scholarly journals Protein source and choice of anticoagulant decisively affect nanoparticle protein corona and cellular uptake

Nanoscale ◽  
2016 ◽  
Vol 8 (10) ◽  
pp. 5526-5536 ◽  
Author(s):  
S. Schöttler ◽  
Katja Klein ◽  
K. Landfester ◽  
V. Mailänder
Keyword(s):  
Human Plasma ◽  
Cellular Uptake ◽  
Protein Corona ◽  
Protein Source ◽  
Protein Sources ◽  
P Protein

Protein corona investigations use different protein sources. Outcome of investigations is crucial. Human plasma seems to be a better choice than serum from animals or also human.

Protein corona formation and its influence on biomimetic magnetite nanoparticles

2020 ◽  
Vol 8 (22) ◽  
pp. 4870-4882 ◽  
Author(s):  
Ana Peigneux ◽  
Emanuel A. Glitscher ◽  
Rawan Charbaji ◽  
Christoph Weise ◽  
Stefanie Wedepohl ◽  
...  
Keyword(s):  
Human Plasma ◽  
Cellular Uptake ◽  
Magnetite Nanoparticles ◽  
Colloidal Stability ◽  
Protein Corona ◽  
Corona Formation

Colloidal stability and cellular uptake of MamC-biomimetic magnetite nanoparticles (BMNPs) incubated with human plasma (PC-BMNPs).

Effects of protein source, formaldehyde treatment and rumen-protected methionine on the metabolism and performance of pregnant and lactating ewes fed straw

2001 ◽  
Vol 2001 ◽  
pp. 54-54
Author(s):  
D. Handford ◽  
S.E. Pattinson ◽  
R.G. Wilkinson ◽  
L.A. Sinclair
Keyword(s):  
Vegetable Protein ◽  
Soya Bean ◽  
Protein Source ◽  
Current Experiment ◽  
Biological Value ◽  
Protein Sources ◽  
Formaldehyde Treatment ◽  
Bean Meal ◽  
And Performance ◽  
Soya Bean Meal

Fishmeal is a suitable protein source for pregnant and lactating ewes, providing higher levels of undegradable protein than vegetable protein sources, with an improved biological value (Robinson, 1987). Vegetable protein sources may however be improved by formaldehyde treatment to reduce protein degradability and by the addition of rumen-protected amino acids. The objective of the current experiment was to compare the effects of feeding concentrates containing fishmeal with concentrates containing soya-bean meal, formaldehyde treated soya-bean meal and formaldehyde treated soya-bean meal with rumen-protected methionine.

scholarly journals Nano-Bio Interaction between Blood Plasma Proteins and Water-Soluble Silicon Quantum Dots with Enabled Cellular Uptake and Minimal Cytotoxicity

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2250
Author(s):  
Shanmugavel Chinnathambi ◽  
Nobutaka Hanagata ◽  
Tomohiko Yamazaki ◽  
Naoto Shirahata
Keyword(s):  
Quantum Dots ◽  
Blood Plasma ◽  
Cellular Uptake ◽  
Plasma Proteins ◽  
Protein Corona ◽  
Water Soluble ◽  
Pluronic F127 ◽  
Infrared Light ◽  
Spectral Window ◽  
Blood Plasma Proteins

A better understanding of the compatibility of water-soluble semiconductor quantum dots (QDs) upon contact with the bloodstream is important for biological applications, including biomarkers working in the first therapeutic spectral window for deep tissue imaging. Herein, we investigated the conformational changes of blood plasma proteins during the interaction with near-infrared light-emitting nanoparticles, consisting of Pluronic F127 shells and cores comprised of assembled silicon QDs terminated with decane monolayers. Albumin and transferrin have high quenching constants and form a hard protein corona on the nanoparticle. In contrast, fibrinogen has low quenching constants and forms a soft protein corona. A circular dichroism (CD) spectrometric study investigates changes in the protein’s secondary and tertiary structures with incremental changes in the nanoparticle concentrations. As expected, the addition of nanoparticles causes the denaturation of the plasma proteins. However, it is noteworthy that the conformational recovery phenomena are observed for fibrinogen and transferrin, suggesting that the nanoparticle does not influence the ordered structure of proteins in the bloodstream. In addition, we observed enabled cellular uptake (NIH3T3 Fibroblasts) and minimal cytotoxicity using different cell lines (HeLa, A549, and NIH3T3). This study offers a basis to design QDs without altering the biomacromolecule’s original conformation with enabled cellular uptake with minimal cytotoxicity.

Surface roughness influences the protein corona formation of glycosylated nanoparticles and alter their cellular uptake

Nanoscale ◽  
2019 ◽  
Vol 11 (48) ◽  
pp. 23259-23267 ◽  
Author(s):  
Alberto Piloni ◽  
Chin Ken Wong ◽  
Fan Chen ◽  
Megan Lord ◽  
Andreas Walther ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Cellular Uptake ◽  
Protein Corona ◽  
Biological Behavior ◽  
Protein Absorption ◽  
Corona Formation

Patterned nanoparticle surfaces can repel protein absorption and prevent the formation of a protein corona, which alters the biological behavior and therefore the fate of the nanoparticle.

Disease-specific protein corona sensor arrays may have disease detection capacity

2019 ◽  
Vol 4 (5) ◽  
pp. 1063-1076 ◽  
Author(s):  
Giulio Caracciolo ◽  
Reihaneh Safavi-Sohi ◽  
Reza Malekzadeh ◽  
Hossein Poustchi ◽  
Mahdi Vasighi ◽  
...  
Keyword(s):  
Pattern Recognition ◽  
Sensor Array ◽  
Sensor Arrays ◽  
Protein Corona ◽  
Specific Protein ◽  
Disease Detection ◽  
P Protein ◽  
Array Technology ◽  
Disease Specific

Protein corona sensor array technology identifies diseases through specific proteomics pattern recognition.

scholarly journals Plasma proteins facilitates placental transfer of polystyrene particles

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Michael M. Gruber ◽  
Birgit Hirschmugl ◽  
Natascha Berger ◽  
Magdalena Holter ◽  
Snježana Radulović ◽  
...  
Keyword(s):  
Human Plasma ◽  
Human Placenta ◽  
Plasma Proteins ◽  
Placental Transfer ◽  
Ex Vivo ◽  
Biological Fluids ◽  
Protein Corona ◽  
Human Albumin ◽  
Placental Perfusion ◽  
The Impact

Abstract Background Nanoparticles, which are exposed to biological fluids are rapidly interacting with proteins and other biomolecules forming a corona. In addition to dimension, charge and material the distinct protein corona influences the interplay of nanoparticles with tissue barriers. In this study we were focused on the impact of in situ formed human plasma protein corona on the transfer of 80 nm polystyrene nanoparticles (PS-particles) across the human placenta. To study materno-to fetal PS transfer we used the human ex vivo placental perfusion approach, which represents an intact and physiological tissue barrier. To analyze the protein corona of PS particles we performed shotgun proteomics of isolated nanoparticles before and after tissue exposure. Results Human plasma incubated with PS-particles of 80 nm and subsequent formed protein corona enhanced the transfer across the human placenta compared to PS-corona formed by bovine serum albumin and dextran which served as a control. Quantitative and qualitative changes of plasma proteins determined the changes in PS transfer across the barrier. Based on the analysis of the PS-proteome two candidate proteins, namely human albumin and immunoglobulin G were tested if these proteins may account for the enhanced PS-transfer across the placenta. Interestingly, the protein corona formed by human albumin significantly induced the transfer of PS-particles across the tissue compared to the formed IgG-corona. Conclusion In total we demonstrate the PS corona dynamically and significantly evolves upon crossing the human placenta. Thus, the initial composition of PS particles in the maternal circulation is not predictive for their transfer characteristics and performance once beyond the barrier of the placenta. The precise mechanism of these effects remains to be elucidated but highlights the importance of using well designed biological models when testing nanoparticles for biomedical applications.

Preparation of the protein corona: How washing shapes the proteome and influences cellular uptake of nanocarriers

2020 ◽  
Vol 114 ◽  
pp. 333-342
Author(s):  
Maximilian Brückner ◽  
Johanna Simon ◽  
Shuai Jiang ◽  
Katharina Landfester ◽  
Volker Mailänder
Keyword(s):  
Cellular Uptake ◽  
Protein Corona

scholarly journals Comparative study on the effects of low cost oil seed cakes and fish meal as dietary protein sources for Labeo rohita (Hamilton) fingerling

1970 ◽  
Vol 27 ◽  
pp. 25-30 ◽  
Author(s):  
KA Latif ◽  
MT Alam ◽  
MA Sayeed ◽  
M Afzal Hussain ◽  
S Sultana ◽  
...  
Keyword(s):  
Dietary Protein ◽  
Fish Meal ◽  
Labeo Rohita ◽  
Low Cost ◽  
Protein Source ◽  
Economic Point ◽  
Protein Sources ◽  
Oil Seed ◽  
Dietary Fish ◽  
Oil Seed Cakes

A trial was conducted to evaluate the effects of combination of soybean meal (SBN), mustard oilcake (MOC), sesame oilcake (SOC) and linseed oilcake (LOC) as alternative for dietary fish meal (FM) protein for Labeo rohita fingerling for a period of 56 days. Six experimental diets were formulated by replacing 30, 50, 60, 70 and 100% (2 diets) of the total dietary fish meal protein by the combination of FM, SBM, MOC, SOC, and LOC. The combinations of the protein sources were as diet 1 (FM 100%), diet 2 (FM:SBM:MOC::70:15:15), diet 3 (FM:SBM:SOC:LOC::50:20:20:10), diet 4 (FM:SBM:SOC:LOC::40:20:20:20), diet 5 (FM:SBM:SOC:LOC::25:30:15:30), diet 6 (SBM:MOC:SOC:LOC::25:25:25:25) and diet 7 (SBM:SOC:LOC::40:30:30). The performances of these six test diets were compared with the diet 1 (control). All diets were iso-nitrogenous containing about 30% protein. The growth trial was conducted in a flow through system under laboratory condition with three replications for each treatment. On the basis of the growth response, food conversion ratio (FCR), protein efficiency ratio (PER) diet 7 showed better performance followed by diet 1, diet 2, diet 5, diet 3, diet 4 and diet 6 respectively but those were not significantly different (P>0.05). The diet 6 produced the lowest performance. The apparent protein digestibility (APD) values for all the experimental diets were very nearer but significantly different (P0.05) in diets even from control one which were formulated to evaluate the effects of combination of low cost oil seed cakes as dietary protein source for Labeo rohita fingerling. Hence, it might be concluded that from the economic point of view, that all the low cost oil seed cakes which are locally available can be used in combination form as the alternative protein source for the feed of Labeo rohita. Key words: Oil seed cakes, Labeo rohita, fingerling, protein source.   doi:10.3329/ujzru.v27i0.1949 Univ. j. zool. Rajshahi Univ. Vol. 27, 2008 pp. 25-30

scholarly journals Cellular uptake of ribonuclease A-functionalised core–shell silica microspheres

2014 ◽  
Vol 2 (42) ◽  
pp. 7307-7315 ◽  
Author(s):  
G. F. Chimonides ◽  
J. M. Behrendt ◽  
E. Chundoo ◽  
C. Bland ◽  
A. V. Hine ◽  
...  
Keyword(s):  
Cell Viability ◽  
Cellular Uptake ◽  
Ribonuclease A ◽  
Core Shell ◽  
Protein Transduction ◽  
Silica Microspheres ◽  
P Protein

Protein transduction: core–shell microspheres have been synthesised and coupled to ribonuclease A. Cellular uptake of these microspheres causes significantly reduced levels of intracellular RNA and reduced cell viability demonstrating that core–shell microsphere-mediated delivery of active enzymes into cells is effective.

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