Towards encoded particles for highly multiplexed colorimetric point of care autoantibody detection

Lab on a Chip ◽  
2017 ◽  
Vol 17 (3) ◽  
pp. 549-556 ◽  
Author(s):  
Gustav Svedberg ◽  
Yunjin Jeong ◽  
Hunjong Na ◽  
Jisung Jang ◽  
Peter Nilsson ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Encoded Particles ◽  
Autoantibody Detection

Multiplexed, scanner-based colorimetric detection of autoantibodies in plasma was achieved using graphically encoded particles and gold nanoparticles.

scholarly journals New portable smartphone-based PDMS microfluidic kit for the simultaneous colorimetric detection of arsenic and mercury

RSC Advances ◽  
2018 ◽  
Vol 8 (48) ◽  
pp. 27091-27100 ◽  
Author(s):  
Abbas Motalebizadeh ◽  
Hasan Bagheri ◽  
Sasan Asiaei ◽  
Nasim Fekrat ◽  
Abbas Afkhami
Keyword(s):  
Gold Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Microfluidic Platform

A smartphone-based microfluidic platform was developed for point-of-care (POC) detection using surface plasmon resonance (SPR) of gold nanoparticles (GNPs).

scholarly journals Development of label-free gold nanoparticle based rapid colorimetric assay for clinical/point-of-care screening of cervical cancer

2020 ◽  
Vol 2 (12) ◽  
pp. 5737-5745
Author(s):  
Tejaswini Appidi ◽  
Sushma V. Mudigunda ◽  
Suseela Kodandapani ◽  
Aravind Kumar Rengan
Keyword(s):  
Cervical Cancer ◽  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Assay ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Label Free ◽  
Free Gold ◽  
Clinical Point

“C-ColAur” technique for colorimetric detection of cervical cancer by in situ formation of gold nanoparticles.

scholarly journals Rapid Electronic Diagnostics of Ebola Virus with Synthetic Nanobody-Conjugated Gold Nanoparticles

2021 ◽  
Author(s):  
Xiahui Chen ◽  
Shoukai Kang ◽  
MD Ashif Ikbal ◽  
Zhi Zhao ◽  
Jiawei Zuo ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Infectious Diseases ◽  
Ebola Virus ◽  
Semiconductor Device ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Emerging Infectious Diseases ◽  
Rapid Diagnostics ◽  
Point Of Care Testing ◽  
Training Requirement

The success of controlling emerging infectious diseases relies on the fast development of robust, quantitative assays for point-of-care testing. Here a generalizable strategy is demonstrated for developing inexpensive, simple-to-use, and rapid diagnostics within a few weeks upon the identification of a new viral antigen. Using Ebola virus secreted glycoprotein (sGP) as a target, we design a new assay featuring nanobody-conjugated nanoparticles for rapid, electronic detection (Nano2RED). Nanobodies with the high affinity and specificity were generated by phage display screening of a high-quality combinatorial library (> 109) and site-specifically conjugated to gold nanoparticles (AuNPs) for in-solution colorimetric detection. Our assay can robustly detect the sGP protein from 10 pM to 100 nM in diluted serum and distinguish it from a membrane-anchored isoform, GP1,2, allowing the diagnosis of the viral infection stage. Additionally, a rapid assay protocol was established to decrease the assay time to a few minutes without compromising the accuracy. Lastly, this assay has been integrated with a portable semiconductor device with a digital readout and minimal training requirement for end users. Our method can be widely applied to the point-of-care testing of other infectious diseases.

DETECTION METHODS FOR RESULTS OF A LOOP-MEDIATED ISOTHERMAL AMPLIFICATION OF DNA

2020 ◽  
Vol 65 (1) ◽  
pp. 67-72
Author(s):  
Olga A. Petrusha ◽  
E. B. Faizuloev
Keyword(s):  
Gold Nanoparticles ◽  
Real Time ◽  
Fluorescence Detection ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lamp Reaction ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Advantages And Disadvantages ◽  
Point Of Care Diagnostics

The loop mediated isothermal amplification (LAMP) was developed by T. Notomi et al. in 2000. It has become one of the most promising methods for point-of-care diagnostics due to its accuracy, sensitivity and ease of execution. In this review, various methods for detecting the results of the LAMP reaction are considered; their advantages and disadvantages are revealed. Methods for detecting LAMP results can be divided into indirect and direct. Indirect methods aimed at detecting changes in the chemical composition of the reaction mixture include real-time turbidimetry, fluorescence detection with calcein, colorimetric detection with hydroxynaphthol blue, and detection using modified gold nanoparticles. Direct methods based on the detection of accumulation amplicons during the reaction include fluorimetric detection with intercalating dyes, resonance fluorescence energy transfer, enzyme immunoassay, immunochromatography, using cationic polymers and gold nanoparticles. The development in the field of point-of-care diagnostics is characterized by a pronounced tendency to miniaturization, the LAMP reaction on microchips and microfluidic devices with an electrochemical or optical detection method. The most promising for the diagnosis of infectious diseases are turbidimetry methods and the use of intercalating dyes. The development of portable domestic instruments for detecting of LAMP results based on real-time fluorescence detection or turbidimetry will contribute to the widespread introduction of the method into clinical laboratory diagnostic practice. A literature research was conducted in the Pubmed ncbi based on keywords.

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM).The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated at a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM). The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

scholarly journals CRISPR/Cas12a-mediated gold nanoparticle aggregation for colorimetric detection of SARS-CoV-2

2021 ◽  
Author(s):  
Yiren Cao ◽  
Jinjun Wu ◽  
Bo Pang ◽  
Hongquan Zhang ◽  
X. Chris Le
Keyword(s):  
Gold Nanoparticles ◽  
Gold Nanoparticle ◽  
Colorimetric Detection ◽  
Isothermal Amplification ◽  
Nanoparticle Aggregation ◽  
Cleavage Activity ◽  
Gold Nanoparticle Aggregation

The trans-cleavage activity of the target-activated CRISPR-Cas12a liberated an RNA crosslinker from a molecular transducer, which facilitated assembly of gold nanoparticles. Integration of the molecular transducer with isothermal amplification and...

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