Direct quantitative analysis of the natural moisturizing factor (NMF) in the stratum corneum by direct analysis in real time mass spectrometry (DART-MS)

2017 ◽  
Vol 9 (33) ◽  
pp. 4851-4857 ◽  
Author(s):  
Katsuyuki Maeno ◽  
Yasuo Shida ◽  
Haruo Shimada
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Quantitative Analysis ◽  
Carboxylic Acid ◽  
Stratum Corneum ◽  
Real Time ◽  
Direct Analysis ◽  
Urocanic Acid ◽  
Pyrrolidone Carboxylic Acid

Analyzing the natural moisturizing factor (NMF) in the stratum corneum (SC), such as amino acids, pyrrolidone carboxylic acid, and urocanic acid, is important in dermatology research.

Ionization characteristics of amino acids in direct analysis in real time mass spectrometry

The Analyst ◽  
2014 ◽  
Vol 139 (10) ◽  
pp. 2589-2599 ◽  
Author(s):  
Kanako Sekimoto ◽  
Motoshi Sakakura ◽  
Takatomo Kawamukai ◽  
Hiroshi Hike ◽  
Teruhisa Shiota ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Real Time ◽  
Direct Analysis ◽  
Dart Mass Spectrometry ◽  
Hydrogen Radicals

Analytes used in DART mass spectrometry can be oxidized by hydrogen radicals HO˙ via oxygen attachment and hydrogen loss.

scholarly journals Direct Quantification of Natural Moisturizing Factors in Stratum Corneum using Direct Analysis in Real Time Mass Spectrometry with Inkjet-Printing Technique

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Katsuyuki Maeno
Keyword(s):  
Mass Spectrometry ◽  
Stratum Corneum ◽  
Real Time ◽  
Inkjet Printing ◽  
Internal Standard ◽  
Direct Analysis ◽  
Water Soluble ◽  
Analytical Technique ◽  
Printing Technique ◽  
Linear Correlations

AbstractProper hydration of the stratum corneum, the skin’s outermost layer, is essential for healthy skin. Water-soluble substances called natural moisturizing factors (NMF) are responsible for maintaining adequate moisture in the skin and are closely associated with a variety of the skin’s functions. Therefore, quantitative analysis methods for NMF are indispensable when attempting to clarify one of the mechanisms of hydration and its effect on the skin. This study sought to develop a quick and simple analytical technique, which can quantify NMF from the skin without the need for extraction or separation, using direct analysis in real time-mass spectrometry (DART-MS). The goal was to deliver a high quantitative capability, so a unique inkjet printing technique was employed to evenly coat a stable isotope-labeled internal standard (SIL-IS) on tape-stripped skin. This technique allowed for the quantification of 26 NMF with established calibration curves and comparatively high linear correlations. The speed of measurement was found to be advantageous as 100 strips of tape can be measured in roughly 2 hours. The effectiveness of the inkjet coating was also verified by comparing its precision with that of conventional pipetting. This new technique can be an alternative method to quantify NMF rapidly and perhaps allow for a clearer elucidation of their function in skin.

Determination of T-2 and HT-2 toxins from maize by direct analysis in real time mass spectrometry

2015 ◽  
Vol 8 (4) ◽  
pp. 489-497 ◽  
Author(s):  
M. Busman ◽  
C.M. Maragos
Keyword(s):  
Mass Spectrometry ◽  
Quantitative Analysis ◽  
Real Time ◽  
Accurate Determination ◽  
Internal Standard ◽  
Direct Analysis ◽  
Ionisation Mass Spectrometry ◽  
Relative Standard ◽  
Maize Sample

Direct analysis in real time (DART) ionisation coupled to mass spectrometry (MS) was used for the rapid quantitative analysis of T-2 toxin (T-2) and the related HT-2 toxin (HT-2), extracted from maize. Sample preparation procedures and instrument parameters were optimised to obtain sensitive and accurate determination of the toxins. The lowest calibration levels were 50 μg/kg for T-2 and 300 μg/kg for HT-2. Quantitative analysis was performed with the use of matrix-matched standards employing the 13C-labelled internal standard for T-2. DART-MS of maize extracts spiked with T-2 gave a linear response over the range of 50-1000 μg/kg. With the isotope dilution technique, good recoveries (99-110%) and repeatabilities (relative standard deviaiton 7.4-11.6%) were obtained at T-2 spiking levels of 100 and 1000 μg/kg. Adaptability of the developed method was demonstrated by analysis of T-2 and HT-2 from an oat flour quality control material. The results here further indicate the potential for application of ambient ionisation mass spectrometry to provide accurate, convenient quantitation of mycotoxins from grains.

Determination of ochratoxin A in wine by direct analysis in real time-tandem mass spectrometry

2017 ◽  
Vol 35 (2) ◽  
pp. 185 ◽  
Author(s):  
Xiaoming GONG ◽  
Ronghui MA ◽  
Hongtao WANG ◽  
Liqiang GUO ◽  
Kai LI ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Real Time ◽  
Ochratoxin A ◽  
Direct Analysis ◽  
Tandem Mass

scholarly journals Thermal Properties, Isothermal Decomposition by Direct Analysis in Real-Time-Of-Flight Mass Spectrometry and Non Isothermal Crystallization Kinetics of Poly(Ethylene-Co-Vinyl Alcohol)/Poly(ε-Caprolactone) Blend

Crystals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 292
Author(s):  
Abdulaziz Ali Alghamdi ◽  
Hussain Alattas ◽  
Waseem Sharaf Saeed ◽  
Abdel-Basit Al-Odayni ◽  
Ahmed Yacine Badjah Hadj Ahmed ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Crystallization Kinetics ◽  
Isothermal Crystallization ◽  
Vinyl Alcohol ◽  
Time Of Flight ◽  
Direct Analysis ◽  
Isothermal Crystallization Kinetics ◽  
Flight Mass Spectrometry ◽  
Poly Ethylene

A series of poly(ethylene-co-vinyl alcohol)/poly(ε-caprolactone) blends with different compositions were prepared using solvent casting. The miscibility of this pair of polymers was investigated using differential scanning calorimetry (DSC), and proved by a negative Flory interaction parameter value calculated from the Nishi–Wang equation. The miscibility of this blend was also confirmed by scanning electronic microscopy (SEM). The thermal behaviors of the obtained materials were investigated by DSC, thermogravimetric analysis, and direct analysis in real-time–time-of-flight mass spectrometry and the results obtained were very relevant. Furthermore, the crystalline properties of the obtained materials were studied by DSC and X-ray diffraction where the Ozawa approach was adopted to investigate the non-isothermal crystallization kinetics. The results obtained revealed that this approach described the crystallization process well.

Sign in / Sign up

Export Citation Format

Share Document