Simultaneous fluorescence visualization of mitochondrial hydrogen peroxide and zinc ions in live cells and in vivo

2016 ◽  
Vol 52 (86) ◽  
pp. 12741-12744 ◽  
Author(s):  
Haibin Xiao ◽  
Ping Li ◽  
Shan Zhang ◽  
Wei Zhang ◽  
Wen Zhang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probes ◽  
Live Cells ◽  
Zinc Ions ◽  
Simultaneous Imaging

We have developed two new fluorescent probes termedM-H2O2andM-Znfor simultaneous imaging of hydrogen peroxide and zinc ions in mitochondria.

Dual-color imaging of cytosolic and mitochondrial zinc ions in live tissues with two-photon fluorescent probes

2014 ◽  
Vol 12 (21) ◽  
pp. 3406-3412 ◽  
Author(s):  
Kailash Rathore ◽  
Chang Su Lim ◽  
Young Lee ◽  
Bong Rae Cho
Keyword(s):  
Fluorescent Probes ◽  
Live Cells ◽  
Zinc Ions ◽  
Color Imaging ◽  
Two Photon ◽  
Dual Color ◽  
Living Tissues

We have developed TP probes for [Zn2+]cyto and [Zn2+]mito, which emit TPEF at widely-separated wavelength regions. The new probes can simultaneously detect [Zn2+]cyto and [Zn2+]mito in live cells, as well as in living tissues by dual-color TPM imaging.

Pyrene based fluorescent probes for detecting endogenous zinc ions in live cells

2015 ◽  
Vol 113 ◽  
pp. 372-377 ◽  
Author(s):  
Ying Hu ◽  
Yifan Liu ◽  
Gyoungmi Kim ◽  
Eun Jin Jun ◽  
K.M.K. Swamy ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Live Cells ◽  
Zinc Ions

scholarly journals Simultaneous fluorescence imaging of hydrogen peroxide in mitochondria and endoplasmic reticulum during apoptosis

2016 ◽  
Vol 7 (9) ◽  
pp. 6153-6159 ◽  
Author(s):  
Haibin Xiao ◽  
Ping Li ◽  
Xiufen Hu ◽  
Xiaohui Shi ◽  
Wen Zhang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Endoplasmic Reticulum ◽  
Fluorescence Imaging ◽  
Fluorescent Probes ◽  
Simultaneous Imaging

We have developed two new organelle-specific fluorescent probes for the simultaneous imaging of hydrogen peroxide in the mitochondria and the endoplasmic reticulum during apoptosis.

scholarly journals Fluorescent probes for in vitro and in vivo quantification of hydrogen peroxide

2020 ◽  
Vol 11 (44) ◽  
pp. 11989-11997
Author(s):  
Sen Ye ◽  
Jun Jacob Hu ◽  
Qian Angela Zhao ◽  
Dan Yang
Keyword(s):  
Hydrogen Peroxide ◽  
Flow Cytometry ◽  
Fluorescent Probes ◽  
Quantitative Measurement ◽  
Disease Models ◽  
New Class ◽  
Imaging Flow Cytometry ◽  
Bio Imaging

New class of H2O2 probes, HKPerox-Red and HKPerox-Ratio, were developed for quantitative measurement of H2O2 generated in multiple disease models using bio-imaging, flow cytometry, and in vitro assays in an ultra-sensitive and selective manner.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

scholarly journals A paradigm shift in cell-free approach: the emerging role of MSCs-derived exosomes in regenerative medicine

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Soudeh Moghadasi ◽  
Marischa Elveny ◽  
Heshu Sulaiman Rahman ◽  
Wanich Suksatan ◽  
Abduladheem Turki Jalil ◽  
...  
Keyword(s):  
Regenerative Medicine ◽  
Ethical Issues ◽  
Therapeutic Potential ◽  
Kidney Diseases ◽  
Experimental Models ◽  
Live Cells ◽  
Target Cells ◽  
Intercellular Interaction ◽  
Micro Rnas

AbstractRecently, mesenchymal stem/stromal cells (MSCs) due to their pro-angiogenic, anti-apoptotic, and immunoregulatory competencies along with fewer ethical issues are presented as a rational strategy for regenerative medicine. Current reports have signified that the pleiotropic effects of MSCs are not related to their differentiation potentials, but rather are exerted through the release of soluble paracrine molecules. Being nano-sized, non-toxic, biocompatible, barely immunogenic, and owning targeting capability and organotropism, exosomes are considered nanocarriers for their possible use in diagnosis and therapy. Exosomes convey functional molecules such as long non-coding RNAs (lncRNAs) and micro-RNAs (miRNAs), proteins (e.g., chemokine and cytokine), and lipids from MSCs to the target cells. They participate in intercellular interaction procedures and enable the repair of damaged or diseased tissues and organs. Findings have evidenced that exosomes alone are liable for the beneficial influences of MSCs in a myriad of experimental models, suggesting that MSC- exosomes can be utilized to establish a novel cell-free therapeutic strategy for the treatment of varied human disorders, encompassing myocardial infarction (MI), CNS-related disorders, musculoskeletal disorders (e.g. arthritis), kidney diseases, liver diseases, lung diseases, as well as cutaneous wounds. Importantly, compared with MSCs, MSC- exosomes serve more steady entities and reduced safety risks concerning the injection of live cells, such as microvasculature occlusion risk. In the current review, we will discuss the therapeutic potential of MSC- exosomes as an innovative approach in the context of regenerative medicine and highlight the recent knowledge on MSC- exosomes in translational medicine, focusing on in vivo researches.

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