Determining the substrate permeability through the bilayer of large unilamellar vesicles of DOPC. A kinetic study

RSC Advances ◽  
2016 ◽  
Vol 6 (67) ◽  
pp. 62594-62601 ◽  
Author(s):  
Maria Alejandra Luna ◽  
Juana J. Silber ◽  
Leonides Sereno ◽  
N. Mariano Correa ◽  
Fernando Moyano
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Study ◽  
Unilamellar Vesicles ◽  
Large Unilamellar Vesicles ◽  
Hydrolysis Of

In this work we determine the permeability of DOPC vesicles in the presence of different cholesterol contents, by using the enzymatic hydrolysis of N-benzoyl-l-tyrosine p-nitroanilide catalyzed by α-chymotrypsin.

A kinetic study on enzymatic hydrolysis of a variety of pulps for its enhancement with continuous ultrasonic irradiation

2004 ◽  
Vol 19 (2) ◽  
pp. 155-164 ◽  
Author(s):  
Chengzhou Li ◽  
Makoto Yoshimoto ◽  
Naoki Tsukuda ◽  
Kimitoshi Fukunaga ◽  
Katsumi Nakao
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Study ◽  
Ultrasonic Irradiation ◽  
Hydrolysis Of

Enzymatic hydrolysis of cellulosic materials: A kinetic study

1984 ◽  
Vol 26 (10) ◽  
pp. 1233-1238 ◽  
Author(s):  
Pier Luigi Beltrame ◽  
Paolo Carniti ◽  
Bonaventura Focher ◽  
Annamaria Marzetti ◽  
Virginio Sarto
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Study ◽  
Cellulosic Materials ◽  
Hydrolysis Of

Kinetic study of enzymatic hydrolysis of starch isolated from sorghum grain cultivars by various methods

2013 ◽  
Vol 52 (1) ◽  
pp. 451-457
Author(s):  
Rachid Souilah ◽  
Nadia Boudries ◽  
Djaffar Djabali ◽  
Badreddine Belhadi ◽  
Boubekeur Nadjemi
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Study ◽  
Sorghum Grain ◽  
Hydrolysis Of

A KINETIC STUDY OF ENZYMATIC HYDROLYSIS OF OIL PALM BIOMASS FOR FERMENTABLE SUGAR USING POLYETHYLENE GLYCOL (PEG) IMMOBILIZED CELLULASE

2016 ◽  
Vol 78 (8-3) ◽  
Author(s):  
Umi Aisah Asli ◽  
Isah Nwaha ◽  
Hazirah Hamid ◽  
Zainul Akmar Zakaria ◽  
Aziatul Niza Sadikin ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Enzymatic Hydrolysis ◽  
Kinetic Study ◽  
Oil Palm ◽  
Fermentable Sugar ◽  
Michaelis Constant ◽  
Immobilized Cellulase ◽  
Free Interaction ◽  
Limiting Velocity ◽  
Hydrolysis Of

In this work, enzymatic hydrolysis by cellulase in a soluble and an immobilized form was studied to convert lignocellulosic oil palm empty fruit bunch (EFB) biomass into fermentable sugars as a feedstock for bioethanol production. The cellulase was covalently immobilized with activated and functionalized polyethylene glycol (PEG) via glutaraldehyde coupling method. As a whole, the immobilized cellulase displayed 50% higher efficiency over free cellulase, in reducing sugar recovery during hydrolysis reactions at pH of 4.8 and temperature of 50°C. From the kinetic study, it showed that Michaelis constant (Km) and limiting velocity (Vmax) of immobilized cellulase were 179.2 mg/ml and 33.5 mg/ml.min respectively, comparable with the value for free cellulose, 171.8 mg/ml and 34.5 mg/ml.min respectively. This result could be attributed to the effect of PEG on the binding cellulase to substrate desorb substrates, and enables free interaction of cellulase to hydrolyse cellulose maximally

scholarly journals Kinetic study of the enzymatic hydrolysis of sugarcane bagasse

2013 ◽  
Vol 30 (3) ◽  
pp. 437-447 ◽  
Author(s):  
M. L. Carvalho ◽  
R. Sousa Jr. ◽  
U. F. Rodríguez-Zúñiga ◽  
C. A. G. Suarez ◽  
D. S. Rodrigues ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Study ◽  
Sugarcane Bagasse ◽  
Hydrolysis Of
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