scholarly journals A HER2 selective theranostic agent for surgical resection guidance and photodynamic therapy

2016 ◽  
Vol 15 (10) ◽  
pp. 1227-1238 ◽  
Author(s):  
H. Pye ◽  
M. A. Butt ◽  
H. W. Reinert ◽  
A. Maruani ◽  
J. P. M. Nunes ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Surgical Resection ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
Fab Fragment ◽  
Theranostic Agent

This work describes production of a HER2 targeting antibody Fab fragment dual conjugated for both near-infrared fluorescent imaging and photodynamic therapy.

Lysosomal targeted NIR photosensitizer for photodynamic therapy and two-photon fluorescent imaging

2021 ◽  
Author(s):  
Ruiyuan Liu ◽  
Yuping Zhou ◽  
Di Zhang ◽  
Genghan He ◽  
Chuang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
High Fidelity ◽  
Design And Synthesis ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Design and synthesis of near-infrared (NIR) emissive fluorophore for imaging of organelle and photodynamic therapy has received enormous attention. Hence, NIR emissive fluorophore of high-fidelity lysosome targeting, two-photon fluorescence imaging,...

Folic Acid Functionalized Chlorin e6-Superparamagnetic Iron Oxide Nanocarriers as a Theranostic Agent for MRI-Guided Photodynamic Therapy

2021 ◽  
Vol 17 (2) ◽  
pp. 205-215
Author(s):  
Zhenbo Sun ◽  
Mingfang Luo ◽  
Jia Li ◽  
Ailing Wang ◽  
Xucheng Sun ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Folic Acid ◽  
Iron Oxide ◽  
Near Infrared ◽  
Biological Fluids ◽  
Superparamagnetic Iron Oxide ◽  
Chlorin E6 ◽  
Theranostic Agent

Imaging-guided cancer theranostic is a promising strategy for cancer diagnostic and therapeutic. Photodynamic therapy (PDT), as an approved treatment modality, is limited by the poor solubility and dispersion of photosensitizers (PS) in biological fluids. Herein, it is demonstrated that superparamagnetic iron oxide (SPIO)-based nanoparticles (SCFs), prepared by conjugated with Chlorin e6 (Ce6) and modified with folic acid (FA) on the surface, can be used as versatile drug delivery vehicles for effective PDT. The nanoparticles are great carriers for photosensitizer Ce6 with an extremely high loading efficiency. In vitro fluorescence imaging and in vivo magnetic resonance imaging (MRI) results indicated that SCFs selectively accumulated in tumor cells. Under near-infrared laser irradiation, SCFs were confirmed to be capable of inducing low cell viability of RM-1 cells In vitro and displaying efficient tumor ablation with negligible side effects in tumor-bearing mice models.

Abstract WP441: Photodynamic Therapy Using Protease-Mediated Theranostic Photodynamic Agent Reduces Cathepsin-B Activity in Mouse Carotid Atheromata In Vivo

Stroke ◽  
2013 ◽  
Vol 44 (suppl_1) ◽  
Author(s):  
Soo-Min Shon ◽  
Yongdoo Choi ◽  
Dong Kun Lee ◽  
Jeong-Yeon Kim ◽  
Jin-Yong Park ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Cathepsin B ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Continuous Wave ◽  
Knock Out ◽  
Theranostic Agent ◽  
Nirf Imaging ◽  
Bilateral Carotid

Background Carotid atherosclerosis is a major cause of ischemic stroke. Nanotechology-based theranostics for atherosclerosis and stroke is still in its infancy. Objective —To investigate if an intravenously-injected cathepsin-B activatable theranostic agent (L-SR15) would be cleaved in and release a fluorescent agent (chlorine-e6) in mouse carotid atheromata, allowing both the diagnostic visualization and therapeutic application of these fluorophores as photosensitizers during photodynamic therapy (PDT) to attenuate plaque-destabilizing cathepsin-B activity by selectively eliminating macrophages. Methods and Results Thirty-week-old Apolipoprotein-E knock-out mice received intravenous injection of L-SR15 theranostic agent (n=5), control agent D-SR16 (n=5), or PBS (n=5) three times at days 0, 7, and 14. Twenty-four hours after each injection, the bilateral carotid arteries were exposed and Cy5.5 near-infrared fluorescent (NIRF) imaging was performed. Fluorescent signal progressively accumulated in atheromata of the L-SR15 group animals only, indicating that photosensitizers had been released from the theranostic agent, and were accumulating in the plaque. After each imaging session, PDT was applied with a continuous-wave diode-laser. Additional Cy7 near infrared imaging with a CatB-sensing activatable NIRF imaging agent reduced CatB-realated signal only in the L-SR15, with activity at day 22 significantly lower than at baseline. Histological studies demonstrated that L-SR15-based PDT eliminated macrophages by inducing apoptosis without affecting plaque size or smooth muscle cell numbers. Conclusion This is the first study showing that macrophage-secreted cathepsin-B activity could be attenuated by PDT using a protease-mediated theranostic agent.

scholarly journals NIR Photosensitizer for Two-Photon Fluorescent Imaging and Photodynamic Therapy of Tumor

2021 ◽  
Vol 9 ◽  
Author(s):  
Lujia Chen ◽  
Meijuan Chen ◽  
Yuping Zhou ◽  
Changsheng Ye ◽  
Ruiyuan Liu
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
Deep Tissue ◽  
Two Photon ◽  
Nir Emission ◽  
Good Biocompatibility ◽  
Fluorescent Agent

Preparation of near-infrared (NIR) emissive fluorophore for imaging-guided PDT (photodynamic therapy) has attracted enormous attention. Hence, NIR photosensitizers of two-photon (TP) fluorescent imaging and photodynamic therapy are highly desirable. In this contribution, a novel D-π-A structured NIR photosensitizer (TTRE) is synthesized. TTRE demonstrates near-infrared (NIR) emission, good biocompatibility, and superior photostability, which can act as TP fluorescent agent for clear visualization of cells and vascular in tissue with deep-tissue penetration. The PDT efficacy of TTRE as photosensitizer is exploited in vitro and in vivo. All these results confirm that TTRE would serve as potential platform for TP fluorescence imaging and imaging-guided photodynamic therapy.

MC540 and Upconverting Nanocrystal Coloaded Polymeric Liposome for Near-Infrared Light-Triggered Photodynamic Therapy and Cell Fluorescent Imaging

2014 ◽  
Vol 6 (5) ◽  
pp. 3219-3225 ◽  
Author(s):  
Hanjie Wang ◽  
Zhongyun Liu ◽  
Sheng Wang ◽  
Chunhong Dong ◽  
Xiaoqun Gong ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Near Infrared Light

Construction of a near-infrared responsive upconversion nanoplatform against hypoxic tumors via NO-enhanced photodynamic therapy

Nanoscale ◽  
2020 ◽  
Vol 12 (14) ◽  
pp. 7875-7887 ◽  
Author(s):  
Ying Lan ◽  
Xiaohui Zhu ◽  
Ming Tang ◽  
Yihan Wu ◽  
Jing Zhang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Near Infrared ◽  
Upconversion Nanoparticles

A near-infrared (NIR) activated theranostic nanoplatform based on upconversion nanoparticles (UCNPs) is developed in order to overcome the hypoxia-associated resistance in photodynamic therapy by photo-release of NO upon NIR illumination.

A nitroreductase-activatable near-infrared theranostic photosensitizer for photodynamic therapy under mild hypoxia

2020 ◽  
Vol 56 (43) ◽  
pp. 5819-5822
Author(s):  
Jing Zheng ◽  
Yongzhuo Liu ◽  
Fengling Song ◽  
Long Jiao ◽  
Yingnan Wu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Triplet State ◽  
Near Infrared ◽  
Mild Hypoxia

In this study, a near-infrared (NIR) theranostic photosensitizer was developed based on a heptamethine aminocyanine dye with a long-lived triplet state.

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

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