Honokiol Decreases Intra-Abdominal Adhesion Formation in a Rat Model

2015 ◽  
Vol 79 (3) ◽  
pp. 160-167 ◽  
Author(s):  
Elif Agacayak ◽  
Senem Yaman Tunc ◽  
Mehmet Sait Icen ◽  
Ulas Alabalik ◽  
Fatih Mehmet Findik ◽  
...  
Keyword(s):  
Rat Model ◽  
Adhesion Formation ◽  
Abdominal Adhesion

scholarly journals Preventive Effects of the Intestine Function Recovery Decoction, a Traditional Chinese Medicine, on Postoperative Intra-Abdominal Adhesion Formation in a Rat Model

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Cancan Zhou ◽  
Pengbo Jia ◽  
Zhengdong Jiang ◽  
Ke Chen ◽  
Guanghui Wang ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Rat Model ◽  
Adhesion Formation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
High Dose ◽  
Abdominal Adhesion ◽  
Function Recovery ◽  
Model Control

The intestine function recovery decoction (IFRD) is a traditional Chinese medicine that has been used for the treatment of adhesive intestinal obstruction. In this study, the preventative effects and probable mechanism of the IFRD were investigated in a rat model. We randomly assigned rats to five groups: normal, model, control, low dose IFRD, and high dose IFRD. In the animal model, the caecum wall and parietal peritoneum were abraded to induce intra-abdominal adhesion formation. Seven days after surgery, adhesion scores were assessed using a visual scoring system, and histopathological samples were examined. The levels of serum interleukin-6 (IL-6) and transforming growth factor beta-1 (TGF-β1) were analysed by an enzyme-linked immunosorbent assay (ELISA). The results showed that a high dose of IFRD reduced the grade of intra-abdominal adhesion in rats. Furthermore, the grades of inflammation, fibrosis, and neovascularization in the high dose IFRD group were significantly lower than those in the control group. The results indicate that the IFRD can prevent intra-abdominal adhesion formation in a rat model. These data suggest that the IFRD may be an effective antiadhesion agent.

Saline Hydro-Flotation Is Superior to Bioresorable Membranes in Decreasing Intra-Abdominal Adhesion Formation in a Rat Model

2017 ◽  
Vol 225 (4) ◽  
pp. S90
Author(s):  
John P. Kuckelman ◽  
Morgan Barron ◽  
Kevin Kniery ◽  
Jeffrey Kay ◽  
Joseph P. Kononchik ◽  
...  
Keyword(s):  
Rat Model ◽  
Adhesion Formation ◽  
Abdominal Adhesion

scholarly journals Evaluation of surgical anti-adhesion products to reduce postsurgical intra-abdominal adhesion formation in a rat model

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0172088 ◽  
Author(s):  
Long-Xiang Lin ◽  
Fang Yuan ◽  
Hui-Hui Zhang ◽  
Ni-Na Liao ◽  
Jing-Wan Luo ◽  
...  
Keyword(s):  
Rat Model ◽  
Adhesion Formation ◽  
Abdominal Adhesion

Peritoneal cell sheets composed of mesothelial cells and fibroblasts prevent intra-abdominal adhesion formation in a rat model

2013 ◽  
Vol 10 (10) ◽  
pp. 855-866 ◽  
Author(s):  
Kunio Kawanishi ◽  
Masayuki Yamato ◽  
Ryouichi Sakiyama ◽  
Teruo Okano ◽  
Kosaku Nitta
Keyword(s):  
Rat Model ◽  
Adhesion Formation ◽  
Mesothelial Cells ◽  
Peritoneal Cell ◽  
Cell Sheets ◽  
Abdominal Adhesion

scholarly journals Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

2016 ◽  
Vol 39 (1) ◽  
pp. 33-46 ◽  
Author(s):  
Guangbing Wei ◽  
Xin Chen ◽  
Guanghui Wang ◽  
Lin Fan ◽  
Kang Wang ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Rat Model ◽  
Transforming Growth Factor ◽  
Adhesion Formation ◽  
Adhesion Prevention ◽  
Enzyme Linked Immunosorbent Assay ◽  
Abdominal Adhesions ◽  
Abdominal Adhesion ◽  
Tnf Α ◽  
Tgf Β1

Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.

Efficacy of Tenoxicam on Intra-Abdominal Adhesion Prevention in a Rat Model

1996 ◽  
Vol 24 (4) ◽  
pp. 352-357 ◽  
Author(s):  
T Yilmazlar ◽  
E Kaya ◽  
E Gürpinar ◽  
H Emiroğlu
Keyword(s):  
Rat Model ◽  
Abdominal Cavity ◽  
Adhesion Formation ◽  
Test Material ◽  
Adhesion Prevention ◽  
Control Group ◽  
Albino Rats ◽  
Abdominal Adhesion ◽  
Wistar Albino Rats ◽  
The Right

The aim of this study was to investigate the effect of tenoxicam as a non-steroidal anti-inflammatory drug (NSAID) on intra-abdominal adhesion prevention in a rat model. Altogether 50 Wistar-Albino rats weighing 220 – 280 g were assigned to five groups, each of which was made up of 10 rats. All the rats were anaesthetized and prepared for sterile surgery. After a mid-line laparotomy was performed, a 1 cm area of the caecum was rubbed with gauze until subserosal haemorrhage developed, and then a 5 mm-diameter part of the peritoneum on the right side of the abdominal wall was removed. Prior to complete closure, 3 ml of the test material was placed into the abdominal cavity. On the eighth day the rats were killed and the adhesion score was determined. The groups and their mean adhesion scores were as follows: control group (normal saline), 2.5; group of dilution buffer, 1.8; tenoxicam (0.125 mg/kg), 1.3; tenoxicam (0.25 mg/kg), 1.3; and tenoxicam (0.5 mg/kg), 0.9. The differences between the adhesion scores among all the groups ( P < 0.05, Kruskal-Wallis test), and those between the tenoxicam groups and control group ( P < 0.05, Mann-Whitney U-test), were significant. Thus a single instillation of tenoxicam into the peritoneal cavity at the time of surgery reduced adhesion formation effectively in this model, irrespective of dosage.

Sign in / Sign up

Export Citation Format

Share Document