Evaluation of the antimicrobial activity of Moringa oleifera seed extract as a sustainable solution for potable water

RSC Advances ◽  
2016 ◽  
Vol 6 (31) ◽  
pp. 25918-25926 ◽  
Author(s):  
Saumyadeb Dasgupta ◽  
Naga Siva Kumar Gunda ◽  
Sushanta K. Mitra
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Moringa Oleifera ◽  
Potable Water ◽  
Seed Extract ◽  
Antimicrobial Study ◽  
Moringa Oleifera Seed

We have provided a detailed antimicrobial study of the seed extract ofMoringa oleifera, a common medicinal plant.

scholarly journals 70 Effects of the addition of Moringa oleifera seed extract to in vitro maturation of bovine oocyte media and its antimicrobial activity

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 3-3
Author(s):  
Theisy P Acosta Pérez
Keyword(s):  
Antimicrobial Activity ◽  
In Vitro Maturation ◽  
Moringa Oleifera ◽  
Cumulus Cells ◽  
Seed Extract ◽  
Chi Square ◽  
Cumulus Expansion ◽  
Conventional Antibiotics ◽  
Moringa Oleifera Seed

Abstract Moringa oleifera seed extract was added to the in vitro maturation media of bovine oocytes as an alternative antibiotic to evaluate its effect on the oocyte maturation and its antimicrobial activity. Oocytes (n = 405) were recovered from ovaries from slaughtered cows, classified by quality and divided in four categories according to the cytoplasm appearance and cumulus cells layers. Oocytes were washed four times in TCM-199 supplemented with fetal bovine serum (FBS) and FSH, then placed in maturation media, which contained TCM-199 supplemented with FBS, FSH and the corresponding treatments: TCM-199+gentamicin (Control), TCM-199 without antibiotic (Test), moringa (M) 1.0, 5.0 and 10.0 mg/mL. After 22 h at 38.5°C in a 5% CO2 humidified atmosphere, oocytes were examined to determine cumulus expansion (as categorical results yes or no) and cumulus expansion Index (CEI). Additionally, 400 µL of used maturation media was retrieved and stored for the antimicrobial activity tests in agar cultures. Evaluation of the cultures for colony-forming units (CFU) was done at 24 and 48 h post culture. Results were analyzed with Chi Square test (for expansion rates) and an ANOVA (for the CEI and CFU) using the SAS system, data are presented as mean ± standard error. Statistically, there was no difference between treatments (P >0.05). Numerically M 1.0 and Test obtained the highest maturation rate (96.43%; 96.20%). M 1.0 and Control showed the highest CEI (2.26±0.21; 2.25±0.26). For the CFU count, Control showed the lowest result at 24 and 48 h post culture (0.00 ± 0.00; 0.11 ± 0.33), as M 10.0 showed the highest result at both 24 and 48 h (2.00 ± 3.60; 2.55 ± 4.58). The addition M 1.0 mg/mL to the maturation media is an alternative for the use of conventional antibiotics without decreasing maturation rates.

scholarly journals Application of Moringa Oleifera seed extract to treat coffee fermentation wastewater

2017 ◽  
Vol 329 ◽  
pp. 102-109 ◽  
Author(s):  
William K. Garde ◽  
Steven G. Buchberger ◽  
David Wendell ◽  
Margaret J. Kupferle
Keyword(s):  
Moringa Oleifera ◽  
Seed Extract ◽  
Moringa Oleifera Seed

scholarly journals Effect of Moringa oleifera seed extract on antioxidant activity and sperm characteristics in cryopreserved ram semen

2020 ◽  
Vol 48 (1) ◽  
pp. 114-120
Author(s):  
José Maria Carrera-Chávez ◽  
Edson Eduardo Jiménez-Aguilar ◽  
Theisy Patricia Acosta-Pérez ◽  
José Alberto Núñez-Gastélum ◽  
Andrés Quezada-Casasola ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Moringa Oleifera ◽  
Seed Extract ◽  
Sperm Characteristics ◽  
Moringa Oleifera Seed

scholarly journals Identification and Purification of Potential Bioactive Peptide of Moringa oleifera Seed Extracts

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1445
Author(s):  
Sangeeta Chandrashekar ◽  
Raman Vijayakumar ◽  
Ramachandran Chelliah ◽  
Deog-Hwan Oh
Keyword(s):  
Moringa Oleifera ◽  
Anticoagulant Activity ◽  
Cell Aggregation ◽  
Seed Extract ◽  
Amino Acid Sequences ◽  
Bioactive Peptide ◽  
Liquid Chromatography Mass Spectrometry ◽  
Bacterial Strains ◽  
Moringa Oleifera Seed ◽  
Seed Extracts

The aim of the study was to investigate the antibacterial and anticoagulant activity of Moringa (Moringa oleifera) seed extracts and coagulant protein for their potential application in water treatment. Pathogenic microorganisms were obtained from Ramachandra Hospital, Chennai, India. Bacterial cell aggregation and growth kinetics studies were employed for six bacterial strains with different concentrations of seed extracts and coagulant protein. Moringa seed extract and coagulant protein showed cell aggregation against six bacterial strains, whereas seed extract alone showed growth inhibition of all six bacterial strains for up to 6 h, compared to that of control. Escherichia coli and Salmonella para typhi B did not develop resistance against coagulant protein. The results imply that Moringa oleifera is likely an efficient low-molecular bioactive peptide (with <7.5 kDa plant-based coagulant and antimicrobial peptides, confirmed by applying amino acid sequences), using liquid chromatography–mass spectrometry and HPLC, with the corresponding sequences from Napin-1A peptide posing different degrees of antibacterial activity against different pathogenic organisms.

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