scholarly journals Distinguishing binding modes of a new phosphonium dye with DNA by surface-enhanced Raman spectroscopy

RSC Advances ◽  
2016 ◽  
Vol 6 (48) ◽  
pp. 41927-41936 ◽  
Author(s):  
Snežana Miljanić ◽  
Adriana Kenđel ◽  
Morana Novak ◽  
Todor G. Deliqeorqiev ◽  
Ivo Crnolatac ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Structural Data ◽  
Minor Groove ◽  
Surface Enhanced Raman Spectroscopy ◽  
Binding Modes ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Adenine Thymine

SERS spectroscopy provided structural data on binding of a dye as monomer and dimer within minor groove of adenine–thymine polynucleotides.

Cooperative hydrogen-bonding of the adenine–thymine pair as a strategy for lowering the limit of detection of thymine by surface-enhanced Raman spectroscopy

The Analyst ◽  
2016 ◽  
Vol 141 (11) ◽  
pp. 3428-3436 ◽  
Author(s):  
Mónica B. Mamián-López ◽  
Paola Corio ◽  
Marcia L. A. Temperini
Keyword(s):  
Raman Spectroscopy ◽  
Hydrogen Bonding ◽  
Hydrogen Bonds ◽  
Limit Of Detection ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Thymine Adenine ◽  
Cooperative Hydrogen Bonding ◽  
Adenine Thymine

The formation of cooperative hydrogen bonds between the thymine–adenine pair was used to indirectly determine thymine by SERS, improving the limit of detection values up to two orders of magnitude.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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