scholarly journals Human Mesenchymal Stem Cells Inhibit Endothelial Proliferation and Angiogenesis via Cell–Cell Contact Through Modulation of the VE-Cadherin/β-Catenin Signaling Pathway

2013 ◽  
Vol 22 (1) ◽  
pp. 148-157 ◽  
Author(s):  
Tyler Menge ◽  
Michael Gerber ◽  
Kathryn Wataha ◽  
William Reid ◽  
Sushovan Guha ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Signaling Pathway ◽  
Human Mesenchymal Stem Cells ◽  
Cell Contact ◽  
Endothelial Proliferation ◽  
Cell Cell

scholarly journals Bone marrow mesenchymal stem cells promote prostate cancer cell stemness via cell–cell contact to activate the Jagged1/Notch1 pathway

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ji-wen Cheng ◽  
Li-xia Duan ◽  
Yang Yu ◽  
Pu Wang ◽  
Jia-le Feng ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Western Blot ◽  
Cell Contact ◽  
Activity Levels ◽  
Tumor Resistance ◽  
Cell Cell

Abstract Background Mesenchymal stem cells (MSCs) play a crucial role in cancer development and tumor resistance to therapy in prostate cancer, but the influence of MSCs on the stemness potential of PCa cells by cell–cell contact remains unclear. In this study, we investigated the effect of direct contact of PCa cells with MSCs on the stemness of PCa and its mechanisms. Methods First, the flow cytometry, colony formation, and sphere formation were performed to determine the stemness of PCaMSCs, and the expression of stemness-related molecules (Sox2, Oct4, and Nanog) was investigated by western blot analysis. Then, we used western blot and qPCR to determine the activity levels of two candidate pathways and their downstream stemness-associated pathway. Finally, we verified the role of the significantly changed pathway by assessing the key factors in this pathway via in vitro and in vivo experiments. Results We established that MSCs promoted the stemness of PCa cells by cell–cell contact. We here established that the enhanced stemness of PCaMSCs was independent of the CCL5/CCR5 pathway. We also found that PCaMSCs up-regulated the expression of Notch signaling-related genes, and inhibition of Jagged1-Notch1 signaling in PCaMSCs cells significantly inhibited MSCs-induced stemness and tumorigenesis in vitro and in vivo. Conclusions Our results reveal a novel interaction between MSCs and PCa cells in promoting tumorigenesis through activation of the Jagged1/Notch1 pathway, providing a new therapeutic target for the treatment of PCa.

TNFα promotes osteogenic differentiation of human mesenchymal stem cells by triggering the NF-κB signaling pathway

Bone ◽  
2009 ◽  
Vol 45 (2) ◽  
pp. 367-376 ◽  
Author(s):  
Katrin Hess ◽  
Alexey Ushmorov ◽  
Jörg Fiedler ◽  
Rolf E. Brenner ◽  
Thomas Wirth
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Signaling Pathway ◽  
Human Mesenchymal Stem Cells

Flavonoids of Herba Epimedii regulate osteogenesis of human mesenchymal stem cells through BMP and Wnt/β-catenin signaling pathway

2010 ◽  
Vol 314 (1) ◽  
pp. 70-74 ◽  
Author(s):  
Jin-fang Zhang ◽  
Guo Li ◽  
Chu-yan Chan ◽  
Chun-ling Meng ◽  
Marie Chia-mi Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Signaling Pathway ◽  
Human Mesenchymal Stem Cells ◽  
Herba Epimedii

Molecular Composition of Intercellular Contacts in Human Mesenchymal Stem Cells.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2332-2332
Author(s):  
Patrick Wuchter ◽  
Judit Boda ◽  
Beate Straub ◽  
Ulf Krause ◽  
Anja Seckinger ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Gap Junctions ◽  
Light And Electron Microscopy ◽  
Human Mesenchymal Stem Cells ◽  
Molecular Composition ◽  
Cell Contact ◽  
Intercellular Contacts ◽  
Adhering Junctions

Abstract The long-term fate of stem cells depends on their interaction with the niche. Mesenchymal stem cells (MSC) from human bone marrow have been demonstrated to differentiate into various tissues, such as bone, cartilage, muscle and fat. As the interaction with the microenvironment plays a major role in differentiation, we have characterized the cell-cell contact among MSC. We have demonstrated the occurrence of a novel kind of adhering junction, consisting of slender, villiform-to-vermiform cell projections (processus adhaerentes). In this study, we have systematically analyzed the molecular composition of these junctions. A panel of antibodies specific for various components of tight junctions, gap junctions, adherens junctions and desmosomes was used. The expression of these antigens was verified by light and electron microscopy and by biochemical analysis, including immunoprecipitation and RT-PCR. MSC from two different sources were analyzed: MSC obtained from bone marrow aspirates from healthy voluntary donors and additionally MSC originating from umbilical cord blood donated for scientific research. We have also shown the presence of vimentin-positive retothelial cells (which are probably the source of MSC) in situ in fresh bone marrow samples. We demonstrate that MSC were interconnected by occasional gap junctions and frequent adhering junctions. Additionally, we found a unique molecular composition of these adhering junctions, as they comprise the transmembrane glycoproteins cadherin 11, N-cadherin and syndecan-1, together with the cytoplasmic plaque proteins α- and β-catenin and p120ctn. Constitutive complexes of these molecules have been identified. Our data indicate that MSC communicate with each other through junctions and junctional complexes. We hypothesize that MSC can embark on alternative differentiation pathways with specific junctional and cytoskeletal patterns. Characterization of and understanding the role of such intercellular contacts and their correlation to specific differentiation programs are being conducted.

Diabetic sera disrupted the normal exosome signaling pathway in human mesenchymal stem cells in vitro

2018 ◽  
Vol 374 (3) ◽  
pp. 555-565 ◽  
Author(s):  
Jafar Rezaie ◽  
Vahid Nejati ◽  
Majid Khaksar ◽  
Ahmad Oryan ◽  
Nasser Aghamohamadzadeh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Signaling Pathway ◽  
Human Mesenchymal Stem Cells

scholarly journals Contribution of the Interleukin-6/STAT-3 Signaling Pathway to Chondrogenic Differentiation of Human Mesenchymal Stem Cells

2015 ◽  
Vol 67 (5) ◽  
pp. 1250-1260 ◽  
Author(s):  
Masahiro Kondo ◽  
Kunihiro Yamaoka ◽  
Kei Sakata ◽  
Koshiro Sonomoto ◽  
Lin Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Signaling Pathway ◽  
Interleukin 6 ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cells
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