Fluorescent lactose-derived catanionic aggregates: synthesis, characterisation and potential use as antibacterial agents

RSC Advances ◽  
2016 ◽  
Vol 6 (28) ◽  
pp. 23340-23344
Author(s):  
Alexandre Bettoschi ◽  
Alain Brisson ◽  
Claudia Caltagirone ◽  
Angela M. Falchi ◽  
Francesco Isaia ◽  
...  
Keyword(s):  
Antibacterial Agents ◽  
Low Cost ◽  
Antibacterial Properties ◽  
Drug Resistant ◽  
Gram Positive ◽  
Organic Salts ◽  
Gram Positive Bacteria ◽  
Catanionic Surfactants ◽  
Potential Use

The antibacterial properties of catanionic surfactants based on fluorescent lactose-derivative organic salts using low-cost starting materials were investigated towards multi-drug-resistant Gram-positive bacteria.

Recent development of antibacterial agents to combat drug-resistant Gram-positive bacteria

2020 ◽  
pp. 71-104
Author(s):  
Mohini Mohan Konai ◽  
Swagatam Barman ◽  
Yash Acharya ◽  
Kathakali De ◽  
Jayanta Haldar
Keyword(s):  
Antibacterial Agents ◽  
Drug Resistant ◽  
Gram Positive ◽  
Gram Positive Bacteria

scholarly journals Antimicrobial activity of crude henna extract against Gram-positive bacteria

2021 ◽  
Vol 5 (3) ◽  
Keyword(s):  
Antimicrobial Activity ◽  
Crude Extract ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Drug Resistant ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Bacterial Agent ◽  
Henna Extract

Objectives: This study aimed to examine the effect of ethanolic extract of local Basra henna leaves on Gram-positive bacteria species. Also, to assess the antibacterial properties of henna crude extract in vitro and compare them with antibiotics. Methods: In this study, Lawsonia inermis (henna) leaves were extracted with ethanol using the solvent extraction technique. The pathogens were isolated from wound samples obtained from hospitalized patients in two different hospitals in Duhok city. The culture of thirty isolates had been recognized by routine methods. Different concentrations of ethanol crude extract were acquired and bio-assayed in vitro to inhibit the growth of five human pathogenic Gram-positive bacteria. Agar well diffusion assay was used for achieving henna antibiotic activity. Moreover, an antibiotics susceptibility test was done by the disk diffusion method using the Muller-Hinton agar medium. Results: The growth of all tested bacteria was suppressed to various degrees by increasing the concentration of the extract. The data has revealed that Staphylococcus aureus was more sensitive than other examined isolates, where the diameter zone of inhibition was ranging from 16-27, 14-25, and 8-18 mm for Staphylococcus epidermidis, Lactobacillus spp. and Streptococcus pneumonia respectively. The antimicrobial activity of henna extract indicates that it is suitable for being used as significant certain medications. Consequently, henna is active to serve as an anti-bacterial agent against multi-drug resistant Gram-positive bacteria. Conclusion: The antimicrobial activity of henna extract indicates that it is suitable for being used as significant certain medications. Consequently, henna is active to serve as an anti-bacterial agent against multi-drug resistant Gram-positive bacteria.

Preparation of bioactive and antibacterial PMMA-based bone cement by modification with quaternary ammonium and alkoxysilane

2021 ◽  
pp. 088532822110044
Author(s):  
Haiyang Wang ◽  
Toshinari Maeda ◽  
Toshiki Miyazaki
Keyword(s):  
Antibacterial Activity ◽  
Methyl Methacrylate ◽  
Bone Cement ◽  
Setting Time ◽  
Antibacterial Properties ◽  
The Body ◽  
Apatite Formation ◽  
Gram Positive ◽  
E Coli ◽  
Gram Positive Bacteria

Bone cement based on poly(methyl methacrylate) (PMMA) powder and methyl methacrylate (MMA) liquid is a very popular biomaterial used for the fixation of artificial joints. However, there is a risk of this cement loosening from bone because of a lack of bone-bonding bioactivity. Apatite formation in the body environment is a prerequisite for cement bioactivity. Additionally, suppression of infection during implantation is required for bone cements to be successfully introduced into the human body. In this study, we modified PMMA cement with γ-methacryloxypropyltrimetoxysilane and calcium acetate to introduce bioactive properties and 2-( tert-butylamino)ethyl methacrylate (TBAEMA) to provide antibacterial properties. The long-term antibacterial activity is attributed to the copolymerization of TBAEMA and MMA. As the TBAEMA content increased, the setting time increased and the compressive strength decreased. After soaking in simulated body fluid, an apatite layer was detected within 7 days, irrespective of the TBAEMA content. The cement showed better antibacterial activity against Gram-negative E. Coli than Gram-positive bacteria; however, of the Gram-positive bacteria investigated, B. subtilis was more susceptible than S. aureus.

DNA Binding Ligands with Excellent Antibiotic Potency Against Drug-Resistant Gram-Positive Bacteria.

ChemInform ◽  
2003 ◽  
Vol 34 (6) ◽  
Author(s):  
Roland W. Buerli ◽  
Yigong Ge ◽  
Sarah White ◽  
Eldon E. Baird ◽  
Sofia M. Touami ◽  
...  
Keyword(s):  
Dna Binding ◽  
Drug Resistant ◽  
Gram Positive ◽  
Gram Positive Bacteria

scholarly journals Mobilizable Rolling-Circle Replicating Plasmids from Gram-Positive Bacteria: A Low-Cost Conjugative Transfer

Plasmids ◽  
2015 ◽  
pp. 257-276
Author(s):  
Cris Fernández-López ◽  
Alicia Bravo ◽  
Sofía Ruiz-Cruz ◽  
Virtu Solano-Collado ◽  
Danielle A. Garsin ◽  
...  
Keyword(s):  
Low Cost ◽  
Conjugative Transfer ◽  
Gram Positive ◽  
Rolling Circle ◽  
Gram Positive Bacteria

scholarly journals Mobilizable Rolling-Circle Replicating Plasmids from Gram-Positive Bacteria: A Low-Cost Conjugative Transfer

2014 ◽  
Vol 2 (5) ◽  
Author(s):  
Cris Fernández-López ◽  
Alicia Bravo ◽  
Sofía Ruiz-Cruz ◽  
Virtu Solano-Collado ◽  
Danielle A. Garsin ◽  
...  
Keyword(s):  
Low Cost ◽  
Conjugative Transfer ◽  
Gram Positive ◽  
Rolling Circle ◽  
Gram Positive Bacteria

DNA binding ligands targeting drug-resistant Gram-positive bacteria. Part 1: Internal benzimidazole derivatives

2004 ◽  
Vol 14 (5) ◽  
pp. 1253-1257 ◽  
Author(s):  
Roland W. Bürli ◽  
Dustin McMinn ◽  
Jacob A. Kaizerman ◽  
Wenhao Hu ◽  
Yigong Ge ◽  
...  
Keyword(s):  
Dna Binding ◽  
Benzimidazole Derivatives ◽  
Drug Resistant ◽  
Gram Positive ◽  
Gram Positive Bacteria

scholarly journals Antibacterial Properties of Some Cyclic Organobismuth(III) Compounds

2005 ◽  
Vol 49 (7) ◽  
pp. 2729-2734 ◽  
Author(s):  
Toshiaki Kotani ◽  
Daisuke Nagai ◽  
Kensuke Asahi ◽  
Hitomi Suzuki ◽  
Fumiaki Yamao ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Properties ◽  
Cell Number ◽  
Viable Cell Number ◽  
Electron Microscopic ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Ring Compounds ◽  
Medical Disorders

ABSTRACT Bismuth compounds are known for their low levels of toxicity in mammals, and various types of bismuth salts have been used to treat medical disorders. As part of our program to probe this aspect of bismuth chemistry, cyclic organobismuth compounds 1 to 8 bearing a nitrogen or sulfur atom as an additional ring member have been synthesized, and their antimicrobial activities against five standard strains of gram-negative and gram-positive bacteria were assessed. The eight-membered-ring compounds, compounds 1 to 3, exhibited MICs of less than 0.5 μg/ml against Staphylococcus aureus and were more active than the six-membered ones, compounds 5 to 8 (MICs, 4.0 to 16 μg/ml). The gram-positive bacteria (Staphylococcus aureus, Bacillus subtilis, and Enterococcus faecalis) were more susceptible to both types of ring compounds than the gram-negative ones (Escherichia coli and Pseudomonas aeruginosa). Treatment with polymyxin B nonapeptide increased the susceptibility of E. coli to cyclic organobismuth compounds, indicating the low permeability of the outer membrane of gram-negative bacteria to the compounds. Compound 1 also had activity against methicillin-resistant S. aureus, which had an MIC for 90% of the hospital stock strains of 1.25 μg/ml. The killing curves for S. aureus treated with compound 1 or 3 revealed a static effect at a low dose (2× the MIC). However, when S. aureus was treated with 10× the MIC of compound 1 or 3, there was an approximately 3-log reduction in the viable cell number after 48 h of treatment. Electron microscopic inspection demonstrated a considerable increase in the size of S. aureus and the proportion of cells undergoing cell division after treatment with compound 1 at 0.5× the MIC.

scholarly journals A3, a Scorpion Venom Derived Peptide Analogue with Potent Antimicrobial and Antibiofilm Activity against Clinical Isolates of Multi-Drug Resistant Gram Positive Bacteria

2018 ◽  
Author(s):  
Ammar Almaaytah ◽  
Ahmad Farajallah ◽  
Ahmad Abualhaijaa ◽  
Qosay Al-balas
Keyword(s):  
Host Defense ◽  
Mammalian Cells ◽  
Scorpion Venom ◽  
Clinical Isolates ◽  
Drug Resistant ◽  
Gram Positive ◽  
Peptide Analogue ◽  
Gram Positive Bacteria ◽  
Conventional Antibiotics

Current research in the field of antimicrobials is focused on the development of novel antibiotics and antimicrobial agents to counteract the huge dilemma that the human population is mainly facing in regards to the rise of bacterial resistance and biofilm infections. Host Defense peptides (HDPs) are a promising group of molecules for antimicrobial development as they share unique characteristics suitable for antimicrobial activity including their broad spectrum of activity and potency against bacteria. AamAP1 is a novel HDP that was identified through molecular cloning from the venom of the North African scorpion Androctonus amoeruxi. In vitro antimicrobial assays revealed that the peptide displays moderate activity against different strains of Gram-positive and Gram-negative bacteria. Additionally, the peptide proved to be highly hemolytic and displaying significantly high toxicity against mammalian cells. In our study, a novel synthetic peptide analogue named A3 was designed from the naturally occurring scorpion venom host defense peptide. The design strategy depended on modifying the amino acid sequence of the parent peptide in order to increase its net positive charge, percentage helicity and optimize other physico-chemical parameters involved theoretically in HDPs activity. Accordingly, A3 was evaluated for its in vitro antimicrobial and anti-biofilm activity individually and in combination with four different types of conventional antibiotics against clinical isolates of multi-drug resistant (MDR) Gram-positive bacteria. A3 was also evaluated for its cytotoxicity against mammalian cells. A3 displayed potent and selective in vitro antimicrobial activities against a wide range of MDR Gram-positive bacteria. Our results also showed that combining A3 with conventional antibiotics displayed a synergistic mode of action which resulted in decreasing the MIC value for A3 peptide as low as 0.125 µM. These effective concentrations were associated with negligible toxicities on mammalian cells. In conclusion, A3 exhibits enhanced activity and selectivity when compared with the parent natural scorpion venom peptide. The combination of A3 with conventional antibiotics may be pursued as a potential novel treatment strategy against MDR and biofilm forming bacteria.

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