Ultra-sensitive in situ detection of silver ions using a quartz crystal microbalance

2015 ◽  
Vol 39 (10) ◽  
pp. 8028-8034 ◽  
Author(s):  
Sangmyung Lee ◽  
Kuewhan Jang ◽  
Chanho Park ◽  
Juneseok You ◽  
Taegyu Kim ◽  
...  
Keyword(s):  
Drinking Water ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Silver Ions ◽  
Silver Ion ◽  
Label Free ◽  
Laboratory Conditions ◽  
In Situ Detection

Label-free, ultra-sensitive and in situ detection of silver ion was achieved using a quartz crystal microbalance in laboratory conditions and drinking water.

Erythrocytes-based quartz crystal microbalance cytosensor for in situ detection of cell surface sialic acid

The Analyst ◽  
2017 ◽  
Vol 142 (12) ◽  
pp. 2169-2176 ◽  
Author(s):  
Xiaojuan Yang ◽  
Lin Zhou ◽  
Yan Hao ◽  
Bin Zhou ◽  
Peihui Yang
Keyword(s):  
Sialic Acid ◽  
Cell Surface ◽  
Quartz Crystal Microbalance ◽  
Signal Amplification ◽  
Quartz Crystal ◽  
In Situ Detection

Erythrocytes-based quartz crystal microbalance cytosensor forin situdetection of cell surface sialic acid using AuNPs/APBA signal amplification nanoprobe.

scholarly journals Quantitative Assessment of Periodontal Bacteria Using a Cell-Based Immunoassay with Functionalized Quartz Crystal Microbalance

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 159
Author(s):  
Satit Rodphukdeekul ◽  
Miyuki Tabata ◽  
Chindanai Ratanaporncharoen ◽  
Yasuo Takeuchi ◽  
Pakpum Somboon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Quantitative Assessment ◽  
Quartz Crystal ◽  
Dynamic Range ◽  
Gold Surface ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Disorder ◽  
Label Free ◽  
Periodontal Bacteria

Periodontal disease is an inflammatory disorder that is triggered by bacterial plaque and causes the destruction of the tooth-supporting tissues leading to tooth loss. Several bacteria species, including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, are considered to be associated with severe periodontal conditions. In this study, we demonstrated a quartz crystal microbalance (QCM) immunoassay for quantitative assessment of the periodontal bacteria, A. actinomycetemcomitans. An immunosensor was constructed using a self-assembled monolayer of 11-mercaptoundecanoic acid (11-MUA) on the gold surface of a QCM chip. The 11-MUA layer was evaluated using a cyclic voltammetry technique to determine its mass and packing density. Next, a monoclonal antibody was covalently linked to 11-MUA using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to act as the biorecognition element. The specificity of the monoclonal antibody was confirmed by an enzyme-linked immunosorbent assay. A calibration curve, for the relationship between the frequency shifts and number of bacteria, was used to calculate the number of A. actinomycetemcomitans bacteria in a test sample. Based on a regression equation, the lower detection limit was 800 cells, with a dynamic range up to 2.32 × 106 cells. Thus, the QCM biosensor in this study provides a sensitive and label-free method for quantitative analysis of periodontal bacteria. The method can be used in various biosensing assays for practical application and routine detection of periodontitis pathogens.

scholarly journals Fate of Salmonella Typhimurium in laboratory-scale drinking water biofilms

2013 ◽  
Vol 11 (4) ◽  
pp. 629-635 ◽  
Author(s):  
L. M. Schaefer ◽  
V. S. Brözel ◽  
S. N. Venter
Keyword(s):  
Drinking Water ◽  
Green Fluorescent Protein ◽  
Health Risk ◽  
Salmonella Typhimurium ◽  
Fluorescent Protein ◽  
Laboratory Scale ◽  
Green Fluorescent ◽  
In Situ Detection

Investigations were carried out to evaluate and quantify colonization of laboratory-scale drinking water biofilms by a chromosomally green fluorescent protein (gfp)-tagged strain of Salmonella Typhimurium. Gfp encodes the green fluorescent protein and thus allows in situ detection of undisturbed cells and is ideally suited for monitoring Salmonella in biofilms. The fate and persistence of non-typhoidal Salmonella in simulated drinking water biofilms was investigated. The ability of Salmonella to form biofilms in monoculture and the fate and persistence of Salmonella in a mixed aquatic biofilm was examined. In monoculture S. Typhimurium formed loosely structured biofilms. Salmonella colonized established multi-species drinking water biofilms within 24 hours, forming micro-colonies within the biofilm. S. Typhimurium was also released at high levels from the drinking water-associated biofilm into the water passing through the system. This indicated that Salmonella could enter into, survive and grow within, and be released from a drinking water biofilm. The ability of Salmonella to survive and persist in a drinking water biofilm, and be released at high levels into the flow for recolonization elsewhere, indicates the potential for a persistent health risk to consumers once a network becomes contaminated with this bacterium.

scholarly journals Synthesis of acrylamide-based block-copolymer brushes under flow: monitoring real-time growth and surface restructuring upon drying

2020 ◽  
Vol 11 (18) ◽  
pp. 3209-3216 ◽  
Author(s):  
Joydeb Mandal ◽  
Andrea Arcifa ◽  
Nicholas D. Spencer
Keyword(s):  
Block Copolymer ◽  
Real Time ◽  
Quartz Crystal Microbalance ◽  
Continuous Flow ◽  
Quartz Crystal ◽  
Water Soluble ◽  
Surface Restructuring ◽  
Flow Monitoring ◽  
Copolymer Brushes

Block-copolymer brushes of water-soluble acrylamides have been synthesised by SI-ATRP under continuous flow and their growth monitored in situ by means of a quartz-crystal microbalance with dissipation (QCM-D).

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