Fluorescence-switching RNA for detection of bacterial ribosomes

2017 ◽  
Vol 53 (68) ◽  
pp. 9406-9409
Author(s):  
Lihao Guo ◽  
Akimitsu Okamoto

The fluorescence emission specific to bacterial ribosomes from a turn-on-type fluorescent RNA enabled quantification of bacterial population.

2013 ◽  
Vol 864-867 ◽  
pp. 672-676
Author(s):  
Li Zhang ◽  
Da Quan Zhang ◽  
Yu Meng ◽  
Li Xin Gao

A rhodamine-based fluorescent compound, spiro[1H-isoindole-1,9′-[9H]xanthen] -3(2H)-one, ′,6′-bis(diethylamino)-2-[(1-methylethylidene)-amino] (RB1), is developed to detect and inhibit the refractory steel corrosion in 3% NaCl solution. The inhibition effect was evaluated by electrochemical measurements. RB1 suppresses the cathodic corrosion reaction and shifts the corrosion potential toward more negative values. RB1 exhibits a turn-on fluorescence emission in the presence of Fe 3+ ions. The selectivity of RB1 over other commonly coexistent Cu2+, Fe 2+ and Mn2+ was investigated. The variation of the fluorescence intensity is correlated to that of weight-loss. RB1 has a potential application as corrosion inhibitor and corrosion indicator in some industry process.


Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2889 ◽  
Author(s):  
Ye Teng ◽  
Hisae Tateishi-Karimata ◽  
Takaaki Tsuruoka ◽  
Naoki Sugimoto

Nucleic acid stability and structure, which are crucial to the properties of fluorescent DNA-templated silver nanoclusters (DNA-Ag NCs), significantly change in ionic liquids. In this work, our purpose was to study DNA-Ag NCs in a buffer containing the hydrated ionic liquid of choline dihydrogen phosphate (choline dhp) to improve fluorescence for application in DNA detection. Due to the stabilisation of an i-motif structure by the choline cation, a unique fluorescence emission—that was not seen in an aqueous buffer—was observed in choline dhp and remained stable for more than 30 days. A DNA-Ag NCs probe was designed to have greater fluorescence intensity in choline dhp in the presence of a target DNA. A turn-on sensing platform in choline dhp was built for the detection of the BRCA1 gene, which is related to familial breast and ovarian cancers. This platform showed better sensitivity and selectivity in distinguishing a target sequence from a mutant sequence in choline dhp than in the aqueous buffer. Our study provides new evidence regarding the effects of structure on properties of fluorescent DNA-Ag NCs and expands the applications of fluorescent DNA-Ag NCs in an ionic liquid because of improved sensitivity and selectivity.


2020 ◽  
Vol 19 (12) ◽  
pp. 1690-1696
Author(s):  
Jian-Peng Hu ◽  
Hao-Hang Yang ◽  
Qi Lin ◽  
Hong Yao ◽  
You-Ming Zhang ◽  
...  

Herein, we synthesized a trimesoyl chloride–rhodamine derivative TR. TR exhibited metal ions complexation induced photophysical ‘turn-on’ signaling responses to Hg2+ and Fe3+.


2019 ◽  
Vol 7 (33) ◽  
pp. 19471-19484 ◽  
Author(s):  
Ranadip Goswami ◽  
Shyama Charan Mandal ◽  
Nilanjan Seal ◽  
Biswarup Pathak ◽  
Subhadip Neogi

Mixed-linker CSMCRI-2 demonstrates the turn-on and nanomolar detection of antibiotics and biophosphates with antibiotic-triggered reversible fluorescence switching and keypad lock function.


NANO ◽  
2017 ◽  
Vol 12 (02) ◽  
pp. 1750024 ◽  
Author(s):  
Xiqing Liu ◽  
Xiao Wei ◽  
Yeqing Xu ◽  
Hongji Li ◽  
Kai Lu ◽  
...  

In this paper, a novel fluorescent nanoswitch based on carbon dots (CDs) was developed for the sensitive and selective determination of Hg[Formula: see text] and I[Formula: see text]. The CDs were obtained by simple hydrothermal process and had a strong fluorescence emission at 440[Formula: see text]nm. The fluorescence of the CDs can be selectively quenched by Hg[Formula: see text] ion, and then the I[Formula: see text] was added into the system, which can interact with Hg[Formula: see text] and recover fluorescence of the CDs. Under optimal conditions, the quenching fluorescence intensity on addition of Hg[Formula: see text] has obtained a satisfactory linear relationship covering the linear range of 0–50[Formula: see text][Formula: see text]M with the linear relationship ([Formula: see text]), and the limit of detection is 0.047[Formula: see text][Formula: see text]M. The additions of I[Formula: see text] could lead to the fluorescence intensity of the solution of CDs and Hg[Formula: see text] (50[Formula: see text][Formula: see text]M) recover rapidly, which is linearly related ([Formula: see text]) to the concentration of I[Formula: see text] in the range from 0 to 70[Formula: see text][Formula: see text]M, the detection limit for I[Formula: see text] was calculated to be 0.084[Formula: see text][Formula: see text]M. Moreover, the developed method to detect Hg[Formula: see text] and I[Formula: see text] was evaluated in real examples, and the fluorescence switching can sensitively and selectively detect Hg[Formula: see text] and I[Formula: see text] over some potentially interfering ions, the recoveries were up to 97.8–107.0% and 96.7–106.6%, respectively.


2019 ◽  
Vol 11 (32) ◽  
pp. 4190-4196 ◽  
Author(s):  
Jagpreet Singh Sidhu ◽  
Shilpa Sharma ◽  
Ashutosh Singh ◽  
Neha Garg ◽  
Navneet Kaur ◽  
...  

Fluorescence emission of the receptor enhanced upon its binding with uric acid due to the cancellation of the PET phenomenon.


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