K-junction structure mediated exponential signal amplification strategy for microRNA detection in electrochemiluminescence biosensor

The Analyst ◽  
2017 ◽  
Vol 142 (12) ◽  
pp. 2185-2190 ◽  
Author(s):  
Xue Li ◽  
Anyi Chen ◽  
Yaqin Chai ◽  
Ruo Yuan
Keyword(s):  
Signal Amplification ◽  
The Novel ◽  
Microrna Detection ◽  
G Quadruplex ◽  
Simple Protocol ◽  
Amplification Strategy ◽  
Junction Structure

Based on the novel designed K-junction structure, an economic and efficient exponential signal amplification strategy with simple protocol combining hemin/G-quadruplex, a mimetic peroxidase, as a catalyzer was proposed and utilized in an electrochemiluminescence biosensor for sensitive microRNA detection.

A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Nanoscale ◽  
2017 ◽  
Vol 9 (42) ◽  
pp. 16149-16153 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hyowon Jang ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Signal Amplification ◽  
Rnase H ◽  
Label Free ◽  
Activity Assay ◽  
G Quadruplex ◽  
Catalytic Hairpin Assembly ◽  
Amplification Strategy

A target-triggered catalytic hairpin assembly with a G-quadruplex specific fluorescent binder, NMM, is employed to develop a novel and sensitive RNase H activity assay.

A QCM Immunosensor for Rapid Determination of Ractopamine in Food Based on Enzyme-AuNPs for Signal Amplification and Magnetic β-Cyclodextrins for Extraction

2015 ◽  
Vol 738-739 ◽  
pp. 56-60
Author(s):  
Shu Xian Chen ◽  
Jing Liu ◽  
Dao Dong Pan ◽  
Ning Gan
Keyword(s):  
Signal Amplification ◽  
Rapid Determination ◽  
Screening Method ◽  
High Sensitivity ◽  
The Novel ◽  
Highly Sensitive ◽  
Reliable Quantification ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

An enzyme amplified immunosensor for highly sensitive detection of Ractopamine (RAC) in foodstuff was developed based on quartz crystal microbalance (QCM). The high sensitivity was achieved by enzyme-AuNPs signal amplification strategy and magnetic β-cyclodextrins (β-CD) enrichment capacity. The novel QCM immunosensor which combines with the advantages of high selectivity of immunoassays and the high sensitivity of QCM has been developed for the determination of trace residues of RAC in food production. Under optimum conditions, the differences in the frequencies (∆f) of the QCM were proportional to the concentration of RAC over the range from 0.01 to 10 ng mL-1. The minimal detection limit was 0.01 ng mL-1. Due to its high sensitivity, acceptable stability and good selectivity, the immunosensor realized reliable quantification of RAC in real foodstuff. The proposed project has the potential to become a successful on-site screening method in food safety.

Ultrasensitive and simple fluorescence biosensor for detection of the mecA gene of Staphylococcus aureus by using an exonuclease III-assisted cascade signal amplification strategy

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5670-5675 ◽  
Author(s):  
Qiong Li ◽  
Danhua Zhou ◽  
Jiafeng Pan ◽  
Zhi Liu ◽  
Junhua Chen
Keyword(s):  
Staphylococcus Aureus ◽  
Signal Amplification ◽  
Meca Gene ◽  
Exonuclease Iii ◽  
G Quadruplex ◽  
Exo Iii ◽  
Fluorescence Biosensor ◽  
Amplification Strategy

An ultrasensitive biosensor was developed for the detection of the mecA gene of Staphylococcus aureus based on the Exo III and G-quadruplex.

An ATP-fueled nucleic acid signal amplification strategy for highly sensitive microRNA detection

2018 ◽  
Vol 54 (77) ◽  
pp. 10897-10900 ◽  
Author(s):  
Zhi-Bin Wen ◽  
Wen-Bin Liang ◽  
Ying Zhuo ◽  
Cheng-Yi Xiong ◽  
Ying-Ning Zheng ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Nucleic Acid ◽  
Signal Amplification ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Strand Displacement ◽  
Microrna Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
Amplification Strategy

Herein, an adenosine triphosphate (ATP)-fueled nucleic acid signal amplification strategy based on toehold-mediated strand displacement (TMSD) and fluorescence resonance energy transfer (FRET) was proposed for highly sensitive detection of microRNA-21.

Sign in / Sign up

Export Citation Format

Share Document