Immunoassay quantification using surface-enhanced fluorescence (SEF) tags

The Analyst ◽  
2017 ◽  
Vol 142 (15) ◽  
pp. 2717-2724 ◽  
Author(s):  
S. A. Camacho ◽  
R. G. Sobral-Filho ◽  
P. H. B. Aoki ◽  
C. J. L. Constantino ◽  
A. G. Brolo
Keyword(s):  
Organic Dyes ◽  
Fluorescence Immunoassay ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Surface Enhanced

A fluorescence immunoassay based on surface-enhanced fluorescence (SEF) was introduced. The use of SEF tags may provide advantages over regular organic dyes for biomedical assays.

scholarly journals A straightforward and sensitive “ON–OFF” fluorescence immunoassay based on silicon-assisted surface enhanced fluorescence

RSC Advances ◽  
2021 ◽  
Vol 11 (13) ◽  
pp. 7723-7731
Author(s):  
Ruohu Zhang ◽  
Zhanrui Jin ◽  
Zhengqiu Tian ◽  
Yingzhou Liu ◽  
Zhengqi Lu ◽  
...  
Keyword(s):  
Fluorescence Immunoassay ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Surface Enhanced ◽  
Silicon Based

A straightforward immunoassay based on silicon-assisted surface enhanced fluorescence (SEF) has been demonstrated using a silicon-based fluorescent immune substrate and silver-antibody nanoconjugate (SANC).

scholarly journals Surface enhanced fluorescence of Ag nanostructure films prepared by DC magnetron sputtering

2020 ◽  
Author(s):  
V. Atimayulerd ◽  
N. Srisuai ◽  
S. Boonruang ◽  
S. Kalasung ◽  
C. Chananonnawathorn ◽  
...  
Keyword(s):  
Magnetron Sputtering ◽  
Dc Magnetron Sputtering ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Surface Enhanced

Study on surface enhanced fluorescence based on Ag@razor clam substrate

2021 ◽  
Vol 488 ◽  
pp. 126863
Author(s):  
Hongwen Cao ◽  
Zhen Sun ◽  
Liting Guo ◽  
Na Li ◽  
Zubin Shang ◽  
...  
Keyword(s):  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Razor Clam ◽  
Surface Enhanced

scholarly journals Application of AAO Matrix in Aligned Gold Nanorod Array Substrates for Surface-Enhanced Fluorescence and Raman Scattering

Plasmonics ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. 1371-1376 ◽  
Author(s):  
Signe Damm ◽  
Frances Lordan ◽  
Antony Murphy ◽  
Mark McMillen ◽  
Robert Pollard ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Nanorod Array ◽  
Gold Nanorod ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Surface Enhanced

High Contrast Surface Enhanced Fluorescence of Carbon Dot Labeled Bacteria Cells on Aluminum Foil

2020 ◽  
Vol 30 (6) ◽  
pp. 1477-1482 ◽  
Author(s):  
Rostislav Bukasov ◽  
Zhanar Kunushpayeva ◽  
Alisher Rapikov ◽  
Saida Zhunussova ◽  
Alisher Sultangaziyev ◽  
...  
Keyword(s):  
Aluminum Foil ◽  
High Contrast ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Carbon Dot ◽  
Surface Enhanced

scholarly journals Monitoring individual cell-signaling activity using combined metal-clad waveguide and surface-enhanced fluorescence imaging

The Analyst ◽  
2018 ◽  
Vol 143 (22) ◽  
pp. 5559-5567 ◽  
Author(s):  
Thomas Söllradl ◽  
Kevin Chabot ◽  
Ulrike Fröhlich ◽  
Michael Canva ◽  
Paul G. Charette ◽  
...  
Keyword(s):  
Cell Signaling ◽  
Fluorescence Imaging ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Individual Cell ◽  
Live Cell ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Surface Enhanced ◽  
Signaling Activity

Validation of a combined metal-clad waveguide and surface enhanced fluorescence imaging platform for live cell imaging.

scholarly journals Magnetron-Sputtered Polytetrafluoroethylene-Stabilized Silver Nanoisland Surface for Surface-Enhanced Fluorescence

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 773 ◽  
Author(s):  
Martin Šubr ◽  
Petr Praus ◽  
Anna Kuzminova ◽  
Eva Kočišová ◽  
Ondřej Kylián ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Radiative Decay ◽  
Adsorbed Layer ◽  
Localized Surface Plasmon ◽  
Homogeneous Layer ◽  
Excitation Wavelength ◽  
Sessile Droplet ◽  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Surface Enhanced

Surface-enhanced fluorescence (SEF) requires the absorption/emission band of the fluorophore, the localized surface plasmon resonance (LSPR) of the nanostructure and the excitation wavelength to fall in the same (or very close) spectral range. In this paper, we monitor the SEF intensity and lifetime dependence of riboflavin (vitamin B2) adsorbed on a spacer-modified Ag substrate with respect to the thickness of the spacer. The substrates were formed by silver nanoislands deposited onto magnetron-sputtered polytetrafluoroethylene (ms-PTFE). The spacer was formed by the ms-PTFE layer with the thickness ranging from ~5 to 25 nm. The riboflavin dissolved in dimethylsulfoxide (DMSO) at a 10 µM concentration forms, at the ms-PTFE surface, a homogeneous layer of adsorbed molecules corresponding to a monomolecular layer. The microspectroscopic measurements of the adsorbed layer were performed through a sessile droplet; our study has shown the advantages and limitations of this approach. Time-resolved fluorescence enabled us to determine the enhanced fluorescence quantum yield due to the shortening of the radiative decay in the vicinity of the plasmonic surface. For the 5 nm ms-PTFE layer possessing the largest (estimated 4×) fluorescence enhancement, the quantum yield was increased 2.3×.

Surface-Enhanced Fluorescence on SiO2-Coated Silver Island Films

1999 ◽  
Vol 53 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Peter J. Tarcha ◽  
J. Desaja-Gonzalez ◽  
S. Rodriguez-Llorente ◽  
R. Aroca
Keyword(s):  
Enhanced Fluorescence ◽  
Surface Enhanced Fluorescence ◽  
Silver Island Films ◽  
Surface Enhanced ◽  
Silver Island ◽  
Island Films
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