scholarly journals Cell density overrides the effect of substrate stiffness on human mesenchymal stem cells’ morphology and proliferation

2018 ◽  
Vol 6 (5) ◽  
pp. 1109-1119 ◽  
Author(s):  
Balu Venugopal ◽  
Pankaj Mogha ◽  
Jyotsna Dhawan ◽  
Abhijit Majumder
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Density ◽  
Cellular Response ◽  
Human Mesenchymal Stem Cells ◽  
Cell Interaction ◽  
Substrate Stiffness ◽  
Substrate Rigidity ◽  
Cell Cell

Cell–cell interactionviasubstrate deformation in turn modifies cellular response to substrate rigidity.

Adhesion of hematopoietic progenitor cells to human mesenchymal stem cells as a model for cell−cell interaction

2007 ◽  
Vol 35 (2) ◽  
pp. 314-325 ◽  
Author(s):  
Wolfgang Wagner ◽  
Frederik Wein ◽  
Christoph Roderburg ◽  
Rainer Saffrich ◽  
Anne Faber ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Progenitor Cells ◽  
Human Mesenchymal Stem Cells ◽  
Cell Interaction ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor ◽  
Cell Cell

scholarly journals A newly identified mechanism involved in regulation of human mesenchymal stem cells by fibrous substrate stiffness

2016 ◽  
Vol 42 ◽  
pp. 247-257 ◽  
Author(s):  
Huihua Yuan ◽  
Yaxian Zhou ◽  
Ming-Song Lee ◽  
Yanzhong Zhang ◽  
Wan-Ju Li
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Substrate Stiffness

scholarly journals Effects of substrate stiffness on the tenoinduction of human mesenchymal stem cells

2017 ◽  
Vol 58 ◽  
pp. 244-253 ◽  
Author(s):  
Anowarul Islam ◽  
Thomas Mbimba ◽  
Mousa Younesi ◽  
Ozan Akkus
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Substrate Stiffness

scholarly journals Effects of matrix elasticity and cell density on human mesenchymal stem cells differentiation

2013 ◽  
Vol 31 (9) ◽  
pp. 1360-1365 ◽  
Author(s):  
Ruyue Xue ◽  
Julie Yi-Shuan Li ◽  
Yiting Yeh ◽  
Li Yang ◽  
Shu Chien
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Density ◽  
Human Mesenchymal Stem Cells ◽  
Matrix Elasticity

Substrate stiffness and sequence dependent bioactive peptide hydrogels influence the chondrogenic differentiation of human mesenchymal stem cells

2021 ◽  
Author(s):  
Mohammed Mohiuddin ◽  
Zong-Sheng Lai ◽  
Hsin-Chieh Lin
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Crucial Role ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Bioactive Peptide ◽  
Substrate Stiffness ◽  
Transmembrane Glycoprotein ◽  
Biochemical Signals ◽  
Peptide Hydrogels

N-Cadherin is a transmembrane glycoprotein that plays a crucial role in the condensation of mesenchymal cells by enhancing cell-cell interactions during the process of chondrogenesis. The biophysical and biochemical signals...

scholarly journals Substrate stiffness controls the cell cycle of human mesenchymal stem cells via cellular traction

2021 ◽  
Author(s):  
Sanjay Kumar Kureel ◽  
Shatarupa Sinha ◽  
Purboja Purkayastha ◽  
Sarah Barretto ◽  
Abhijit Majumder
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Cell Types ◽  
Contractile Proteins ◽  
Substrate Stiffness ◽  
Rock Inhibitor ◽  
Spread Area ◽  
Contractile Forces

The microenvironment of human mesenchymal stem cells (hMSCs) regulates their self-renewal and differentiation properties. Previously it was shown that hMSCs remained quiescent on soft (0.25 kPa) polyacrylamide (PA) gels but re-entered into cell cycle on a stiff (7.5 kPa) gel. However, how cells behave on intermediate stiffness and what intracellular factors transmit mechanical changes to cell interior thereby regulating cell cycle remained unknown. In this work we demonstrated that PA gels between 1 and 5 kPa act as a mechanical switch in regulating cell cycle of hMSCs. By experiments on cell-cycle exit and re-entry, we found that hMSCs demonstrated a sharp transition from quiescence to proliferation between 1 and 5 kPa. Further studies with ROCK inhibitor Y-27632 revealed that contractile proteins, but not cell spread area, accounts for the sensitivity of hMSCs towards substrate stiffness and hence correlates with their changes in cell cycle. These observations therefore suggest that substrate stiffness regulates hMSC proliferation through contractile forces as generated by cellular contractile proteins in a unique pattern which is distinct from other cell types as studied.

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