Investigation of uranium interactions with calcium phosphate-binding proteins using ICP/MS and CE-ICP/MS

Metallomics ◽  
2016 ◽  
Vol 8 (11) ◽  
pp. 1185-1192 ◽  
Author(s):  
Thi-Ngoc Suong Huynh ◽  
Claude Vidaud ◽  
Agnès Hagège
Keyword(s):  
Calcium Phosphate ◽  
Binding Proteins ◽  
Phosphate Binding ◽  
Icp Ms

scholarly journals Phosphate removal and recovery using immobilized phosphate binding proteins

2018 ◽  
Vol 1 ◽  
pp. 100003 ◽  
Author(s):  
Kaushik Venkiteshwaran ◽  
Nilisha Pokhrel ◽  
Faten Hussein ◽  
Edwin Antony ◽  
Brooke K. Mayer
Keyword(s):  
Binding Proteins ◽  
Phosphate Removal ◽  
Phosphate Binding ◽  
Removal And Recovery

Polyester fibers can be rendered calcium phosphate-binding by surface functionalization with bisphosphonate groups

2017 ◽  
Vol 105 (8) ◽  
pp. 2335-2342 ◽  
Author(s):  
Alessandro Polini ◽  
Daniela Geta Petre ◽  
Michele Iafisco ◽  
Sonia de Lacerda Schickert ◽  
Anna Tampieri ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Surface Functionalization ◽  
Phosphate Binding ◽  
Polyester Fibers

scholarly journals Enhanced resistance inTheobroma cacaoagainst oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteins

2015 ◽  
Vol 14 (3) ◽  
pp. 875-886 ◽  
Author(s):  
Emily E. Helliwell ◽  
Julio Vega-Arreguín ◽  
Zi Shi ◽  
Bryan Bailey ◽  
Shunyuan Xiao ◽  
...  
Keyword(s):  
Fungal Pathogens ◽  
Binding Proteins ◽  
Phosphate Binding ◽  
Enhanced Resistance ◽  
Phosphatidylinositol 3

scholarly journals The Role of First and Second Shell Interactions in Phosphate Binding Proteins

2013 ◽  
Vol 104 (2) ◽  
pp. 405a ◽  
Author(s):  
Mathias Gruber ◽  
Caroline Junker ◽  
Per Greisen ◽  
Claus Hélix-Nielsen
Keyword(s):  
Binding Proteins ◽  
Phosphate Binding

Synthesis of calcium phosphate-binding liposome for drug delivery

2009 ◽  
Vol 19 (15) ◽  
pp. 4148-4150 ◽  
Author(s):  
Takahisa Anada ◽  
Yoichi Takeda ◽  
Yoshitomo Honda ◽  
Kazuo Sakurai ◽  
Osamu Suzuki
Keyword(s):  
Drug Delivery ◽  
Calcium Phosphate ◽  
Phosphate Binding

Immunoblot detection of pyridoxal phosphate binding proteins in liver and hepatoma cytosolic extracts

1983 ◽  
Vol 112 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Jason M. Kittler ◽  
Dace Viceps-Madore ◽  
John A. Cidlowski ◽  
John W. Thanassi
Keyword(s):  
Binding Proteins ◽  
Pyridoxal Phosphate ◽  
Phosphate Binding

scholarly journals Protein-Bound Calcium Phosphate in Uremic Rat Serum: A Quantitative Study

2020 ◽  
Author(s):  
Hong-Xing Fan ◽  
Bao-Di Gou ◽  
Yu-Xi Gao ◽  
Gang Wu ◽  
Shu-Hu Liu ◽  
...  
Keyword(s):  
Chronic Kidney Disease ◽  
Calcium Phosphate ◽  
Kidney Disease ◽  
Serum Proteins ◽  
Chemical Interaction ◽  
Molar Ratio ◽  
Phosphate Binding ◽  
Rat Serum ◽  
Discrete State ◽  
Edge Structure

Protein-bound calcium (prCa) constitutes about 40% of serum total calcium, in which albumin is the most dominant protein. Given the chemical interaction between calcium and phosphate (Pi), the increased serum Pi in chronic kidney disease may cause changes in the composition and structure of the prCa fraction. Here, we report <a>the phosphate binding on the protein-bound calcium in uremic rat serum.</a> Using adenine-fed rats as a uremic model, we determined the levels of calcium and phosphate fractions in rat serum by ultrafiltration, and found that the level of protein-bound phosphate (prPi) in the uremic serum was markedly higher than in control. The elevated prPi level was comparable to the prCa level, consistent with the presence of <a>protein-bound calcium phosphate</a> pr(Ca)<sub>j-m</sub>(CaPi)<sub>m</sub>. We then confirmed its presence by ex vivo X-ray absorption near-edge structure spectroscopy, revealing the discrete state of the calcium phosphate clusters associated with protein. Finally, in a quantitative investigation using Ca- and Pi-boosted serum, we discovered the threshold concentration for the Pi binding on prCa, and determined the binding constant. The threshold, while preventing Pi from binding to prCa in normal condition, allows excess Pi to do so. The protein-bound calcium phosphate could act as a link between the metabolism of serum proteins and the homeostasis of phosphate and calcium, and it deserves further investigation whether the molar ratio of (prPi/prCa)×100% may serve as a serum index of the vascular calcification status in chronic kidney disease.

Sign in / Sign up

Export Citation Format

Share Document