Simultaneous detection of superoxide anion radicals and determination of the superoxide scavenging activity of antioxidants using a N,N-dimethyl-p-phenylene diamine/Nafion colorimetric sensor

2017 ◽  
Vol 9 (43) ◽  
pp. 6202-6212 ◽  
Author(s):  
Ferda Dondurmacıoğlu ◽  
Aslı Neslihan Avan ◽  
Reşat Apak
Keyword(s):  
Superoxide Anion ◽  
Simultaneous Detection ◽  
Colorimetric Sensor ◽  
Superoxide Radicals ◽  
Scavenging Activity ◽  
Superoxide Scavenging Activity ◽  
Superoxide Anion Radicals ◽  
Phenylene Diamine ◽  
Anion Radicals

A novel colorimetric DMPD assay was developed for superoxide radicals generated by the NADH/PMS/O2 system, and compared with conventional NBT assay.

scholarly journals Preparation of Keratin-Glycine Metal Complexes and Their Scavenging Activity for Superoxide Anion Radicals

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Jianfeng Wang ◽  
Xiaoxiao Li ◽  
Yufeng He ◽  
Pengfei Song ◽  
Rong-Min Wang
Keyword(s):  
Metal Ions ◽  
Metal Complexes ◽  
Superoxide Anion ◽  
Scavenging Activity ◽  
Feather Keratin ◽  
Limited Application ◽  
New Type ◽  
Ft Ir ◽  
Superoxide Anion Radicals ◽  
Anion Radicals

To address the problem of limited application of natural SOD, the development of SOD mimic enzymes is of great importance for bioantioxidation. Herein, we report on a new type of biopolymer antioxidant with excellent scavenging activity for O2•-, keratin-glycine metal complexes (FK-GlyM, M = Zn, Cu, Mn, Ni). They are prepared by feather keratin firstly combined with glycine and then metal ions. Using FT-IR, TG, CD, and SEM, the performance of the obtained complexes (FK-GlyM) for scavenging O2•- is analysed and investigated. Importantly, the scavenging activity of FK-GlyCu is excellent in all FK-GlyM, and FK-GlyCu60 has the most excellent anti-O2•- activity in all FK-GlyCux, of which EC50 and degree of simulation were, respectively, up to 4.5×10-3±0.0012 μmol/L and 911.1% compared with nature Cu, Zn-SOD. Finally, its mechanism was also discussed. In summary, this method about the simulation strategies will provide a novel idea for exploiting new-type biocompatible and highly reactive antioxidants.

scholarly journals Inclusion Complexes of Lycopene and β-Cyclodextrin: Preparation, Characterization, Stability and Antioxidant Activity

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 314 ◽  
Author(s):  
Haixiang Wang ◽  
Shaofeng Wang ◽  
Hua Zhu ◽  
Suilou Wang ◽  
Jiudong Xing
Keyword(s):  
Antioxidant Activity ◽  
Inclusion Complexes ◽  
Superoxide Anion ◽  
Antioxidant Properties ◽  
Phase Solubility ◽  
Scavenging Activity ◽  
Positive Effects ◽  
Phase Solubility Study ◽  
Superoxide Anion Radicals ◽  
Anion Radicals

In this study, the inclusion complexes of lycopene with β-cyclodextrin (β-CD) were prepared by the precipitation method. Then the inclusion complexes were characterized by the scanning electron microscopy (SEM), ultraviolet-visible spectroscopy (UV), microscopic observation, liquid chromatography, differential scanning calorimetry (DSC) and phase-solubility study. Moreover, the stability and antioxidant activity were tested. The results showed that lycopene was embedded into the cavity of β-CD with a 1:1 stoichiometry. Moreover, the thermal and irradiant stabilities of lycopene were all significantly increased by the formation of lycopene/β-CD inclusion complexes. Antioxidant properties of lycopene and its inclusion complexes were evaluated on the basis of measuring the scavenging activity for 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl and superoxide anion radicals. The results showed that the scavenging activity of DPPH radicals was obviously increased by the formation of the inclusion complex with β-cyclodextrin at concentrations of 5–30 μg/mL, however, some significant positive effects on the scavenging activity of hydroxyl and superoxide anion radicals were not observed and the reasons are worth further study.

scholarly journals Determination of superoxide anion radicals generated by human polymorphonuclear leukocytes using spin trapping technique.

Ensho ◽  
1989 ◽  
Vol 9 (4) ◽  
pp. 323-327
Author(s):  
Yuji Naito ◽  
Toshikazu Yoshikawa ◽  
Toru Tanigawa ◽  
Hirokazu Oyamada ◽  
Shigenobu Ueda ◽  
...  
Keyword(s):  
Superoxide Anion ◽  
Polymorphonuclear Leukocytes ◽  
Spin Trapping ◽  
Human Polymorphonuclear Leukocytes ◽  
Spin Trapping Technique ◽  
Superoxide Anion Radicals ◽  
Anion Radicals
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