scholarly journals Ultrahigh protein adsorption capacity and sustained release of nanocomposite scaffolds: implication for growth factor delivery systems

RSC Advances ◽  
2017 ◽  
Vol 7 (27) ◽  
pp. 16453-16459 ◽  
Author(s):  
Ji-Young Yoon ◽  
Jung-Ju Kim ◽  
Ahmed El-Fiqi ◽  
Jun-Hyeog Jang ◽  
Hae-Won Kim
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Sustained Release ◽  
Protein Adsorption ◽  
Adsorption Capacity ◽  
Delivery Systems ◽  
Growth Factor Delivery ◽  
Load Growth ◽  
Nanocomposite Scaffolds

Nanocomposite scaffolds that can load growth factors effectively and release them sustainably are developed for the regeneration of tissues.

scholarly journals Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-Like Phenotype in Adipose-Derived Stem Cells

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3123
Author(s):  
Brandan Walters ◽  
Paul A. Turner ◽  
Bernd Rolauffs ◽  
Melanie L. Hart ◽  
Jan P. Stegemann
Keyword(s):  
Stem Cells ◽  
Smooth Muscle ◽  
Growth Factors ◽  
Growth Factor ◽  
Sustained Release ◽  
Smooth Muscle Cell ◽  
Cell Morphology ◽  
Cyclic Stretch ◽  
Adipose Derived Stem Cells ◽  
Growth Factor Delivery

Adipose-derived stem cells (ASCs) are an abundant and easily accessible multipotent stem cell source with potential application in smooth muscle regeneration strategies. In 3D collagen hydrogels, we investigated whether sustained release of growth factors (GF) PDGF-AB and TGF-β1 from GF-loaded microspheres could induce a smooth muscle cell (SMC) phenotype in ASCs, and if the addition of uniaxial cyclic stretch could enhance the differentiation level. This study demonstrated that the combination of cyclic stretch and GF release over time from loaded microspheres potentiated the differentiation of ASCs, as quantified by protein expression of early to late SMC differentiation markers (SMA, TGLN and smooth muscle MHC). The delivery of GFs via microspheres produced large ASCs with a spindle-shaped, elongated SMC-like morphology. Cyclic strain produced the largest, longest, and most spindle-shaped cells regardless of the presence or absence of growth factors or the growth factor delivery method. Protein expression and cell morphology data confirmed that the sustained release of GFs from GF-loaded microspheres can be used to promote the differentiation of ASCs into SMCs and that the addition of uniaxial cyclic stretch significantly enhances the differentiation level, as quantified by intermediate and late SMC markers and a SMC-like elongated cell morphology.

scholarly journals Growth Factor Therapy for Parkinson’s Disease: Alternative Delivery Systems

2021 ◽  
pp. 1-7
Author(s):  
Sarah Jarrin ◽  
Abrar Hakami ◽  
Ben Newland ◽  
Eilís Dowd
Keyword(s):  
Parkinson’S Disease ◽  
Gene Therapy ◽  
Parkinson's Disease ◽  
Growth Factors ◽  
Growth Factor ◽  
Ex Vivo ◽  
Brain Regions ◽  
Delivery Systems ◽  
Alternative Delivery

Despite decades of research and billions in global investment, there remains no preventative or curative treatment for any neurodegenerative condition, including Parkinson’s disease (PD). Arguably, the most promising approach for neuroprotection and neurorestoration in PD is using growth factors which can promote the growth and survival of degenerating neurons. However, although neurotrophin therapy may seem like the ideal approach for neurodegenerative disease, the use of growth factors as drugs presents major challenges because of their protein structure which creates serious hurdles related to accessing the brain and specific targeting of affected brain regions. To address these challenges, several different delivery systems have been developed, and two major approaches—direct infusion of the growth factor protein into the target brain region and in vivo gene therapy—have progressed to clinical trials in patients with PD. In addition to these clinically evaluated approaches, a range of other delivery methods are in various degrees of development, each with their own unique potential. This review will give a short overview of some of these alternative delivery systems, with a focus on ex vivo gene therapy and biomaterial-aided protein and gene delivery, and will provide some perspectives on their potential for clinical development and translation.

Collagen fibrous scaffolds for sustained delivery of growth factors for meniscal tissue engineering

Nanomedicine ◽  
2022 ◽  
Author(s):  
Jihye Baek ◽  
Kwang Il Lee ◽  
Ho Jong Ra ◽  
Martin K Lotz ◽  
Darryl D D'Lima
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Sustained Release ◽  
Ex Vivo ◽  
Synovial Cell ◽  
Growth Factor Delivery ◽  
Meniscal Tissue ◽  
A Cell

Aim: To mimic the ultrastructural morphology of the meniscus with nanofiber scaffolds coupled with controlled growth factor delivery to modulate cellular performance for tissue engineering of menisci. Methods: The authors functionalized collagen nanofibers by conjugating heparin to the following growth factors for sustained release: PDGF-BB, TGF-β1 and CTGF. Results: Incorporating growth factors increased human meniscal and synovial cell viability, proliferation and infiltration in vitro, ex vivo and in vivo; upregulated key genes involved in meniscal extracellular matrix synthesis; and enhanced generation of meniscus-like tissue. Conclusion: The authors' results indicate that functionalizing collagen nanofibers can create a cell-favorable micro- and nanoenvironment and can serve as a system for sustained release of bioactive factors.

Development of growth factor-incorporating liposomes for integration into scaffolds as a method to improve tissue regeneration

2021 ◽  
Author(s):  
E. Natsaridis ◽  
P. Mouzoura ◽  
F. Gkartziou ◽  
A. Marazioti ◽  
S.G. Antimisiaris
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Tissue Regeneration ◽  
Structural Characteristics ◽  
Drug Carriers ◽  
Growth Factor Delivery ◽  
Liposomal Form ◽  
Liposomal Drug ◽  
Hydrophilic Nature ◽  
Rapid Clearance

This review is an update about the efforts to develop liposomal carriers for growth factor delivery. It is well known that growth factors have the potential to enhance/accelerate tissue regeneration, however their poor stability which results in rapid loss of their activity, together with their rapid clearance from defected tissues (when applied as free molecules) is a serious drawback for their use; their highly hydrophilic nature and low capability to permeate through biological barriers (cell membranes) are additional factors that limit their applicability. In the last years, the advantages of liposomal drug delivery systems have motivated efforts to deliver growth factors (GFs) in liposomal form. Herein, after briefly introducing the basic structural characteristics of liposome types and their advantages when used as drug carriers, as well as the basic problems encountered when GFs are applied for tissue regeneration, we focus on recent reports about development and potential regenerative effects of liposomal GFs, towards defects of various tissues. The methodologies used for incorporation, attachment or immobilization of liposomal GFs in order to sustain their retention at the defected tissues, are highlighted as well.

scholarly journals PEG grafted chitosan scaffold for dual growth factor delivery for enhanced wound healing

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Amritha Vijayan ◽  
Sabareeswaran A. ◽  
G. S. Vinod Kumar
Keyword(s):  
Wound Healing ◽  
Growth Factors ◽  
Growth Factor ◽  
Treated Group ◽  
Growth Factor Delivery ◽  
Chitosan Scaffold ◽  
Collagen Formation ◽  
Dual Growth Factor Delivery

AbstractApplication of growth factors at wound site has improved the efficiency and quality of healing. Basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) induce proliferation of various cells in wound healing. Delivery of growth factor from controlled release systems protect it from degradation and also result in sustained delivery of it at the site of injury. The goal of the study was to develop a Polyethylene glycol (PEG) cross-linked cotton-like chitosan scaffold (CS-PEG-H) by freeze-drying method and chemically conjugate heparin to the scaffold to which the growth factors can be electrostatically bound and evaluate its wound healing properties in vitro and in vivo. The growth factor containing scaffolds induced increased proliferation of HaCaT cells, increased neovascularization and collagen formation seen by H and E and Masson’s trichrome staining. Immunohistochemistry was performed using the Ki67 marker which increased proliferation of cells in growth factor containing scaffold treated group. Frequent dressing changes are a major deterrent to proper wound healing. Our system was found to release both VEGF and bFGF in a continuous manner and attained stability after 7 days. Thus our system can maintain therapeutic levels of growth factor at the wound bed thereby avoiding the need for daily applications and frequent dressing changes. Thus, it can be a promising candidate for wound healing.

Release of Growth Factors from Gelatin Microsphere/CaP Composites

2007 ◽  
Vol 361-363 ◽  
pp. 527-530 ◽  
Author(s):  
W.J.E.M. Habraken ◽  
O.C. Boerman ◽  
Joop G.C. Wolke ◽  
Antonious G. Mikos ◽  
John A. Jansen
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Sustained Release ◽  
In Vitro Release ◽  
Gelatin Microspheres ◽  
Or Efficiency ◽  
Loading Method ◽  
Gelatin Microsphere ◽  
Release Curve

Composites of gelatin microspheres and injectable calcium phosphate cement were prepared to increase cement resorption and improve tissue ingrowth. To further enhance these properties, osteoinductive growth factors can be introduced into the microspheres. In this study, the in vitro release of preset gelatin microsphere/CaP composites was followed for 6 weeks by use of 125I-labelled rhBMP-2, rhTGF-β and rh-bFGF. Results for all gelatin microsphere composites showed a release curve that consisted of a small burst, followed by a sustained release. The magnitude of the sustained release was dependent on the growth factor used, and showed a slight dependency on the loading method and type of gelatin. Furthermore, no differences in release pattern or efficiency were found when growth factor concentration increased.

scholarly journals Integrated approach to designing growth factor delivery systems

2007 ◽  
Vol 21 (14) ◽  
pp. 3896-3903 ◽  
Author(s):  
Ruth R. Chen ◽  
Eduardo A. Silva ◽  
William W. Yuen ◽  
Andrea A. Brock ◽  
Claudia Fischbach ◽  
...  
Keyword(s):  
Growth Factor ◽  
Integrated Approach ◽  
Delivery Systems ◽  
Growth Factor Delivery

scholarly journals Biomimetic proteoglycan nanoparticles for growth factor immobilization and delivery

2020 ◽  
Vol 8 (4) ◽  
pp. 1127-1136 ◽  
Author(s):  
Nooshin Zandi ◽  
Ebrahim Mostafavi ◽  
Mohammad Ali Shokrgozar ◽  
Elnaz Tamjid ◽  
Thomas J. Webster ◽  
...  
Keyword(s):  
Growth Factors ◽  
Controlled Release ◽  
Growth Factor ◽  
Half Life ◽  
Growth Factor Delivery ◽  
Short Half Life

Growth factor delivery is often challenging due to their short half-life, low stability, and rapid deactivation. Here, we engineered novel biomimetic proteoglycan nanocarriers for the immobilization and controlled release of growth factors.

Extracellular matrix-inspired growth factor delivery systems for bone regeneration

2015 ◽  
Vol 94 ◽  
pp. 41-52 ◽  
Author(s):  
Mikaël M. Martino ◽  
Priscilla S. Briquez ◽  
Kenta Maruyama ◽  
Jeffrey A. Hubbell
Keyword(s):  
Extracellular Matrix ◽  
Growth Factor ◽  
Bone Regeneration ◽  
Delivery Systems ◽  
Growth Factor Delivery

scholarly journals Extracellular Matrix-Inspired Growth Factor Delivery Systems for Skin Wound Healing

2015 ◽  
Vol 4 (8) ◽  
pp. 479-489 ◽  
Author(s):  
Priscilla S. Briquez ◽  
Jeffrey A. Hubbell ◽  
Mikaël M. Martino
Keyword(s):  
Extracellular Matrix ◽  
Wound Healing ◽  
Growth Factor ◽  
Delivery Systems ◽  
Skin Wound ◽  
Growth Factor Delivery ◽  
Skin Wound Healing
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