Functionalization of gold nanoparticles with nanobodies through physical adsorption

2017 ◽  
Vol 9 (23) ◽  
pp. 3430-3440 ◽  
Author(s):  
Julie Goossens ◽  
Hla Sein ◽  
Shaohong Lu ◽  
Magdalena Radwanska ◽  
Serge Muyldermans ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Ionic Strength ◽  
Protein Engineering ◽  
Physical Adsorption ◽  
Adsorption Behaviour ◽  
First Time

The physical adsorption behaviour of nanobodies to gold nanoparticles is described for the first time in an extensive manner including parameters such as pI, ionic strength and several protein engineering strategies. The generated nanobody-gold conjugates are functional.

Synthesis of a silymarin-gold nanoparticle conjugate and analysis of its liver-protecting activity

2021 ◽  
Vol 22 ◽  
Author(s):  
Sergey Staroverov ◽  
Sergey Kozlov ◽  
Alexander Fomin ◽  
Konstantib Gabalov ◽  
Alexey Volkov ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Liver Disease ◽  
Carbon Tetrachloride ◽  
Glutathione Depletion ◽  
Laboratory Animals ◽  
Experimental Hepatitis ◽  
Acute Liver Disease ◽  
The Mean ◽  
Extent Of Damage ◽  
First Time

Background: The liver disease problem prompts investigators to search for new methods of liver treatment. Introduction: Silymarin (Sil) protects the liver by reducing the concentration of free radicals and the extent of damage to the cell membranes. A particularly interesting method to increase the bioavailability of Sil is to use synthesized gold nanoparticles (AuNPs) as reagents. The study considered whether it was possible to use the silymarin-AuNP conjugate as a potential liver-protecting drug. Method: AuNPs were conjugated to Sil and examine the liver-protecting activity of the conjugate. Experimental hepatitis and hepatocyte cytolysis after carbon tetrachloride actionwere used as a model system, and the experiments were conducted on laboratory animals. Result: For the first time, silymarin was conjugated to colloidal gold nanoparticles (AuNPs). Electron microscopy showed that the resultant preparations were monodisperse and that the mean conjugate diameter was 18–30 nm ± 0.5 nm (mean diameter of the native nanoparticles, 15 ± 0.5 nm). In experimental hepatitis in mice, conjugate administration interfered with glutathione depletion in hepatocytes in response to carbon tetrachloride was conducive to an increase in energy metabolism, and stimulated the monocyte–macrophage function of the liver. The results were confirmed by the high respiratory activity of the hepatocytes in cell culture. Conclusion: We conclude that the silymarin-AuNP conjugate holds promise as a liver-protecting agent in acute liver disease caused by carbon tetrachloride poisoning.

scholarly journals Phosphatidylserine-Gold Nanoparticles (PS-AuNP) Induce Prostate and Breast Cancer Cell Apoptosis

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1094
Author(s):  
Allan Radaic ◽  
Nam E. Joo ◽  
Soo-Hwan Jeong ◽  
Seong-II Yoo ◽  
Nicholas Kotov ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gold Nanoparticles ◽  
Biomedical Applications ◽  
Therapeutic Potential ◽  
Control Treatment ◽  
Track Record ◽  
Males And Females ◽  
Breast And Prostate Cancer ◽  
First Time

Prostate and breast cancer are the current leading causes of new cancer cases in males and females, respectively. Phosphatidylserine (PS) is an essential lipid that mediates macrophage efferocytosis and is dysregulated in tumors. Therefore, developing therapies that selectively restore PS may be a potential therapeutic approach for carcinogenesis. Among the nanomedicine strategies for delivering PS, biocompatible gold nanoparticles (AuNPs) have an extensive track record in biomedical applications. In this study, we synthesized biomimetic phosphatidylserine-caped gold nanoparticles (PS-AuNPs) and tested their anticancer potential in breast and prostate cancer cells in vitro. We found that both cell lines exhibited changes in cell morphology indicative of apoptosis. After evaluating for histone-associated DNA fragments, a hallmark of apoptosis, we found significant increases in DNA fragmentation upon PS-AuNP treatment compared to the control treatment. These findings demonstrate the use of phosphatidylserine coupled with gold nanoparticles as a potential treatment for prostate and breast cancer. To the best of our knowledge, this is the first time that a phosphatidylserine-capped AuNP has been examined for its therapeutic potential in cancer therapy.

scholarly journals Facile Green, Room-Temperature Synthesis of Gold Nanoparticles Using Combretum erythrophyllum Leaf Extract: Antibacterial and Cell Viability Studies against Normal and Cancerous Cells

Antibiotics ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 893
Author(s):  
Olufunto T. Fanoro ◽  
Sundararajan Parani ◽  
Rodney Maluleke ◽  
Thabang C. Lebepe ◽  
Jose R. Varghese ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Cell Viability ◽  
Leaf Extract ◽  
Room Temperature ◽  
Klebsiella Oxytoca ◽  
Cost Effective ◽  
Average Diameter ◽  
Antibacterial Activities ◽  
Inhibition Concentration ◽  
First Time

We herein report a facile, green, cost-effective, plant-mediated synthesis of gold nanoparticles (AuNPs) for the first time using Combretum erythrophyllum (CE) plant leaves. The synthesis was conducted at room temperature using CE leaf extract serving as a reducing and capping agent. The as-synthesized AuNPs were found to be crystalline, well dispersed, and spherical in shape with an average diameter of 13.20 nm and an excellent stability of over 60 days. The AuNPs showed broad-spectrum antibacterial activities against both pathogenic Gram-positive (Staphylococcus epidermidis (ATCC14990), Staphylococcus aureus (ATCC 25923), Mycobacterium smegmatis (MC 215)) and Gram-negative bacteria (Proteus mirabilis (ATCC 7002), Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 13822), Klebsiella oxytoca (ATCC 8724)), with a minimum inhibition concentration of 62.5 µg/mL. In addition, the as-synthesized AuNPs were highly stable with exceptional cell viability towards normal cells (BHK- 21) and cancerous cancer cell lines (cervical and lung cancer).

scholarly journals Kaolin based protective barrier in municipal landfills against adverse chemo-mechanical loadings

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Partha Das ◽  
Tadikonda Venkata Bharat
Keyword(s):  
Ionic Strength ◽  
High Ionic Strength ◽  
The Self ◽  
Design Parameters ◽  
Layered System ◽  
Geosynthetic Clay Liners ◽  
Clay Liners ◽  
Landfill Liner ◽  
First Time ◽  
Applied Stresses

AbstractIn this work, we assess the self-sealing and swelling ability of the compacted granular bentonite (GB) under an inorganic salt environment and induced overburden stresses from the landfill waste. The laboratory permeation tests with high ionic strength salt solutions reveal that the GB fails to seal and exhibits a significant mechanical collapse under different applied stresses. The applicability of GB in the form of geosynthetic clay liners as the bottom liner facilities in landfills that produce high ionic strength salt leachates, therefore, remains a serious concern. We propose an additional barrier system based on kaolin, for the first time, to address this problem. The proposed kaolin-GB layered system performs satisfactorily in terms of its sealing and swelling ability even in adverse saline conditions and low overburden stresses. The kaolin improves the osmotic efficiency of the self and also helps the underlying GB layer to seal the inter-granular voids. The estimated design parameters by through-diffusion test suggest that the kaolin-GB layered system effectively attenuates the permeant flux and suitable as a landfill liner.

Enhancement of efficiency in graphene/porous silicon solar cells by co-doping graphene with gold nanoparticles and bis(trifluoromethanesulfonyl)-amide

2017 ◽  
Vol 5 (35) ◽  
pp. 9005-9011 ◽  
Author(s):  
Ju Hwan Kim ◽  
Dong Hee Shin ◽  
Ha Seung Lee ◽  
Chan Wook Jang ◽  
Jong Min Kim ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Solar Cells ◽  
Porous Silicon ◽  
Au Nanoparticles ◽  
Silicon Solar Cells ◽  
Porous Si ◽  
Co Doping ◽  
Si Solar Cells ◽  
First Time

The co-doping of graphene with Au nanoparticles and bis(trifluoromethanesulfonyl)-amide is employed for the first time to enhance the performance of graphene/porous Si solar cells.

The Langmuir–Hinshelwood approach for kinetic evaluation of cucurbit[7]uril-capped gold nanoparticles in the reduction of the antimicrobial nitrofurantoin

2017 ◽  
Vol 19 (29) ◽  
pp. 18913-18923 ◽  
Author(s):  
E. Blanco ◽  
P. Atienzar ◽  
P. Hernández ◽  
C. Quintana
Keyword(s):  
Gold Nanoparticles ◽  
Nitro Compound ◽  
Kinetic Constants ◽  
Kinetic Evaluation ◽  
P Adsorption ◽  
First Time

Adsorption and surface kinetic constants of nitrofurantoin are calculated and compared with those of another nitro compound for the first time.

Gold nanoparticles immobilised in a superabsorbent hydrogel matrix: facile synthesis and application for the catalytic reduction of toxic compounds

2020 ◽  
Vol 56 (8) ◽  
pp. 1263-1266 ◽  
Author(s):  
Hassan A. H. Alzahrani ◽  
Mark A. Buckingham ◽  
William P. Wardley ◽  
Richard D. Tilley ◽  
Nicholas Ariotti ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Ionic Strength ◽  
Catalytic Reduction ◽  
High Ionic Strength ◽  
Facile Synthesis ◽  
Superabsorbent Hydrogel ◽  
Toxic Compounds ◽  
Hydrogel Matrix ◽  
Catalytically Active

AuNP easily synthesised inside bulk hydrogel spheres; stable and catalytically active, even in high ionic strength environments.

Layer-by-layer self-assembly of polydopamine/gold nanoparticle/thiol coating as the stationary phase for open tubular capillary electrochromatography

2015 ◽  
Vol 7 (19) ◽  
pp. 8227-8234 ◽  
Author(s):  
Xiu-Ju Li ◽  
Qi-Feng Fu ◽  
Qi-Hui Zhang ◽  
Xue-Mei Jiang ◽  
Feng-Qing Yang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Stationary Phase ◽  
Gold Nanoparticle ◽  
Self Assembly ◽  
Capillary Electrochromatography ◽  
Phase Ratio ◽  
Layer By Layer ◽  
Novel Method ◽  
First Time ◽  
High Phase

A novel method for the preparation of permanent coating columns with a high phase ratio based on the multiple properties of PDA and LBL self-assembly of polydopamine/gold nanoparticles/thiols has been developed for the first time.

Evaluation of a New Automatic Calcium Ion Analyzer

1977 ◽  
Vol 23 (4) ◽  
pp. 690-694 ◽  
Author(s):  
SØren Madsen ◽  
Klaus ⵁlgaard
Keyword(s):  
Fluid Flow ◽  
Ionic Strength ◽  
Calcium Ion ◽  
Calcium Binding ◽  
Routine Laboratory ◽  
Mean Values ◽  
Binding Effect ◽  
Physiological Range ◽  
Flow Through ◽  
First Time

Abstract We evaluated a new automatic analyzer for ionized cal¬cium (Ca2+), the Orion Model SS-20, based on a flow-through ion-exchange electrode. Ca2+ was measured in heparinized whole blood and serum. Within-day variation was 1.2%, day-to-day variation 1.6 %, and analytical re-covery 92.4%. Over the physiological range interference by K± and Mg2+ was negligible; major changes in ionic strength, induced by changes in Na+ concentration, made correction for a sodium error necessary. Within the physiological range, Ca2+ was inversely correlated to variations in pH. Therefore, to compare Ca2+ values, correction to an apparent pH of 7.40 should be made. The calcium binding effect of heparin was negligible when minimal (4.4 int. units/m1) concentrations of heparin were used. Storage of serum at 4 °C for one week resulted in a 4% decrease in apparent serum Ca2+, primarily owing to an increase in pH during storage. In normal material, mean values for blood- and serum-Ca2+ (1.10 and 1.07 mmol/liter, respectively) were close to results obtained by previous systems. Errors caused by disturbances in the fluid flow and non-function of half the electrodes we re¬ceived were the major inconveniences of the analyzer. We conclude that this new analyzer gives decisive advantages in measurement of Ca2+, making this important analysis possible as a routine laboratory test for the first time

Preparation of Highly Stable Oligo(ethylene glycol) Derivatives-Functionalized Gold Nanoparticles and Their Application in LSPR-Based Detection of PSA/ACT Complex

2007 ◽  
Vol 7 (11) ◽  
pp. 3754-3757 ◽  
Author(s):  
Cuong Cao ◽  
Sang Jun Sim
Keyword(s):  
Gold Nanoparticles ◽  
Colloidal Particles ◽  
Sandwich Immunoassay ◽  
Immunoassay Technique ◽  
Gold Nanoparticle Aggregation ◽  
Wide Ph Range ◽  
Complex Detection ◽  
Ethylene Glycol Derivatives ◽  
Ph Range ◽  
First Time

A sandwich immunoassay for PSA/ACT complex detection based on gold nanoparticle aggregation using two probes was developed. The functionalized colloidal gold nanoparticles (AuNPs) showed highly stable not only in the presence of high ionic strength but also in a wide pH range. The functionalized AuNPs were tagged with PSA/ACT complex monoclonal antibody and goat PSA polyclonal antibody and served as the probes to induce aggregation of the colloidal particles. As a result, PSA/ACT complex was detected at concentrations as low as 1 ng/ml. This is the first time that a new aggregation sandwich-immunoassay technique using two gold probes has been used, and the results are generally applicable to other LSPR-based immunoassays.

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