Pencil graphite as an elegant electrochemical sensor for separation-free and simultaneous sensing of hypoxanthine, xanthine and uric acid in fish samples

2017 ◽  
Vol 9 (15) ◽  
pp. 2265-2274 ◽  
Author(s):  
Nandimalla Vishnu ◽  
Mansi Gandhi ◽  
Desikan Rajagopal ◽  
Annamalai Senthil Kumar

Herein, we report a simple electroanalytical detection method based on ultra-low cost pencil graphite as an elegant sensor for simultaneous and separation-free detection of Hx, X and UA.

RSC Advances ◽  
2016 ◽  
Vol 6 (79) ◽  
pp. 75741-75748 ◽  
Author(s):  
S. Jesny ◽  
Shalini Menon ◽  
K. Girish Kumar

Simultaneous sensing of guanine and adenine in presence of uric acid in alkaline medium by polymer modified electrode.


2019 ◽  
Vol 15 (6) ◽  
pp. 628-634
Author(s):  
Rong Liu ◽  
Jie Li ◽  
Tongsheng Zhong ◽  
Liping Long

Background: The unnatural levels of dopamine (DA) result in serious neurological disorders such as Parkinson’s disease. Electrochemical methods which have the obvious advantages of simple operation and low-cost instrumentation were widely used for determination of DA. In order to improve the measurement performance of the electrochemical sensor, molecular imprinting technique and graphene have always been employed to increase the selectivity and sensitivity. Methods: An electrochemical sensor which has specific selectivity to (DA) was proposed based on the combination of a molecular imprinting polymer (MIP) with a graphene (GR) modified gold electrode. The performance and effect of MIP film were investigated by differential pulse voltammetry (DPV) and cyclic voltammetry (CV) in the solution of 5.0 ×10-3 mol/L K3[Fe(CN)6] and K4[Fe(CN)6] with 0.2 mol/L KCl at room temperature. Results: This fabricated sensor has well repeatability and stability, and was used to determine the dopamine of urine. Under the optimized experiment conditions, the current response of the imprinted sensor was linear to the concentration of dopamine in the range of 1.0×10-7 ~ 1.0×10-5 mol/L, the linear equation was I (µA) = 7.9824+2.7210lgc (mol/L) with the detection limit of 3.3×10-8 mol/L. Conclusion: In this work, a highly efficient sensor for determination of DA was prepared with good sensitivity by GR and great selectivity of high special recognization ability by molecular imprinting membrane. This proposed sensor was used to determine the dopamine in human urine successfully.


Materials ◽  
2020 ◽  
Vol 14 (1) ◽  
pp. 37
Author(s):  
Mayra K. S. Monteiro ◽  
Djalma R. Da Silva ◽  
Marco A. Quiroz ◽  
Vítor J. P. Vilar ◽  
Carlos A. Martínez-Huitle ◽  
...  

This study aims to investigate the applicability of a hybrid electrochemical sensor composed of cork and graphite (Gr) for detecting caffeine in aqueous solutions. Raw cork (RAC) and regranulated cork (RGC, obtained by thermal treatment of RAC with steam at 380 °C) were tested as modifiers. The results clearly showed that the cork-graphite sensors, GrRAC and GrRGC, exhibited a linear response over a wide range of caffeine concentration (5–1000 µM), with R2 of 0.99 and 0.98, respectively. The limits of detection (LOD), estimated at 2.9 and 6.1 µM for GrRAC and GrRGC, suggest greater sensitivity and reproducibility than the unmodified conventional graphite sensor. The low-cost cork-graphite sensors were successfully applied in the determination of caffeine in soft drinks and pharmaceutical formulations, presenting well-defined current signals when analyzing real samples. When comparing electrochemical determinations and high performance liquid chromatography measurements, no significant differences were observed (mean accuracy 3.0%), highlighting the potential use of these sensors to determine caffeine in different samples.


Biosensors ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 85
Author(s):  
Wassa Waiwinya ◽  
Thitirat Putnin ◽  
Dechnarong Pimalai ◽  
Wireeya Chawjiraphan ◽  
Nuankanya Sathirapongsasuti ◽  
...  

An immobilization-free electrochemical sensor coupled with a graphene oxide (GO)-based aptasensor was developed for glycated human serum albumin (GHSA) detection. The concentration of GHSA was monitored by measuring the electrochemical response of free GO and aptamer-bound GO in the presence of glycated albumin; their currents served as the analytical signals. The electrochemical aptasensor exhibited good performance with a base-10 logarithmic scale. The calibration curve was achieved in the range of 0.01–50 µg/mL. The limit of detection (LOD) was 8.70 ng/mL. The developed method was considered a one-drop measurement process because a fabrication step and the probe-immobilization process were not required. This simple sensor offers a cost-effective, rapid, and sensitive detection method, and could be an alternative approach for determination of GHSA levels.


Symmetry ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 678
Author(s):  
Vladimir Tadic ◽  
Tatjana Loncar-Turukalo ◽  
Akos Odry ◽  
Zeljen Trpovski ◽  
Attila Toth ◽  
...  

This note presents a fuzzy optimization of Gabor filter-based object and text detection. The derivation of a 2D Gabor filter and the guidelines for the fuzzification of the filter parameters are described. The fuzzy Gabor filter proved to be a robust text an object detection method in low-quality input images as extensively evaluated in the problem of license plate localization. The extended set of examples confirmed that the fuzzy optimized Gabor filter with adequately fuzzified parameters detected the desired license plate texture components and highly improved the object detection when compared to the classic Gabor filter. The robustness of the proposed approach was further demonstrated on other images of various origin containing text and different textures, captured using low-cost or modest quality acquisition procedures. The possibility to fine tune the fuzzification procedure to better suit certain applications offers the potential to further boost detection performance.


The Analyst ◽  
2021 ◽  
Author(s):  
Jinyuan Chen ◽  
Hongxiang Wei ◽  
Xinyu Fang ◽  
Yuanqing Cai ◽  
Zhenzhen Zhang ◽  
...  

A pragmatic electrochemical mecA gene detection method for a rapid, accurate and low-cost identification of MRSA in the joint synovial fluid of PJI patients.


2017 ◽  
Vol 799 ◽  
pp. 459-467 ◽  
Author(s):  
Qing Zhu ◽  
Jing Bao ◽  
Danqun Huo ◽  
Mei Yang ◽  
Huixiang Wu ◽  
...  

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