scholarly journals Development and multi-use applications of dengue NS1 monoclonal antibody for early diagnosis

RSC Advances ◽  
2017 ◽  
Vol 7 (4) ◽  
pp. 1927-1931 ◽  
Author(s):  
E. Kathiresan ◽  
R. Paramasivan ◽  
V. Thenmozhi ◽  
Aparup Das ◽  
K. J. Dhananjeyan ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Early Diagnosis ◽  
Case Management ◽  
Epidemiological Purpose

Swift and early diagnosis of dengue is important for case management and epidemiological purpose.

scholarly journals Anti-IgE Monoclonal Antibody in Allergic Bronchopulmonary Aspergillosis Not Responding to Systemic Steroids

2021 ◽  
Author(s):  
Pratibha Sharma ◽  
Ranganath T. Ganga
Keyword(s):  
Monoclonal Antibody ◽  
Early Diagnosis ◽  
Aspergillus Fumigatus ◽  
Standard Treatment ◽  
Treatment Options ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
The Other ◽  
Successful Management ◽  
Systemic Steroids ◽  
Pulse Steroids

AbstractAllergic bronchopulmonary aspergillosis (ABPA) is a hypersensitivity disorder caused by Aspergillus fumigatus commonly among patients of bronchial asthma. Early diagnosis is the key to successful management and preventing parenchymal destruction. Most of the patients respond well to systemic steroids. In patients not responding to standard treatment, the other treatment options include pulse steroids, antifungals, immunosuppressants, and omalizumab. But exact indication for each of these is not well established. Here we report a 41-year-old ABPA patient who was not responding to systemic steroids became totally asymptomatic with the administration of omalizumab.

646 Early Diagnosis of Precancerous Stage of Ductal Adenocarcinoma of the Pancreas Using a Novel Monoclonal Antibody

2008 ◽  
Vol 134 (4) ◽  
pp. A-93
Author(s):  
Daphne C. Ang ◽  
Angela Sefah ◽  
Kelly A. Walton ◽  
Malca Kierson ◽  
Michael S. Nagar ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Early Diagnosis ◽  
Ductal Adenocarcinoma ◽  
Precancerous Stage ◽  
Adenocarcinoma Of The Pancreas

scholarly journals Conventional diagnostic aids in dental caries

2019 ◽  
Vol 2 ◽  
pp. 53-58
Author(s):  
Adepu Srilatha ◽  
Dolar Doshi ◽  
Suhas Kulkarni ◽  
Madupu Reddy
Keyword(s):  
Dental Caries ◽  
Early Diagnosis ◽  
Oral Cavity ◽  
Case Management ◽  
Sensitivity And Specificity ◽  
Diagnostic Tool ◽  
Cognitive Skills ◽  
Visual Assessment ◽  
Diagnostic Modalities

Dentistry is rapidly evolving from a surgical and reparative profession into a healing profession focused on overall patient wellness. The oral systematic connection has been well established, with the condition and status of the oral cavity being a great indicator of the patient’s overall health. In today’s scenario, most of the focus is towards prevention, early diagnosis, and intervention to minimize treatment, to enable the most desirable outcomes. Thus, the dental practitioner’s should have efficient cognitive skills to be a diagnostician and for case management. Furthermore, the diagnostic modalities available have been expanded greatly on the foundation of a comprehensive visual assessment, with a ultimate goal of improving both the sensitivity and specificity level for any caries detecting diagnostic tool.

Monoclonal antibody inhibiting creatine kinase MM3 but not isoform MM1

1990 ◽  
Vol 36 (1) ◽  
pp. 153-156 ◽  
Author(s):  
T Suzuki ◽  
T Shiraishi ◽  
K Tomita ◽  
M Totani ◽  
T Murachi
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Monoclonal Antibody ◽  
Creatine Kinase ◽  
Early Diagnosis ◽  
Human Serum ◽  
Carboxypeptidase N ◽  
Analytical Separation ◽  
Diagnostic Reagent ◽  
Inhibition Index

Abstract Monoclonal antibody CKM-G01 inhibited greater than 99% of the activity of porcine and human creatine kinase(CK)-MM isoenzyme purified from muscle. However, it inhibited only 54% of CK-MM in human serum. Chromatofocusing of serum CK-MM showed that CKM-G01 inhibited 100% of MM3 but not isoform MM1. CKM-G01 inhibited CK-MM2 by 57%. CKM-G01 specifically inhibited only the original CK-M subunit and not the subunit modified by removal of C-terminal lysine by carboxypeptidase N. CKM-G01 can be used for assay of CK isoforms. We devised a new diagnostic reagent involving it, which requires no analytical separation of isoforms, based on the immunoinhibition method, and applied it to early diagnosis of acute myocardial infarction. The "inhibition index," (inhibited CK activity/total CK activity) x 100, increased more rapidly than did total CK and CK-MB. Evidently this diagnostic reagent can be used for easy, early diagnosis of acute myocardial infarction.

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

High-voltage electron microscopy of subretinal scar formation

1992 ◽  
Vol 50 (1) ◽  
pp. 486-487
Author(s):  
G.E. Korte ◽  
M. Marko ◽  
G. Hageman
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Retinal Pigment Epithelium ◽  
Glial Cells ◽  
High Voltage ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Sodium Iodate ◽  
High Voltage Electron Microscopy ◽  
Voltage Electron

Sodium iodate iv. damages the retinal pigment epithelium (RPE) in rabbits. Where RPE does not regenerate (e.g., 1,2) Muller glial cells (MC) forma subretinal scar that replaces RPE. The MC response was studied by HVEM in 3D computer reconstructions of serial thick sections, made using the STEREC0N program (3), and the HVEM at the NYS Dept. of Health in Albany, NY. Tissue was processed for HVEM or immunofluorescence localization of a monoclonal antibody recognizing MG microvilli (4).

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