Antibacterial and immunomodulatory activity of moringa ( Moringa oleifera ) seed extract in Longfin yellowtail ( Seriola rivoliana ) peripheral blood leukocytes

Background CD24 is a sialoglycoprotein anchored to the cell surface via glycosylphosphatidylinositol and is involved in intracellular signaling processes. It plays an important role in the early stages of the multistep process of colorectal carcinogenesis. Several single nucleotide polymorphisms in the CD24 gene are reported to exert a diverse effect on cancer risk. We aimed to elucidate whether CD24 TG/del genetic variants are associated with susceptibility to colorectal cancer (CRC). Methods The study included 179 subjects, 36 with CRC (prior to surgery) and 143 healthy control subjects. Deoxyribonucleic acid was purified from peripheral blood leukocytes, and by using restriction fragment length polymorphism analysis, the CD24 gene was genotyped for the specific genetic variant, TG deletion. Additionally, CD24 protein expression levels were determined by Western blotting analysis. Results The incidence of the TG/del was higher among the CRC patients compared with healthy controls, 14% and 10%, respectively ( P = .54). CD24 protein levels were significantly higher among CRC patients. There were no significant differences in CD24 expression between CRC patients at different stages of the disease or between patients who carry the mutation and those who did not. Conclusions CD24 genetic variant might be of clinical value for risk assessment as part of cancer prevention programs. Further study on larger populations is needed to validate the importance of this dinucleotide deletion in CRC development. Overexpression of CD24 protein occurs early along the multistep process of CRC carcinogenesis, and a simple blood sample based on CD24 expression on peripheral blood leukocytes can contribute to early diagnosis.

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Immune gene expression in cyprinid herpesvirus-2 (CyHV-2)–sensitized peripheral blood leukocytes (PBLs) co-cultured with CyHV-2-infected goldfish fin cell line

Aquaculture International ◽

10.1007/s10499-021-00721-6 ◽

2021 ◽

Author(s):

Sweta Das ◽

Arathi Dharmaratnam ◽

Charan Ravi ◽

Raj Kumar ◽

Thangaraj Raja Swaminathan

Keyword(s):

Gene Expression ◽

Cell Line ◽

Peripheral Blood ◽

Immune Gene ◽

Peripheral Blood Leukocytes ◽

Blood Leukocytes ◽

Immune Gene Expression

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The Fruiting Bodies, Submerged Culture Biomass, and Acidic Polysaccharide Glucuronoxylomannan of Yellow Brain Mushroom Tremella mesenterica Modulate the Immunity of Peripheral Blood Leukocytes and Splenocytes in Rats with Impaired Glucose Tolerance

Journal of Traditional and Complementary Medicine ◽

10.4103/2225-4110.124347 ◽

2014 ◽

Vol 4 (1) ◽

pp. 56-63 ◽

Cited By ~ 4

Author(s):

Tai-Hao Hsu ◽

Chien-Hsing Lee ◽

Fang-Yi Lin ◽

Solomon P. Wasser ◽

Hui-Chen Lo

Keyword(s):

Glucose Tolerance ◽

Impaired Glucose Tolerance ◽

Peripheral Blood ◽

Submerged Culture ◽

Fruiting Bodies ◽

Peripheral Blood Leukocytes ◽

Blood Leukocytes ◽

Acidic Polysaccharide

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Lactarane Type Sesquiterpenoids as Inhibitors of Leukotriene Biosynthesis and Other, New Metabolites from Submerged Cultures of Lentinellus cochleatus (Pers. ex Fr.) Karst

Zeitschrift für Naturforschung C ◽

10.1515/znc-1996-7-807 ◽

1996 ◽

Vol 51 (7-8) ◽

pp. 493-499 ◽

Cited By ~ 8

Author(s):

Annette Wunder ◽

Timm Anke ◽

Dörte Klostermeyer ◽

Wolfgang Steglich

Keyword(s):

Peripheral Blood ◽

Human Peripheral Blood ◽

Peripheral Blood Leukocytes ◽

Submerged Cultures ◽

Blood Leukocytes ◽

Spectroscopic Investigations ◽

Basophilic Leukemia ◽

New Metabolites

Abstract Three known sesquiterpenoids of the lactarane and secolactarane type, deoxylactarorufin A (1), blennin A (2) and blennin C (3), have been obtained from cultures of Lentinellus cochleatus (Basidiomycetes) together with the new metabolites (Z)-2-chloro-3-(4-me-thoxyphenyl)-2-propen-l-ol (4) and lentinellone (5), a protoilludane derivative. The structures were determined by spectroscopic investigations. 1, 2 and 3 are potent inhibitors of leukotriene biosynthesis in rat basophilic leukemia (RBL-1) cells and human peripheral blood leukocytes (PBL).

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