Membranes under shear stress: visualization of non-equilibrium domain patterns and domain fusion in a microfluidic device

Soft Matter ◽  
2016 ◽  
Vol 12 (23) ◽  
pp. 5072-5076 ◽  
Author(s):  
Flurin Sturzenegger ◽  
Tom Robinson ◽  
David Hess ◽  
Petra S. Dittrich
Keyword(s):  
Shear Stress ◽  
Microfluidic Device ◽  
Domain Fusion ◽  
Non Equilibrium

scholarly journals Quantification of fast molecular adhesion by fluorescence footprinting

2021 ◽  
Author(s):  
Adam B. Yasunaga ◽  
Isaac T.S. Li
Keyword(s):  
Shear Stress ◽  
Dynamic Range ◽  
Force Distribution ◽  
Equilibrium Conditions ◽  
Ligand Dissociation ◽  
Molecular Adhesion ◽  
Molecular Force ◽  
Adhesion Complex ◽  
Non Equilibrium

AbstractRolling adhesion is a unique process in which the adhesion events are short-lived and operate under highly non-equilibrium conditions. These characteristics pose a challenge in molecular force quantification, where in situ measurement of such forces cannot be achieved with most molecular force sensors that probe near equilibrium. In this report, we demonstrated a quantitative adhesion footprint assay combining DNA-based non-equilibrium force probes and modelling to measure the molecular force involved in fast rolling adhesion. We were able to directly profile the ensemble molecular force distribution during rolling adhesion with a dynamic range between 0 – 18 pN. Our results showed that the shear stress driving bead rolling motility directly controls the molecular tension on the probe-conjugated adhesion complex. Furthermore, the shear stress can steer the dissociation bias of components within the molecular force probe complex, favouring either DNA probe dissociation or receptor-ligand dissociation.

scholarly journals A tapered channel microfluidic device for comprehensive cell adhesion analysis, using measurements of detachment kinetics and shear stress-dependent motion

2012 ◽  
Vol 6 (1) ◽  
pp. 014107 ◽  
Author(s):  
Peter Rupprecht ◽  
Laurent Golé ◽  
Jean-Paul Rieu ◽  
Cyrille Vézy ◽  
Rosaria Ferrigno ◽  
...  
Keyword(s):  
Shear Stress ◽  
Cell Adhesion ◽  
Microfluidic Device ◽  
Tapered Channel ◽  
Stress Dependent

scholarly journals Endothelial cell polarization and orientation to flow in a novel microfluidic multimodal shear stress generator

Lab on a Chip ◽  
2020 ◽  
Vol 20 (23) ◽  
pp. 4373-4390
Author(s):  
Utku M. Sonmez ◽  
Ya-Wen Cheng ◽  
Simon C. Watkins ◽  
Beth L. Roman ◽  
Lance A. Davidson
Keyword(s):  
Shear Stress ◽  
Endothelial Cell ◽  
Microfluidic Device ◽  
Cell Polarization ◽  
Orientation Analysis ◽  
Stress Gradients ◽  
Multiple Levels

Endothelial cell polarization and orientation analysis using a novel microfluidic device that can simultaneously generate multiple levels of shear stress and shear stress gradients for systematic mechanobiology studies under flow.

scholarly journals Manipulating shear-induced non-equilibrium transitions in colloidal films by feedback control

Soft Matter ◽  
2015 ◽  
Vol 11 (2) ◽  
pp. 406-413 ◽  
Author(s):  
Tarlan A. Vezirov ◽  
Sascha Gerloff ◽  
Sabine H. L. Klapp
Keyword(s):  
Shear Stress ◽  
Feedback Control ◽  
Brownian Dynamics ◽  
Colloidal Films ◽  
Non Equilibrium

Using Brownian Dynamics (BD) simulations we investigate non-equilibrium transitions of sheared colloidal films under controlled shear stress σxz.

Study of Long Term Viability of Endothelial Cells on Biochip for Rapid and Reliable Water Toxicity Measurements

2013 ◽  
Author(s):  
Fei Liu ◽  
Tingting Chen ◽  
Xudong Zhang ◽  
Fang Li ◽  
Ioana Voiculescu
Keyword(s):  
Shear Stress ◽  
Microfluidic Device ◽  
Cell Attachment ◽  
Good Health ◽  
Low Flow ◽  
Live Cells ◽  
Toxicity Tests ◽  
Bovine Aortic Endothelial Cell ◽  
Microfluidic Channels ◽  
Water Toxicity

Measuring water toxicity is a lengthy process, and rapid analytical methods are limited. A complementary approach is to measure water toxicity on live cells via electric cell-substrate impedance sensing (ECIS) using a field portable device. This paper presents a study of the longevity of bovine aortic endothelial cell (BAECs VEC Technologies, Rensselaer, NY) by integrating a microfluidic device onto the ECIS sensors. This microfluidic chamber with a network of tree-like perfusion microfluidic channels for cell media delivery to the culturing chamber was fabricated from a biocompatible polymer and tested for longevity studies. This perfusion microchannels were designed as a symmetric arbor with binary splitting to provide equal flow in all the perfusion channels. The microdimensions of the perfusion channels provide high flow resistance, thus carrying low flow rates for a given head pressure and generating low shear stress to the cells during the long-time cell attachment and proliferation period. With such a microfluidic device, cell media can be automatically and evenly perfused into the culturing chamber and no significant shear stress produced by media perfusion was observed. During the longevity study, the BAECs were able to survive in good health for longer than one month. Toxicity tests to study the BAECs responsiveness to health-threatening concentrations of ammonia using the microfluidic ECIS sensor will be also presented. Using impedance spectroscopy technique we demonstrated the BAECs can rapidly respond to ammonia concentrations between the military exposure guideline of 2mM and human lethal concentration of 55mM. The BAECs monolayer represent the most important component of a biosensor for testing water toxicity in the field. This research concluded that the BAECs could resist at least 34 days on the microfluidic chip and demonstrate high values of cell membrane impedance during long period of time.

scholarly journals A microfluidic device towards shear stress analysis of clonal expanded endothelial cells

2014 ◽  
Vol 9 (1) ◽  
pp. JBSE0006-JBSE0006
Author(s):  
Emilie WEIBULL ◽  
Shunsuke MATSUI ◽  
Helene ANDERSSON SVAHN ◽  
Toshiro OHASHI
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Stress Analysis ◽  
Microfluidic Device
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