‘Light up’ protein–protein interaction through bioorthogonal incorporation of a turn-on fluorescent probe into β-lactamase

2016 ◽  
Vol 12 (12) ◽  
pp. 3544-3549 ◽  
Author(s):  
Rui Hu ◽  
Hong-Kin Yap ◽  
Yik-Hong Fung ◽  
Yong Wang ◽  
Wing-Lam Cheong ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Protein Interaction ◽  
Protein Protein Interaction ◽  
Turn On

Aggregation induced emissive compound EPB can detect protein–protein interaction.

A Fluorescent Probe for Imaging p53-MDM2 Protein-Protein Interaction

2014 ◽  
Vol 85 (4) ◽  
pp. 411-417 ◽  
Author(s):  
Zhenzhen Liu ◽  
Zhenyuan Miao ◽  
Jin Li ◽  
Kun Fang ◽  
Chunlin Zhuang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Protein Interaction ◽  
Mdm2 Protein ◽  
Protein Protein Interaction

scholarly journals Correction: Environment-sensitive turn-on fluorescent probes for p53–MDM2 protein–protein interaction

MedChemComm ◽  
2017 ◽  
Vol 8 (8) ◽  
pp. 1727-1727 ◽  
Author(s):  
Tingting Liu ◽  
Yan Jiang ◽  
Zhenzhen Liu ◽  
Jin Li ◽  
Kun Fang ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Fluorescent Probes ◽  
Mdm2 Protein ◽  
Protein Protein Interaction ◽  
Turn On

Correction for ‘Environment-sensitive turn-on fluorescent probes for p53–MDM2 protein–protein interaction’ by Tingting Liu et al., MedChemCommun, 2017, DOI: 10.1039/c7md00287d.

A Boronic Acid-Based Fluorescence Hydrogel for Monosaccharide Detection

2018 ◽  
Author(s):  
Suying Xu ◽  
Adam Sedgwick ◽  
Souad Elfecky ◽  
Wenbo Chen ◽  
Ashley Jones ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Boronic Acid ◽  
Binding Constants ◽  
Strong Fluorescence ◽  
Fluorescence Response ◽  
Turn On ◽  
Fluorescence Turn On ◽  
Hydrogel System

<p>A boronic acid-based anthracene fluorescent probe was functionalised with an acrylamide unit to incorporate into a hydrogel system for monosaccharide detection<i>. </i>In solution, the fluorescent probe<b> </b>displayed a strong fluorescence turn-on response upon exposure to fructose, and an expected trend in apparent binding constants, as judged by a fluorescence response where D-fructose > D-galactose > D-mannose > D-glucose. The hydrogel incorporating the boronic acid monomer demonstrated the ability to detect monosaccharides by fluorescence with the same overall trend as the monomer in solution with the addition of fructose resulting in a 10-fold enhancement (≤ 0.25 M). <b><u></u></b></p>

An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Yu-Miao Zhang ◽  
Jun Wang ◽  
Tao Wu
Keyword(s):  
Subcellular Localization ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Epidermal Cells ◽  
Cyclin Dependent Kinase ◽  
Protein Subcellular Localization ◽  
Protein Protein Interactions ◽  
Efficient System ◽  
Protein Protein Interaction ◽  
Onion Epidermal Cells

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.

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