ◽  
Tao Song ◽  
Zhihua Chen ◽  
Xiaolong Shi ◽  
Congzhou Chen ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Signal Detection ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Strand Displacement ◽  
Target Dna ◽  
Dna Strand Displacement ◽  
Or Gene ◽  
Dna Strand ◽  
Visual Results

DNA or gene signal detection is of great significance in many fields including medical examination, intracellular molecular monitoring, and gene disease signal diagnosis, but detection of DNA or gene signals in a low concentration with instant visual results remains a challenge. In this work, a universal fast and visual colorimetric detection method for DNA signals is proposed. Specifically, a DNA signal amplification “circuit” based on DNA strand displacement is firstly designed to amplify the target DNA signals, and then thiol modified hairpin DNA strands and gold nanoparticles are used to make signal detection results visualized in a colorimetric manner. If the target DNA signal exists, the gold nanoparticles aggregate and settle down with color changing from dark red to grey quickly; otherwise, the gold nanoparticles’ colloids remain stable in dark red. The proposed method provides a novel way to detect quickly DNA or gene signals in low concentrations with instant visual results. When applied in real-life, it may provide a universal colorimetric method for gene disease signal diagnosis.

Control of gold nanoparticles based on circular DNA strand displacement

2014 ◽  
Vol 418 ◽  
pp. 31-36 ◽  
Author(s):  
Cheng Zhang ◽  
Jingjing Ma ◽  
Jing Yang ◽  
Yafei Dong ◽  
Jin Xu
Keyword(s):  
Gold Nanoparticles ◽  
Strand Displacement ◽  
Circular Dna ◽  
Dna Strand Displacement ◽  
Dna Strand

scholarly journals Kinetics of DNA Strand Displacement Systems with Locked Nucleic Acids

2017 ◽  
Vol 121 (12) ◽  
pp. 2594-2602 ◽  
Author(s):  
Xiaoping Olson ◽  
Shohei Kotani ◽  
Bernard Yurke ◽  
Elton Graugnard ◽  
William L. Hughes
Keyword(s):  
Nucleic Acids ◽  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Locked Nucleic Acids ◽  
Dna Strand ◽  
Kinetics Of

Biocomputing based on DNA strand displacement reactions

ChemPhysChem ◽  
2021 ◽  
Author(s):  
Hui Lv ◽  
Qian Li ◽  
Jiye Shi ◽  
Fei Wang ◽  
Chunhai Fan
Keyword(s):  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Displacement Reactions ◽  
Dna Strand

scholarly journals Kinetics of Toehold-Mediated DNA Strand Displacement Depend on FeII4L4 Tetrahedron Concentration

Nano Letters ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 1368-1374
Author(s):  
Jinbo Zhu ◽  
Filip Bošković ◽  
Bao-Nguyen T. Nguyen ◽  
Jonathan R. Nitschke ◽  
Ulrich F. Keyser
Keyword(s):  
Strand Displacement ◽  
Dna Strand Displacement ◽  
Dna Strand ◽  
Kinetics Of

Highly sensitive multiplex detection of microRNA by competitive DNA strand displacement fluorescence assay

Talanta ◽  
2019 ◽  
Vol 200 ◽  
pp. 487-493 ◽  
Author(s):  
Raja Chinnappan ◽  
Rawa Mohammed ◽  
Ahmed Yaqinuddin ◽  
Khalid Abu-Salah ◽  
Mohammed Zourob
Keyword(s):  
Fluorescence Assay ◽  
Multiplex Detection ◽  
Strand Displacement ◽  
Highly Sensitive ◽  
Dna Strand Displacement ◽  
Dna Strand
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