Charged surface groups of nanoparticles and the adsorbed proteins codetermine the fate of nanoparticles upon interacting with cells

RSC Advances ◽  
2016 ◽  
Vol 6 (63) ◽  
pp. 58315-58324 ◽  
Author(s):  
Ke Huang ◽  
Yang Hu ◽  
Changjun Yu ◽  
Rena Boerhan ◽  
Guoqiang Jiang
Keyword(s):  
Intracellular Transport ◽  
Protein Corona ◽  
Surface Groups ◽  
Charged Surface ◽  
P Protein ◽  
Adsorbed Proteins

Protein corona had impact on the endocytosis mechanism and then the intracellular transport of NPs, especially the exocytosis.

scholarly journals Visualization of Intracellular Transport of Vesicular Stomatitis Virus Nucleocapsids in Living Cells

2006 ◽  
Vol 80 (13) ◽  
pp. 6368-6377 ◽  
Author(s):  
Subash C. Das ◽  
Debasis Nayak ◽  
You Zhou ◽  
Asit K. Pattnaik
Keyword(s):  
Vesicular Stomatitis Virus ◽  
Protein Function ◽  
Intracellular Transport ◽  
Fluorescent Protein ◽  
De Novo ◽  
Virus Production ◽  
Hinge Region ◽  
Cell Periphery ◽  
P Protein ◽  
Vesicular Stomatitis

ABSTRACT The phosphoprotein (P) of vesicular stomatitis virus (VSV) is a subunit of the viral RNA polymerase. In previous studies, we demonstrated that insertion of 19 amino acids in the hinge region of the protein had no significant effect on P protein function. In the present study, we inserted full-length enhanced green fluorescent protein (eGFP) in frame into the hinge region of P and show that the fusion protein (PeGFP) is functional in viral genome transcription and replication, albeit with reduced activity. A recombinant vesicular stomatitis virus encoding PeGFP in place of the P protein (VSV-PeGFP), which possessed reduced growth kinetics compared to the wild-type VSV, was recovered. Using the recombinant VSV-PeGFP, we show that the viral replication proteins and the de novo-synthesized RNA colocalize to sites throughout the cytoplasm, indicating that replication and transcription are not confined to any particular region of the cytoplasm. Real-time imaging of the cells infected with the eGFP-tagged virus revealed that, following synthesis, the nucleocapsids are transported toward the cell periphery via a microtubule (MT)-mediated process, and the nucleocapsids were seen to be closely associated with mitochondria. Treatment of cells with nocodazole or Colcemid, drugs known to inhibit MT polymerization, resulted in accumulation of the nucleocapsids around the nucleus and also led to inhibition of infectious-virus production. These findings are compatible with a model in which the progeny viral nucleocapsids are transported toward the cell periphery by MT and the transport may be facilitated by mitochondria.

Disease-specific protein corona sensor arrays may have disease detection capacity

2019 ◽  
Vol 4 (5) ◽  
pp. 1063-1076 ◽  
Author(s):  
Giulio Caracciolo ◽  
Reihaneh Safavi-Sohi ◽  
Reza Malekzadeh ◽  
Hossein Poustchi ◽  
Mahdi Vasighi ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
Sensor Array ◽  
Sensor Arrays ◽  
Protein Corona ◽  
Specific Protein ◽  
Disease Detection ◽  
P Protein ◽  
Array Technology ◽  
Disease Specific

Protein corona sensor array technology identifies diseases through specific proteomics pattern recognition.

Protein corona profile of graphene oxide allows detection of glioblastoma multiforme using a simple one-dimensional gel electrophoresis technique: a proof-of-concept study

2021 ◽  
Author(s):  
Riccardo Di Santo ◽  
Erica Quagliarini ◽  
Luca Digiacomo ◽  
Daniela Pozzi ◽  
Angelina Di Carlo ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Glioblastoma Multiforme ◽  
Gel Electrophoresis ◽  
Blood Test ◽  
Protein Corona ◽  
Proof Of Concept ◽  
One Dimensional ◽  
Concept Study ◽  
P Protein ◽  
Electrophoresis Technique

Protein Corona-based blood test for the detection of glioblastoma multiforme.

scholarly journals Protein source and choice of anticoagulant decisively affect nanoparticle protein corona and cellular uptake

Nanoscale ◽  
2016 ◽  
Vol 8 (10) ◽  
pp. 5526-5536 ◽  
Author(s):  
S. Schöttler ◽  
Katja Klein ◽  
K. Landfester ◽  
V. Mailänder
Keyword(s):  
Human Plasma ◽  
Cellular Uptake ◽  
Protein Corona ◽  
Protein Source ◽  
Protein Sources ◽  
P Protein

Protein corona investigations use different protein sources. Outcome of investigations is crucial. Human plasma seems to be a better choice than serum from animals or also human.

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