Three-dimensional microtissues as an in vitro model for personalized radiation therapy

The Analyst ◽  
2017 ◽  
Vol 142 (19) ◽  
pp. 3605-3612 ◽  
Author(s):  
Yuting Qiu ◽  
Dandan Ning ◽  
Peipei Zhang ◽  
Stephanie Curly ◽  
Yong Qiao ◽  
...  
Keyword(s):  
Cell Culture ◽  
Dna Damage ◽  
Animal Model ◽  
Radiation Therapy ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Intermediate Model ◽  
Radiation Induced ◽  
Vitro Model

This paper describes the use of 3D microtissues as an intermediate model between the 2D cell culture and the animal model to assess radiation-induced cellular and DNA damage in the context of personalized radiation therapy.

scholarly journals A three-dimensional in vitro model of the peripheral nervous system

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Sunghee Estelle Park ◽  
Jinchul Ahn ◽  
Hyo-Eun Jeong ◽  
Inchan Youn ◽  
Dongeun Huh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Nervous System ◽  
Peripheral Nervous System ◽  
In Vitro Model ◽  
Three Dimensional ◽  
3D Cell Culture ◽  
Nerve Fibers ◽  
Coculture Model ◽  
Vitro Model

AbstractRecent advances in three-dimensional (3D) cell culture models developed on organ-on-a-chip or microfluidic devices have shown their capability to recapitulate the in vivo microenvironment as well as their potential as tools in biomedical research. Here, we present an in vitro model of the peripheral nervous system (PNS) by establishing a coculture model of motor neurons (MNs) and Schwann cells (SCs) in a 3D environment in a microengineered extracellular matrix hydrogel scaffold. The collagen scaffold placed at the center of the microdevice provided a 3D cellular microenvironment where the axons of MNs were allowed to actively interact with SCs during their growth and maturation. By treating the MN–SC coculture model with ascorbic acid, we were able to model the myelination process in the PNS, which was evidenced by the increased expression of myelin markers in SCs. Moreover, we show that this can be reversed by treating myelinated nerve fibers with glial growth factor (neuregulin-1 isoform) to potentially block the formation of the myelin sheath and induce demyelination. Our 3D cell culture model may be used to achieve active control of the myelinating and demyelinating processes in the PNS and thus may offer new opportunities to study pathophysiological processes involved in motor neuron diseases by in vitro modeling.

scholarly journals Crocetin Mitigates Irradiation Injury in an In Vitro Model of the Pubertal Testis: Focus on Biological Effects and Molecular Mechanisms

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1676
Author(s):  
Giulia Rossi ◽  
Martina Placidi ◽  
Chiara Castellini ◽  
Francesco Rea ◽  
Settimio D'Andrea ◽  
...  
Keyword(s):  
In Vitro Model ◽  
Molecular Mechanisms ◽  
Biological Effects ◽  
Cellular Damage ◽  
X Rays ◽  
Adolescent Cancer ◽  
Radiation Induced ◽  
Vitro Model ◽  
Underlying Mechanisms

Infertility is a potential side effect of radiotherapy and significantly affects the quality of life for adolescent cancer survivors. Very few studies have addressed in pubertal models the mechanistic events that could be targeted to provide protection from gonadotoxicity and data on potential radioprotective treatments in this peculiar period of life are elusive. In this study, we utilized an in vitro model of the mouse pubertal testis to investigate the efficacy of crocetin to counteract ionizing radiation (IR)-induced injury and potential underlying mechanisms. Present experiments provide evidence that exposure of testis fragments from pubertal mice to 2 Gy X-rays induced extensive structural and cellular damage associated with overexpression of PARP1, PCNA, SOD2 and HuR and decreased levels of SIRT1 and catalase. A twenty-four hr exposure to 50 μM crocetin pre- and post-IR significantly reduced testis injury and modulated the response to DNA damage and oxidative stress. Nevertheless, crocetin treatment did not counteract the radiation-induced changes in the expression of SIRT1, p62 and LC3II. These results increase the knowledge of mechanisms underlying radiation damage in pubertal testis and establish the use of crocetin as a fertoprotective agent against IR deleterious effects in pubertal period.

scholarly journals Skeletal muscle-on-a-chip: an in vitro model to evaluate tissue formation and injury

Lab on a Chip ◽  
2017 ◽  
Vol 17 (20) ◽  
pp. 3447-3461 ◽  
Author(s):  
Gaurav Agrawal ◽  
Aereas Aung ◽  
Shyni Varghese
Keyword(s):  
Skeletal Muscle ◽  
Muscle Injury ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Tissue Formation ◽  
Microfluidic Platform ◽  
Muscle Tissues ◽  
Vitro Model

We introduce a microfluidic platform in which we culture three-dimensional skeletal muscle tissues, while evaluating tissue formation and toxin-induced muscle injury.

Characterization of secretomes from a human blood brain barrier endothelial cells in-vitro model after ischemia by stable isotope labeling with aminoacids in cell culture (SILAC)

2016 ◽  
Vol 133 ◽  
pp. 100-112 ◽  
Author(s):  
Victor Llombart ◽  
Teresa García-Berrocoso ◽  
Joan Josep Bech-Serra ◽  
Alba Simats ◽  
Alejandro Bustamante ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cells ◽  
Stable Isotope ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Isotope Labeling ◽  
Vitro Model

SU-FF-J-104: In Vitro Model to Study the Biological Effect of Dose Gradients On a Cell Culture System

2005 ◽  
Vol 32 (6Part6) ◽  
pp. 1944-1944 ◽  
Author(s):  
R Bromley ◽  
L Oliver ◽  
R Harvie ◽  
R Davey
Keyword(s):  
Cell Culture ◽  
Biological Effect ◽  
Culture System ◽  
In Vitro Model ◽  
Cell Culture System ◽  
A Cell ◽  
Vitro Model

scholarly journals A three-dimensional in vitro model of erythropoiesis recapitulates erythroid failure in myelodysplastic syndromes

Leukemia ◽  
2019 ◽  
Vol 34 (1) ◽  
pp. 271-282 ◽  
Author(s):  
Edda María Elvarsdóttir ◽  
Teresa Mortera-Blanco ◽  
Marios Dimitriou ◽  
Thibault Bouderlique ◽  
Monika Jansson ◽  
...  
Keyword(s):  
Myelodysplastic Syndromes ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Vitro Model

scholarly journals The efficacy of cetuximab in a tissue-engineered three-dimensional in vitro model of colorectal cancer

2014 ◽  
Vol 5 ◽  
pp. 204173141454418 ◽  
Author(s):  
Tarig Magdeldin ◽  
Víctor López-Dávila ◽  
Cecile Villemant ◽  
Grant Cameron ◽  
Rosemary Drake ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
In Vitro Model ◽  
Three Dimensional ◽  
Vitro Model
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