Erythrocytes-based quartz crystal microbalance cytosensor for in situ detection of cell surface sialic acid

The Analyst ◽  
2017 ◽  
Vol 142 (12) ◽  
pp. 2169-2176 ◽  
Author(s):  
Xiaojuan Yang ◽  
Lin Zhou ◽  
Yan Hao ◽  
Bin Zhou ◽  
Peihui Yang
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Quartz Crystal Microbalance ◽  
Signal Amplification ◽  
Quartz Crystal ◽  
In Situ Detection

Erythrocytes-based quartz crystal microbalance cytosensor forin situdetection of cell surface sialic acid using AuNPs/APBA signal amplification nanoprobe.

Ultra-sensitive in situ detection of silver ions using a quartz crystal microbalance

2015 ◽  
Vol 39 (10) ◽  
pp. 8028-8034 ◽  
Author(s):  
Sangmyung Lee ◽  
Kuewhan Jang ◽  
Chanho Park ◽  
Juneseok You ◽  
Taegyu Kim ◽  
...  
Keyword(s):  
Drinking Water ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Silver Ions ◽  
Silver Ion ◽  
Label Free ◽  
Laboratory Conditions ◽  
In Situ Detection

Label-free, ultra-sensitive and in situ detection of silver ion was achieved using a quartz crystal microbalance in laboratory conditions and drinking water.

scholarly journals Exo-enzymatic addition of diazirine-modified sialic acid to cell surfaces enables photocrosslinking of glycoproteins

2021 ◽  
Author(s):  
Nageswari Yarravarapu ◽  
Rohit Sai Reddy Konada ◽  
Narek Darabedian ◽  
Nichole J. Pedowtiz ◽  
Soumya N. Krishnamurthy ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Cell Types ◽  
Cell Surfaces ◽  
Binding Interactions ◽  
Multiple Cell ◽  
Labeling Method ◽  
Cell Surface Glycoconjugates

Glycan binding often mediates extracellular macromolecular recognition events. Accurate characterization of these binding interactions can be difficult because of dissociation and scrambling that occur during purification and analysis steps. Use of photocrosslinking methods has been pursued to covalently capture glycan-dependent interactions in situ however use of metabolic glycan engineering methods to incorporate photocrosslinking sugar analogs is limited to certain cell types. Here we report an exo-enzymatic labeling method to add a diazirine-modified sialic acid (SiaDAz) to cell surface glycoconjugates. The method involves chemoenzymatic synthesis of diazirine-modified CMP-sialic acid (CMP-SiaDAz), followed by sialyltransferase-catalyzed addition of SiaDAz to desialylated cell surfaces. Cell surface SiaDAz-ylation is compatible with multiple cell types and is facilitated by endogenous extracellular sialyltransferase activity present in Daudi B cells. This method for extracellular addition of α2-6-linked SiaDAz enables UV-induced crosslinking of CD22, demonstrating the utility for covalent capture of glycan-mediated binding interactions.

scholarly journals Synthesis of acrylamide-based block-copolymer brushes under flow: monitoring real-time growth and surface restructuring upon drying

2020 ◽  
Vol 11 (18) ◽  
pp. 3209-3216 ◽  
Author(s):  
Joydeb Mandal ◽  
Andrea Arcifa ◽  
Nicholas D. Spencer
Keyword(s):  
Block Copolymer ◽  
Real Time ◽  
Quartz Crystal Microbalance ◽  
Continuous Flow ◽  
Quartz Crystal ◽  
Water Soluble ◽  
Surface Restructuring ◽  
Flow Monitoring ◽  
Copolymer Brushes

Block-copolymer brushes of water-soluble acrylamides have been synthesised by SI-ATRP under continuous flow and their growth monitored in situ by means of a quartz-crystal microbalance with dissipation (QCM-D).

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