In vivo endoscopic mass spectrometry using a moving string sampling probe

The Analyst ◽  
2017 ◽  
Vol 142 (15) ◽  
pp. 2735-2740 ◽  
Author(s):  
Lee Chuin Chen ◽  
Tsubasa Naito ◽  
Satoru Tsutsui ◽  
Yuki Yamada ◽  
Satoshi Ninomiya ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Transportation System ◽  
Sampling Probe

A novel moving string sampling probe and sample transportation system for performing in situ and in vivo endoscopic MS.

Biocompatible Surface-Coated Probe for in Vivo, in Situ, and Microscale Lipidomics of Small Biological Organisms and Cells Using Mass Spectrometry

2018 ◽  
Vol 90 (11) ◽  
pp. 6936-6944 ◽  
Author(s):  
Jiewei Deng ◽  
Wenying Li ◽  
Qiuxia Yang ◽  
Yaohui Liu ◽  
Ling Fang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biological Organisms

scholarly journals Transforming presumptive forensic testing: in situ identification and age estimation of human bodily fluids

2019 ◽  
Vol 10 (4) ◽  
pp. 1064-1069 ◽  
Author(s):  
Stephanie Rankin-Turner ◽  
Matthew A. Turner ◽  
Paul F. Kelly ◽  
Roberto S. P. King ◽  
James C. Reynolds
Keyword(s):  
Mass Spectrometry ◽  
Age Estimation ◽  
Rapid Identification ◽  
New Method ◽  
Bodily Fluids ◽  
Sampling Probe ◽  
Forensic Testing

A new method utilising an in situ sampling probe coupled with mass spectrometry for rapid identification and age estimation of biofluids.

In Vivo, In Situ Tissue Analysis Using Rapid Evaporative Ionization Mass Spectrometry

2009 ◽  
Vol 48 (44) ◽  
pp. 8240-8242 ◽  
Author(s):  
Karl-Christian Schäfer ◽  
Júlia Dénes ◽  
Katalin Albrecht ◽  
Tamás Szaniszló ◽  
Júlia Balog ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Tissue Analysis

scholarly journals Mass spectrometry imaging of the in situ drug release from nanocarriers

2018 ◽  
Vol 4 (10) ◽  
pp. eaat9039 ◽  
Author(s):  
Jinjuan Xue ◽  
Huihui Liu ◽  
Suming Chen ◽  
Caiqiao Xiong ◽  
Lingpeng Zhan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Delivery ◽  
Drug Release ◽  
Drug Carrier ◽  
Ionization Mass ◽  
Label Free ◽  
Normal Tissues ◽  
Liver Tissues

It is crucial but of a great challenge to study in vivo and in situ drug release of nanocarriers when developing a nanomaterial-based drug delivery platform. We developed a new label-free laser desorption/ionization mass spectrometry (MS) imaging strategy that enabled visualization and quantification of the in situ drug release in tissues by monitoring intrinsic MS signal intensity ratio of loaded drug over the nanocarriers. The proof of concept was demonstrated by investigating the doxorubicin (DOX)/polyethylene glycol–MoS2 nanosheets drug delivery system in tumor mouse models. The results revealed a tissue-dependent release behavior of DOX during circulation with the highest dissociation in tumor and lowest dissociation in liver tissues. The drug-loaded MoS2 nanocarriers are predominantly distributed in lung, spleen, and liver tissues, whereas the accumulation in the tumor was unexpectedly lower than in normal tissues. This new strategy could also be extended to other drug-carrier systems, such as carbon nanotubes and black phosphorus nanosheets, and opened a new path to evaluate the drug release of nanocarriers in the suborgan level.

scholarly journals Highly effective proximate labeling in Drosophila

2021 ◽  
Author(s):  
Bo Zhang ◽  
Yuanbing Zhang ◽  
Ji-Long Liu
Keyword(s):  
Mass Spectrometry ◽  
Developmental Stages ◽  
Cell Types ◽  
Good Resolution ◽  
Spatiotemporal Resolution ◽  
Protein Protein Interaction ◽  
Ppi Networks ◽  
Wide Range

Abstract The protein-protein interaction (PPI) is a basic strategy for life to operate. The analysis of PPIs in multicellular organisms is very important but extremely challenging because PPIs are particularly dynamic and variable among different development stages, tissues, cells, and even organelles. Therefore, understanding PPI needs a good resolution of time and space. More importantly, understanding in vivo PPI needs to be realized in situ. Proximity-based biotinylation combined with mass spectrometry has emerged as a powerful approach to study PPI networks and protein subcellular compartmentation. TurboID, the newly engineered promiscuous ligase, has been reported to label bait proteins effectively in various species. In Drosophila, we systematically apply TurboID-mediated biotinylation in a wide range of developmental stages and tissues, and demonstrate the feasibility of TurboID-mediated labeling system in desired cell types. For a proof-of-principle, we use the TurboID-mediated biotinylation coupled with mass spectrometry to distinguish CTP synthase with or without the ability to form filamentous cytoophidia, retrieving two distinct sets of proximate proteomes. Therefore, this makes it possible to map PPIs in vivo and in situ at a defined spatiotemporal resolution, and demonstrates a referable resource for cytoophidium proteome in Drosophila.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

Three-Dimensional Subcellular Organization of Hepatocytes in Intact Liver: Simultaneous Visualization of Cytoskeletal and Membrane Markers by Confocal Microscopy

1996 ◽  
Vol 54 ◽  
pp. 288-289
Author(s):  
Greg V. Martin ◽  
Ann L. Hubbard
Keyword(s):  
Three Dimensional ◽  
Integral Membrane Proteins ◽  
Polarized Secretion ◽  
Microscope Images ◽  
Computer Aided ◽  
Intracellular Organelles ◽  
Cytoskeletal Elements ◽  
Simultaneous Visualization

The microtubule (MT) cytoskeleton is necessary for many of the polarized functions of hepatocytes. Among the functions dependent on the MT-based cytoskeleton are polarized secretion of proteins, delivery of endocytosed material to lysosomes, and transcytosis of integral plasma membrane (PM) proteins. Although microtubules have been shown to be crucial to the establishment and maintenance of functional and structural polarization in the hepatocyte, little is known about the architecture of the hepatocyte MT cytoskeleton in vivo, particularly with regard to its relationship to PM domains and membranous organelles. Using an in situ extraction technique that preserves both microtubules and cellular membranes, we have developed a protocol for immunofluorescent co-localization of cytoskeletal elements and integral membrane proteins within 20 µm cryosections of fixed rat liver. Computer-aided 3D reconstruction of multi-spectral confocal microscope images was used to visualize the spatial relationships among the MT cytoskeleton, PM domains and intracellular organelles.

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