scholarly journals Simultaneous fluorescence imaging of hydrogen peroxide in mitochondria and endoplasmic reticulum during apoptosis

2016 ◽  
Vol 7 (9) ◽  
pp. 6153-6159 ◽  
Author(s):  
Haibin Xiao ◽  
Ping Li ◽  
Xiufen Hu ◽  
Xiaohui Shi ◽  
Wen Zhang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Endoplasmic Reticulum ◽  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Simultaneous Imaging

We have developed two new organelle-specific fluorescent probes for the simultaneous imaging of hydrogen peroxide in the mitochondria and the endoplasmic reticulum during apoptosis.

Simultaneous fluorescence visualization of mitochondrial hydrogen peroxide and zinc ions in live cells and in vivo

2016 ◽  
Vol 52 (86) ◽  
pp. 12741-12744 ◽  
Author(s):  
Haibin Xiao ◽  
Ping Li ◽  
Shan Zhang ◽  
Wei Zhang ◽  
Wen Zhang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probes ◽  
Live Cells ◽  
Zinc Ions ◽  
Simultaneous Imaging

We have developed two new fluorescent probes termedM-H2O2andM-Znfor simultaneous imaging of hydrogen peroxide and zinc ions in mitochondria.

scholarly journals Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes

2013 ◽  
Vol 18 (10) ◽  
pp. 106002 ◽  
Author(s):  
Hengchang Guo ◽  
Hossein Aleyasin ◽  
Scott S. Howard ◽  
Bryan C. Dickinson ◽  
Vivian S. Lin ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Intracellular Hydrogen Peroxide

scholarly journals Intracellular Sources of ROS/H2O2 in Health and Neurodegeneration: Spotlight on Endoplasmic Reticulum

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 233
Author(s):  
Tasuku Konno ◽  
Eduardo Pinho Melo ◽  
Joseph E. Chambers ◽  
Edward Avezov
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Endoplasmic Reticulum ◽  
Neurodegenerative Diseases ◽  
Antioxidative Enzymes ◽  
Redox Reactions ◽  
Ros Production ◽  
Oxygen Species ◽  
Specific Target

Reactive oxygen species (ROS) are produced continuously throughout the cell as products of various redox reactions. Yet these products function as important signal messengers, acting through oxidation of specific target factors. Whilst excess ROS production has the potential to induce oxidative stress, physiological roles of ROS are supported by a spatiotemporal equilibrium between ROS producers and scavengers such as antioxidative enzymes. In the endoplasmic reticulum (ER), hydrogen peroxide (H2O2), a non-radical ROS, is produced through the process of oxidative folding. Utilisation and dysregulation of H2O2, in particular that generated in the ER, affects not only cellular homeostasis but also the longevity of organisms. ROS dysregulation has been implicated in various pathologies including dementia and other neurodegenerative diseases, sanctioning a field of research that strives to better understand cell-intrinsic ROS production. Here we review the organelle-specific ROS-generating and consuming pathways, providing evidence that the ER is a major contributing source of potentially pathologic ROS.

scholarly journals Composition of the redox environment of the endoplasmic reticulum and sources of hydrogen peroxide

2015 ◽  
Vol 83 ◽  
pp. 331-340 ◽  
Author(s):  
Éva Margittai ◽  
Balázs Enyedi ◽  
Miklós Csala ◽  
Miklós Geiszt ◽  
Gábor Bánhegyi
Keyword(s):  
Hydrogen Peroxide ◽  
Endoplasmic Reticulum ◽  
Redox Environment

Fluorescent Probes for Hydrogen Peroxide Based on a Non-Oxidative Mechanism

2004 ◽  
Vol 43 (18) ◽  
pp. 2389-2391 ◽  
Author(s):  
Hatsuo Maeda ◽  
Yuka Fukuyasu ◽  
Shoko Yoshida ◽  
Masako Fukuda ◽  
Kanako Saeki ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probes

PERK mediates oxidative stress and adipogenesis in Graves’ orbitopathy pathogenesis

2021 ◽  
Author(s):  
JaeSang Ko ◽  
Ji-Young Kim ◽  
Min Kyung Chae ◽  
Eun Jig Lee ◽  
Jin Sook Yoon
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Endoplasmic Reticulum ◽  
Transcription Factor ◽  
Er Stress ◽  
Adipogenic Differentiation ◽  
Ros Generation ◽  
Mrna Levels ◽  
Graves Orbitopathy ◽  
Orbital Fibroblasts

We examined endoplasmic reticulum (ER) stress-related gene expression in orbital tissues from patients with Graves’ orbitopathy (GO) and the effects of silencing protein kinase RNA-like endoplasmic reticulum kinase (PERK) in primary orbital fibroblast cultures to demonstrate the therapeutic potential of PERK-modulating agents in GO management. The expression of ER stress related genes in orbital tissue harvested from individuals with or without GO was studied using real-time polymerase chain reaction. The role of PERK in GO pathogenesis was examined through small-interfering RNA (siRNA)-mediated silencing in cultured primary orbital fibroblasts. Intracellular reactive oxygen species (ROS) levels induced in response to cigarette smoke extract (CSE) or hydrogen peroxide were measured using 5-(and 6)-carboxy-20,70-dichlorodihydrofluorescein diacetate staining and flow cytometry. Cells were stained with Oil Red O, and adipogenesis-related transcription factor expression was evaluated through western blotting after adipogenic differentiation. PERK, activating transcription factor 4 (ATF4), and CCAAT-enhancer-binding protein (C/EBP)-homologous protein(CHOP)mRNA levels were significantly higher in GO orbital tissues than in non-GO orbital tissues. PERK silencing inhibited CSE- or hydrogen peroxide-induced ROS generation. After adipogenic differentiation, GO orbital fibroblasts revealed decreased lipid droplets and downregulation of C/EBPα, C/EBPβ, and peroxisome proliferator-activator gamma (PPARγ) in PERK siRNA-transfected cells. The orbital tissues of patients with GO were exposed to chronic ER stress and subsequently exhibited enhanced unfolded protein response (especially through the PERK pathway). PERK silencing reduced oxidative stress and adipogenesis in GO orbital fibroblasts in vitro. Our results imply that PERK-modulating agents can potentially be used to manage GO.

Fluorescent Probes Used for Detection of Hydrogen Peroxide under Biological Conditions

2015 ◽  
Vol 46 (3) ◽  
pp. 171-200 ◽  
Author(s):  
Krzysztof Żamojć ◽  
Magdalena Zdrowowicz ◽  
Dagmara Jacewicz ◽  
Dariusz Wyrzykowski ◽  
Lech Chmurzyński
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probes

scholarly journals Double-stranded DNA-scaffolded fluorescent probes for fluorescence imaging of cell-surface molecules

RSC Advances ◽  
2017 ◽  
Vol 7 (83) ◽  
pp. 52581-52587
Author(s):  
Zhanghua Liu ◽  
Yang Liu ◽  
Yanan Sun ◽  
Guo Chen ◽  
Yong Chen
Keyword(s):  
Cell Surface ◽  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Cell Surface Molecules ◽  
Cell Surfaces ◽  
Surface Molecules ◽  
Double Stranded Dna

Double-stranded DNA-scaffolded fluorescent probes were developed for fluorescence imaging of molecules on cell surfaces.

Why fluorescent probes for endoplasmic reticulum are selective: an experimental and QSAR-modelling study

2003 ◽  
Vol 78 (6) ◽  
pp. 323-332 ◽  
Author(s):  
J Colston ◽  
Rw Horobin ◽  
F Rashid-Doubell ◽  
J Pediani ◽  
Kk Johal
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probes ◽  
Qsar Modelling ◽  
Modelling Study
Sign in / Sign up

Export Citation Format

Share Document