Fabrication of a photo-crosslinked gelatin hydrogel for preventing abdominal adhesion

RSC Advances ◽  
2016 ◽  
Vol 6 (95) ◽  
pp. 92449-92453 ◽  
Author(s):  
Wei Wu ◽  
Qing Ni ◽  
Yi Xiang ◽  
Yong Dai ◽  
Su Jiang ◽  
...  
Keyword(s):  
Adhesion Formation ◽  
Gelatin Hydrogel ◽  
Abdominal Adhesion ◽  
Hydrogel Membrane

A photocrosslinkable gelatin-based hydrogel membrane of easy-handling and non-toxic degradation was developed for preventing intra-abdominal adhesion formation.

Honokiol Decreases Intra-Abdominal Adhesion Formation in a Rat Model

2015 ◽  
Vol 79 (3) ◽  
pp. 160-167 ◽  
Author(s):  
Elif Agacayak ◽  
Senem Yaman Tunc ◽  
Mehmet Sait Icen ◽  
Ulas Alabalik ◽  
Fatih Mehmet Findik ◽  
...  
Keyword(s):  
Rat Model ◽  
Adhesion Formation ◽  
Abdominal Adhesion

scholarly journals TMIGD1 Inhibited Abdominal Adhesion Formation by Alleviating Oxidative Stress in the Mitochondria of Peritoneal Mesothelial Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-16
Author(s):  
Yunhua Wu ◽  
Enmeng Li ◽  
Zijun Wang ◽  
Tianli Shen ◽  
Cong Shen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitochondrial Function ◽  
Adhesion Formation ◽  
Mesothelial Cell ◽  
Mesothelial Cells ◽  
Stress Injury ◽  
Peritoneal Mesothelial Cells ◽  
Abdominal Adhesion ◽  
Adhesion Tissue ◽  
Peritoneal Mesothelial Cell

Background. Postoperative abdominal adhesion remains one of the frequent complications after abdominal surgery and lacks effective intervention. Peritoneal mesothelial cell injury and healing play crucial roles in the process of adhesion formation, and identifying this mechanism might provide new insight into possible new therapeutic strategies for this disease. Transmembrane and immunoglobulin domain-containing 1 (TMIGD1) has been proven to protect renal epithelial cells from injury induced by oxidative stress and has also been identified as a novel adhesion molecule. Here, we investigated the role of TMIGD1 and its possible mechanism in adhesion formation. Materials and Methods. Immunohistochemistry (IHC), qPCR, and immunofluorescence (IHF) were used to detect the expression of TMIGD1. The grade and tenacity score of adhesion were used to evaluate the adhesion formation conditions. A TMIGD1-overexpressing HMrSV5 cell line was established. MTT assay, Western blotting, Annexin V apoptosis analysis, and CK19 staining were used to measure mesothelial cell viability, apoptosis, and completeness. ROS and MDA detection were used to measure mesothelial cell oxidative stress levels. JC-1 staining, IHF, and transmission electron microscopy were performed to assess mitochondrial function. Scratch-wound and adhesion assays were used to evaluate the adhesion ability of mesothelial cells. Results. First, we showed that TMIGD1 was decreased in mouse abdominal adhesion tissue and peritoneal mesothelial cells. Second, TMIGD1 overexpression inhibited adhesion formation. Third, TMIGD1 overexpression protected mesothelial cells from hydrogen peroxide- (H2O2-) induced oxidative stress injury. Fourth, TMIGD1 overexpression alleviated oxidative stress by protecting the mitochondrial function of mesothelial cells. In addition, TMIGD1 overexpression enhanced mesothelial cell adhesion. Conclusion. Our findings suggest that TMIGD1 protects mesothelial cells from oxidative stress injury by protecting their mitochondrial function, which is decreased in regular abdominal adhesion tissue. In addition, TMIGD1 enhances peritoneal mesothelial cell adhesion to promote healing.

scholarly journals Preventive Effects of the Intestine Function Recovery Decoction, a Traditional Chinese Medicine, on Postoperative Intra-Abdominal Adhesion Formation in a Rat Model

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Cancan Zhou ◽  
Pengbo Jia ◽  
Zhengdong Jiang ◽  
Ke Chen ◽  
Guanghui Wang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Rat Model ◽  
Adhesion Formation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
High Dose ◽  
Abdominal Adhesion ◽  
Function Recovery ◽  
Model Control

The intestine function recovery decoction (IFRD) is a traditional Chinese medicine that has been used for the treatment of adhesive intestinal obstruction. In this study, the preventative effects and probable mechanism of the IFRD were investigated in a rat model. We randomly assigned rats to five groups: normal, model, control, low dose IFRD, and high dose IFRD. In the animal model, the caecum wall and parietal peritoneum were abraded to induce intra-abdominal adhesion formation. Seven days after surgery, adhesion scores were assessed using a visual scoring system, and histopathological samples were examined. The levels of serum interleukin-6 (IL-6) and transforming growth factor beta-1 (TGF-β1) were analysed by an enzyme-linked immunosorbent assay (ELISA). The results showed that a high dose of IFRD reduced the grade of intra-abdominal adhesion in rats. Furthermore, the grades of inflammation, fibrosis, and neovascularization in the high dose IFRD group were significantly lower than those in the control group. The results indicate that the IFRD can prevent intra-abdominal adhesion formation in a rat model. These data suggest that the IFRD may be an effective antiadhesion agent.

N-acetyl-l-cysteine decreases intra-abdominal adhesion formation through the upregulation of peritoneal fibrinolytic activity and antioxidant defenses

Surgery ◽  
2011 ◽  
Vol 149 (6) ◽  
pp. 801-812 ◽  
Author(s):  
Daniel I. Chu ◽  
Rizal Lim ◽  
Stanley Heydrick ◽  
Melanie L. Gainsbury ◽  
Rami Abdou ◽  
...  
Keyword(s):  
Fibrinolytic Activity ◽  
Adhesion Formation ◽  
Antioxidant Defenses ◽  
Abdominal Adhesion

scholarly journals The effect of rosiglitazone in the prevention of intra-abdominal adhesion formation in a rat uterine horn model

2006 ◽  
Vol 21 (11) ◽  
pp. 3008-3013 ◽  
Author(s):  
F. Demirturk ◽  
H. Aytan ◽  
A. Caliskan ◽  
P. Aytan ◽  
T. Yener ◽  
...  
Keyword(s):  
Adhesion Formation ◽  
Uterine Horn ◽  
Abdominal Adhesion

Saline Hydro-Flotation Is Superior to Bioresorable Membranes in Decreasing Intra-Abdominal Adhesion Formation in a Rat Model

2017 ◽  
Vol 225 (4) ◽  
pp. S90
Author(s):  
John P. Kuckelman ◽  
Morgan Barron ◽  
Kevin Kniery ◽  
Jeffrey Kay ◽  
Joseph P. Kononchik ◽  
...  
Keyword(s):  
Rat Model ◽  
Adhesion Formation ◽  
Abdominal Adhesion

scholarly journals Elucidating the fundamental fibrotic processes driving abdominal adhesion formation

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Deshka S. Foster ◽  
Clement D. Marshall ◽  
Gunsagar S. Gulati ◽  
Malini S. Chinta ◽  
Alan Nguyen ◽  
...  
Keyword(s):  
Adhesion Formation ◽  
Abdominal Adhesion
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