scholarly journals Vapor-based micro/nano-partitioning of fluoro-functional group immobilization for long-term stable cell patterning

RSC Advances ◽  
2016 ◽  
Vol 6 (98) ◽  
pp. 96306-96313 ◽  
Author(s):  
Shun-ichi Funano ◽  
Nobuyuki Tanaka ◽  
Yo Tanaka
Keyword(s):  
Cell Culture ◽  
Functional Group ◽  
Cell Patterning ◽  
Nano Scale ◽  
A Cell ◽  
Culture Surface ◽  
Immobilization Method

This study developed a simple vapor-based immobilization method using a compound with fluoro-functional-group on a cell culture surface with micro/nano scale patterns.

scholarly journals Vacuum microcasting of 2-methacryloyloxyethyl phosphorylcholine polymer for stable cell patterning

BioTechniques ◽  
2020 ◽  
Vol 69 (3) ◽  
pp. 171-177
Author(s):  
Nobuyuki Tanaka ◽  
Ryoji Sekine ◽  
Shun-ichi Funano ◽  
Asako Sato ◽  
Núria Taberner Carretero ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Adhesion ◽  
Polymer Film ◽  
Cell Patterning ◽  
Ethanol Solution ◽  
Rapid Fabrication ◽  
A Cell ◽  
Culture Surface

This study demonstrates the rapid fabrication and utility of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer film for cell patterning. The film was obtained on a cell culture surface by microcasting MPC polymer ethanol solution into a degassed polydimethylsiloxane mold with a desired pattern. After removal of the mold, 293AD cells were cultured on the surface of the polymer film with the patterned microstructures. Patterned cell adhesion restricted by the film was successfully maintained during at least a 168-h cultivation. The microcast MPC polymer film can be prepared rapidly and used for efficient long-term cell confinement.

scholarly journals A cell culture platform for Cryptosporidium that enables long-term cultivation and new tools for the systematic investigation of its biology

2018 ◽  
Vol 48 (3-4) ◽  
pp. 197-201 ◽  
Author(s):  
Christopher N. Miller ◽  
Lyne Jossé ◽  
Ian Brown ◽  
Ben Blakeman ◽  
Jane Povey ◽  
...  
Keyword(s):  
Cell Culture ◽  
Systematic Investigation ◽  
A Cell

scholarly journals A cell culture platform forcryptosporidiumthat enables long-term cultivation and new tools for the systematic investigation of its biology

2017 ◽  
Author(s):  
Christopher N. Miller ◽  
Lyne Jossé ◽  
Ian Brown ◽  
Ben Blakeman ◽  
Jane Povey ◽  
...  
Keyword(s):  
Cell Culture ◽  
Systematic Investigation ◽  
Dapi Staining ◽  
Specific Staining ◽  
Vicia Villosa ◽  
Life Cycle Stages ◽  
Vicia Villosa Lectin ◽  
A Cell ◽  
Thin Walled

AbstractCryptosporidiumparasites are a major cause of diarrhoea that pose a particular threat to children in developing areas and immunocompromised individuals. Curative therapies and vaccines are lacking. Currently,Cryptosporidiumoocysts for research must be freshly produced in animals and cannot be long-term stored. Here, we show that COLO-680N cells infected with two differentCryptosporidium parvumstrains (Moredun, Iowa) produce sufficient infectious oocysts to infect subsequent cultures. Oocyst identity was confirmed by specific staining (Crypt-a-glo, Vicia Villosa lectin, Sporo-glo), PCR-based amplification ofCryptosporidium-specific genes, lipidomics fingerprinting, and atomic force microscopy (AFM). Antibody-stained oocysts produced unstained oocysts confirming production of novel oocysts. Infected cultures could be cryoconserved and continued to produce infectious oocysts after resuscitation. Transmission electron microscopy identified all keyCryptosporidiumlife cycle stages. Infected cultures produced thick-walled (primarily involved inCryptosporidiumtransmission between organisms) and thin-walled oocysts (important forCryptosporidiumpropagation within a host/tissue) as indicated by DAPI staining (only thin-walled oocysts are permeable to DAPI staining, thus allowing visualisation of sporozoites) and AFM. In conclusion, we present a novel, easy-to-handle cell culture system that enables the propagation, cryopreservation and detailed investigation ofCryptosporidiumat a laboratory scale. Its availability will accelerate research onCryptosporidiumand the development of anti-Cryptosporidiumdrugs.

A cell-culture system for long-term maintenance of elevated hydrostatic pressure with the option of additional tension

2002 ◽  
Vol 35 (5) ◽  
pp. 579-584 ◽  
Author(s):  
Cornelia Hasel ◽  
Susanne Dürr ◽  
Silke Brüderlein ◽  
Ingo Melzner ◽  
Peter Möller
Keyword(s):  
Cell Culture ◽  
Hydrostatic Pressure ◽  
Culture System ◽  
Cell Culture System ◽  
Elevated Hydrostatic Pressure ◽  
A Cell ◽  
Term Maintenance
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