A universal fluorometric assay strategy for glycosidases based on functional carbon quantum dots: β-galactosidase activity detection in vitro and in living cells

2017 ◽  
Vol 5 (10) ◽  
pp. 1971-1979 ◽  
Author(s):  
Cong Tang ◽  
Jin Zhou ◽  
Zhaosheng Qian ◽  
Yanyan Ma ◽  
Yuanyuan Huang ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Carbon Quantum Dots ◽  
Living Cells ◽  
Fluorometric Assay ◽  
Analytical Performance ◽  
Galactosidase Activity ◽  
Activity Detection ◽  
In Cells

A general assay strategy for glycosidase was developed based on functional CQDs, and the assay shows a good analytical performance in vitro and in cells.

Preparation, characterization and in vitro anticancer performance of nanoconjugate based on carbon quantum dots and 5-Fluorouracil

2021 ◽  
Vol 120 ◽  
pp. 111781
Author(s):  
Elaine S.M. Cutrim ◽  
André A.M. Vale ◽  
Danilo Manzani ◽  
Hernane S. Barud ◽  
Enrique Rodríguez-Castellón ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Carbon Quantum Dots

A fluorescent light-up probe based on AIE and ESIPT processes for β-galactosidase activity detection and visualization in living cells

2015 ◽  
Vol 3 (47) ◽  
pp. 9168-9172 ◽  
Author(s):  
Lu Peng ◽  
Meng Gao ◽  
Xiaolei Cai ◽  
Ruoyu Zhang ◽  
Kai Li ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Fluorescent Light ◽  
Galactosidase Activity ◽  
Activity Detection

A fluorescent probe with both AIE and ESIPT characteristics is developed for a β-galactosidase activity study in solution and living cells.

scholarly journals FLASH in Cells

1998 ◽  
Vol 6 (6) ◽  
pp. 3-4
Author(s):  
Stephen W. Carmichael
Keyword(s):  
Traditional Approach ◽  
Living Cells ◽  
Specific Localization ◽  
Fluorescent Molecules ◽  
In Cells

The ability to attach fluorescent molecules onto proteins has allowed specific localization of these proteins within living cells. The traditional approach has been to purify the protein of interest then attach a fluorescent (or another useful tag) molecule in vitro, then somehow introduce the labeled protein into the cell. Whereas this and related techniques have been very useful, there are limitations because each of these three steps is often difficult. Wouldn't it be great if we had a “lock and key” system whereby a protein of interest could be modified to be a specific “lock” and a small tsggable molecule (the “key“) could diffuse into the cell and plug specifically into the lock? Albert Griffin, Stephen Adams, and Roger Tsien have developed such a system

scholarly journals Quantifying Cytosolic Cytochrome c Concentration Using Carbon Quantum Dots as a Powerful Method for Apoptosis Detection

Pharmaceutics ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1556
Author(s):  
Cristian Silviu Moldovan ◽  
Anca Onaciu ◽  
Valentin Toma ◽  
Radu Marginean ◽  
Alin Moldovan ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Cytochrome C ◽  
Laser Scanning ◽  
A549 Cells ◽  
Carbon Quantum Dots ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Fluorescent Nanoparticles ◽  
Cyt C

Background: Cytochrome c (Cyt c) is a key biomarker for early apoptosis, and many methods were designed to detect its release from mitochondria. For a proper evaluation of these programed cell death mechanisms, fluorescent nanoparticles are excellent candidates due to their valuable optical properties. Among all classes of nanoparticles developed thus far, carbon-based quantum dots bring qualitative and efficient imaging strategies for biomedical applications as a consequence of their biocompatibility and low cytotoxicity. Methods: In this study, we synthesized carbon quantum dots smaller than 5 nm from sodium citrate and polyethylene imine. These nanoparticles were rigorously characterized, and their quenching capacity in apoptotic events was assessed in A549 cells treated with staurosporine and etoposide. For the evaluation of Cyt c release, a phenomenon directly correlated with apoptotic events, we ran a semiquantitative analysis using confocal laser scanning microscopy. Results: Carbon quantum dots were synthesized and were successfully employed for Cyt c detection by means of fluorescence microscopy. Significant drops in fluorescence intensity were observed in the case of cells treated with apoptosis-inducing therapeutic compounds compared to untreated cells, confirming Cyt c release from mitochondria to cytosol. Conclusion: Considering these results, we strongly believe this method can contribute to an indirect in vitro evaluation of apoptosis.

A fluorometric assay for acetylcholinesterase activity and inhibitor screening with carbon quantum dots

2016 ◽  
Vol 222 ◽  
pp. 879-886 ◽  
Author(s):  
Zhaosheng Qian ◽  
Lujing Chai ◽  
Cong Tang ◽  
Yuanyuan Huang ◽  
Jianrong Chen ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Acetylcholinesterase Activity ◽  
Carbon Quantum Dots ◽  
Fluorometric Assay ◽  
Inhibitor Screening

Azithromycin detection in cells and tablets by N,S co-doped carbon quantum dots

2021 ◽  
Vol 252 ◽  
pp. 119506
Author(s):  
Xueqing Guo ◽  
Yang Liu ◽  
Wenjuan Dong ◽  
Qin Hu ◽  
Yong Li ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Carbon Quantum Dots ◽  
Co Doped ◽  
In Cells

scholarly journals Kropki kwantowe węgla (CQD) przygotowane z biomasy odpadowej jako nowa klasa biomateriałów o właściwościach luminescencyjnych

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Łukasz JANUS ◽  
Marek PIĄTKOWSKI ◽  
Julia RADWAN-PRAGŁOWSKA ◽  
Aleksandra SIERAKOWSKA
Keyword(s):  
Quantum Dots ◽  
Carbon Quantum Dots

Kropki kwantowe węgla (Carbon Quantum Dots – CQD) to obiekty o rozmiarze mniejszym niż 10 nm, które mają zdolność emitowania promieniowania w zakresie widzialnym od od niebieskiego do czerwonego w zależności od zastosowanego promieniowaniawzbudzenia. Kropki kwantowe stosuje się w bioobrazowaniu in vitro struktur komórkowych lub tworzeniu bioczujników. W przeciwieństwie do klasycznych nanodotów, które są otrzymywane z prostych siarczków, selenków lub tellurków metali, kropki kwantowwęgla są zbudowane z nietoksycznego, biokompatybilnego rdzenia węglowego, dzięki czemu możliwe jest zastosowanie kwantowychkropek węgla w bioobrazowanie struktur biologicznych in vitro lub in vivo przy minimalnym działaniu cytotoksycznym na komórkiCelem badań było uzyskanie nanodotów węglowych zdolnych do emitowania fluorescencji przy użyciu ligniny z biomasy odpadowejCQD sfunkcjonalizowano aminokwasami. Rezultatem prac było uzyskanie serii CQD o zaawansowanych właściwościach luminecencyjnych z zastosowaniem metod hydrotermalnych i mikrofalowych. Gotowe produkty badano pod kątem ich cytotoksyczności

A selective and light-up fluorescent probe for β-galactosidase activity detection and imaging in living cells based on an AIE tetraphenylethylene derivative

2017 ◽  
Vol 53 (32) ◽  
pp. 4505-4508 ◽  
Author(s):  
Guoyu Jiang ◽  
Guanjie Zeng ◽  
Wenping Zhu ◽  
Yongdong Li ◽  
Xiaobiao Dong ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Detection ◽  
Living Cells ◽  
Galactosidase Activity ◽  
Activity Detection

An AIE based tetraphenylethylene derivative (TPE-Gal) was designed for light-up fluorescence detection and imaging of β-galactosidase activity in living cells.

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