Ambient mass spectrometry for in vivo local analysis and in situ molecular tissue imaging

2012 ◽  
Vol 34 ◽  
pp. 22-34 ◽  
Author(s):  
Peter Nemes ◽  
Akos Vertes
Keyword(s):  
Mass Spectrometry ◽  
Ambient Mass Spectrometry ◽  
Tissue Imaging ◽  
Local Analysis

Biocompatible Surface-Coated Probe for in Vivo, in Situ, and Microscale Lipidomics of Small Biological Organisms and Cells Using Mass Spectrometry

2018 ◽  
Vol 90 (11) ◽  
pp. 6936-6944 ◽  
Author(s):  
Jiewei Deng ◽  
Wenying Li ◽  
Qiuxia Yang ◽  
Yaohui Liu ◽  
Ling Fang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biological Organisms

In vivo endoscopic mass spectrometry using a moving string sampling probe

The Analyst ◽  
2017 ◽  
Vol 142 (15) ◽  
pp. 2735-2740 ◽  
Author(s):  
Lee Chuin Chen ◽  
Tsubasa Naito ◽  
Satoru Tsutsui ◽  
Yuki Yamada ◽  
Satoshi Ninomiya ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Transportation System ◽  
Sampling Probe

A novel moving string sampling probe and sample transportation system for performing in situ and in vivo endoscopic MS.

In Situ Hydrogel Conditioning of Tissue Samples To Enhance the Drug’s Sensitivity in Ambient Mass Spectrometry Imaging

2017 ◽  
Vol 89 (12) ◽  
pp. 6318-6323 ◽  
Author(s):  
Xiaowei Song ◽  
Zhigang Luo ◽  
Xin Li ◽  
Tiegang Li ◽  
Zhonghua Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Ambient Mass Spectrometry ◽  
Tissue Samples ◽  
In Situ Hydrogel

Distribution, function, and prognostic value of cytotoxic T lymphocytes in follicular lymphoma: a 3-D tissue-imaging study

Blood ◽  
2011 ◽  
Vol 118 (20) ◽  
pp. 5371-5379 ◽  
Author(s):  
Camille Laurent ◽  
Sabina Müller ◽  
Catherine Do ◽  
Talal Al-Saati ◽  
Sophie Allart ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Lymph Nodes ◽  
Follicular Lymphoma ◽  
Imaging Study ◽  
Progression Free Survival ◽  
Tissue Imaging ◽  
Combined Chemotherapy ◽  
3 Dimensional

Abstract CD8+ CTLs are thought to play a role in the control of follicular lymphoma (FL). Yet, the link between CTL tissue distribution, activation status, ability to kill FL cells in vivo, and disease progression is still elusive. Pretreatment lymph nodes from FL patients were analyzed by IHC (n = 80) or by 3-color confocal microscopy (n = 10). IHC revealed a rich infiltrate of CD8+ granzyme B+ (GrzB) cells in FL interfollicular spaces. Accordingly, confocal microscopy showed an increased number of CD3+CD8+GrzB+ CTLs and a brighter GrzB staining in individual CTL in FL samples compared with reactive lymph nodes. CTLs did not penetrate tumor nodules. In 3-dimensional (3-D) image reconstructions, CTLs were detected at the FL follicle border where they formed lytic synapse-like structures with FL B cells and with apoptotic cells, suggesting an in situ cytotoxic function. Finally, although GrzB expression in CTLs did not correlate with risk factors, high GrzB content correlated with prolonged progression free-survival (PFS) after rituximab-combined chemotherapy. Our results show the recruitment of armed CTLs with a tumor-controlling potential into FL lymph nodes and suggest that CTL-associated GrzB expression could influence PFS in FL patients having received rituximab-combined chemotherapy.

Rapid In Situ Profiling of Lipid C═C Location Isomers in Tissue Using Ambient Mass Spectrometry with Photochemical Reactions

2018 ◽  
Vol 90 (9) ◽  
pp. 5612-5619 ◽  
Author(s):  
Fei Tang ◽  
Chengan Guo ◽  
Xiaoxiao Ma ◽  
Jian Zhang ◽  
Yuan Su ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Photochemical Reactions ◽  
Ambient Mass Spectrometry

In Vivo, In Situ Tissue Analysis Using Rapid Evaporative Ionization Mass Spectrometry

2009 ◽  
Vol 48 (44) ◽  
pp. 8240-8242 ◽  
Author(s):  
Karl-Christian Schäfer ◽  
Júlia Dénes ◽  
Katalin Albrecht ◽  
Tamás Szaniszló ◽  
Júlia Balog ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Tissue Analysis

scholarly journals Recent Advances of Ambient Mass Spectrometry Imaging and Its Applications in Lipid and Metabolite Analysis

Metabolites ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 780
Author(s):  
Keke Qi ◽  
Liutian Wu ◽  
Chengyuan Liu ◽  
Yang Pan
Keyword(s):  
Mass Spectrometry ◽  
Mass Spectrometry Imaging ◽  
Disease Diagnosis ◽  
Ambient Mass Spectrometry ◽  
Ambient Conditions ◽  
Metabolite Analysis ◽  
Tissue Sections ◽  
Model Research ◽  
One Step

Ambient mass spectrometry imaging (AMSI) has attracted much attention in recent years. As a kind of unlabeled molecular imaging technique, AMSI can enable in situ visualization of a large number of compounds in biological tissue sections in ambient conditions. In this review, the developments of various AMSI techniques are discussed according to one-step and two-step ionization strategies. In addition, recent applications of AMSI for lipid and metabolite analysis (from 2016 to 2021) in disease diagnosis, animal model research, plant science, drug metabolism and toxicology research, etc., are summarized. Finally, further perspectives of AMSI in spatial resolution, sensitivity, quantitative ability, convenience and software development are proposed.

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