scholarly journals Magnetic Nickel iron Electroformed Trap (MagNET): a master/replica fabrication strategy for ultra-high throughput (>100 mL h−1) immunomagnetic sorting

Lab on a Chip ◽  
2016 ◽  
Vol 16 (16) ◽  
pp. 3049-3057 ◽  
Author(s):  
Jina Ko ◽  
Venkata Yelleswarapu ◽  
Anup Singh ◽  
Nishal Shah ◽  
David Issadore
Keyword(s):  
High Throughput ◽  
Cell Separation ◽  
Specific Cell ◽  
New Approach ◽  
Nickel Iron ◽  
Immunomagnetic Sorting

We present a new approach to microfabricate micromagnetic sorting devices that can achieve highly specific cell separation at a throughput (ϕ> 150 mL h−1) 100× greater than conventional microfluidics.

Clog-free cell filtration using resettable cell traps

Lab on a Chip ◽  
2014 ◽  
Vol 14 (15) ◽  
pp. 2657-2665 ◽  
Author(s):  
William Beattie ◽  
Xi Qin ◽  
Lin Wang ◽  
Hongshen Ma
Keyword(s):  
High Throughput ◽  
Cell Separation ◽  
Free Cell ◽  
Separation Mechanism ◽  
Cell Filtration ◽  
Capture And Release

A microfluidic cell separation mechanism created using constrictions with adjustable size that can selectively capture and release cells, thereby enabling high throughput size and deformability based cell separation without clogging.

scholarly journals New Approach for High-Throughput Screening of Drug Activity on Plasmodium Liver Stages

2006 ◽  
Vol 50 (4) ◽  
pp. 1586-1589 ◽  
Author(s):  
Audrey Gego ◽  
Olivier Silvie ◽  
Jean-François Franetich ◽  
Khemaïs Farhati ◽  
Laurent Hannoun ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
High Throughput ◽  
High Throughput Screening ◽  
Scanning System ◽  
New Approach ◽  
Drug Activity ◽  
Hepatic Cells ◽  
High Throughput Drug Screening ◽  
Prophylactic Drugs

ABSTRACT Plasmodium liver stages represent potential targets for antimalarial prophylactic drugs. Nevertheless, there is a lack of molecules active on these stages. We have now developed a new approach for the high-throughput screening of drug activity on Plasmodium liver stages in vitro, based on an infrared fluorescence scanning system. This method allowed us to count automatically and rapidly Plasmodium-infected hepatocytes, using different hepatic cells and different Plasmodium species, including Plasmodium falciparum. This new technique is well adapted for high-throughput drug screening and should facilitate the identification of new antimalarial compounds active on Plasmodium liver stages.

Abstract 3480: High-throughput size and deformability-based cancer cell separation

2014 ◽  
Author(s):  
Gordon Yip ◽  
Daniel Ionescu ◽  
Edwin Johnson ◽  
Mikael Dick ◽  
Zecong Fang ◽  
...  
Keyword(s):  
Cancer Cell ◽  
High Throughput ◽  
Cell Separation

High-Throughput Screening of Chemical Libraries for Modulators of Mesenchymal Stem Cell Chondrogenesis

2008 ◽  
Author(s):  
Alice H. Huang ◽  
Nuzhat A. Motlekar ◽  
Ashley Stein ◽  
Eileen M. Shore ◽  
Scott L. Diamond ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Type Ii Collagen ◽  
Defined Medium ◽  
Cell Number ◽  
Specific Cell ◽  
High Throughput Analysis ◽  
Single Plate ◽  
Culture Duration ◽  
Cell Phenotypes

Mesenchymal stem cells (MSCs) are a multi-potential cell type that can be induced to differentiate to a variety of tissue-specific cell phenotypes, including cartilage (chondrogenesis) and bone (osteogenesis). Given this multi-potentiality, MSCs are a promising cell source for exploring developmental paradigms and for tissue engineering (TE) applications. For cartilage formation assays, MSCs are collected in high-density pellets and treated with specific biofactors, including TGF-β superfamily members and dexamethasone in a chemically defined medium (CM) [1]. During chondrogenesis, extracellular matrix (ECM) rich in glycosaminoglycan (GAG) and type II collagen is synthesized. While MSC chondrogenesis is well-characterized using existing protocols, the effect of alternative biofactors, their doses and combinations requires laborious combinatorial studies [2]. High-throughput screening (HTS) overcomes this limitation through the simultaneous layout and query of a large number of conditions within a single plate. HTS depends on the use of precise robotic liquid handling systems and on the development of sensitive, validated, and readily quantifiable assays. In a recent study, we optimized cell culture and assay procedures for HTS by minimizing cell number, handling and culture duration [3]. We successfully reduced the time scale from 21 to 7 days and the number of cells required from 225K to 30K cells per pellet. Further, we developed a novel in-well digestion protocol to enable high-throughput analysis and minimize handling. In this study, we have further streamlined these assays for HTS by providing a rapid and robotic approach for layout, culture, and analysis of ECM deposition using ‘micro’ MSC pellets (10K cells per pellet) in a 384-well format. Furthermore, we have carried out an initial screen of the NINDS small molecule library and demonstrated the feasibility of this technology for use in HTS of chondrogenesis.

scholarly journals Importance of high-throughput cell separation technologies for genomics/proteomics-based clinical diagnostics

2002 ◽  
Author(s):  
James F. Leary ◽  
Peter Szaniszlo ◽  
Tarl W. Prow ◽  
Lisa M. Reece ◽  
Nan Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Cell Separation ◽  
Clinical Diagnostics

Methods for advanced hepatocyte cell culture in microwells utilizing air bubbles

Lab on a Chip ◽  
2015 ◽  
Vol 15 (4) ◽  
pp. 1032-1037 ◽  
Author(s):  
Vasiliy N. Goral ◽  
Sam H. Au ◽  
Ronald A. Faris ◽  
Po Ki Yuen
Keyword(s):  
Cell Culture ◽  
High Throughput ◽  
Drug Screening ◽  
Specific Cell ◽  
Air Bubbles ◽  
Cytotoxicity Assays ◽  
High Throughput Drug Screening ◽  
Cellular Microenvironments ◽  
Substantial Control

We present a number of novel and unique methods for advanced cell culture in microwells utilizing air bubbles to provide substantial control over cellular microenvironments and organization in order to achieve specific cell-based applications, and an easy way for high throughput drug screening and cytotoxicity assays.

scholarly journals Automated, high-throughput immunofluorescence staining: a new approach

2007 ◽  
Vol 120 (1) ◽  
pp. 91-96 ◽  
Author(s):  
J. L. J. van de Bilt ◽  
J. H. J. Derks ◽  
J. D. Janse
Keyword(s):  
High Throughput ◽  
Immunofluorescence Staining ◽  
New Approach

Pinched flow coupled shear-modulated inertial microfluidics for high-throughput rare blood cell separation

Lab on a Chip ◽  
2011 ◽  
Vol 11 (11) ◽  
pp. 1870 ◽  
Author(s):  
Ali Asgar S. Bhagat ◽  
Han Wei Hou ◽  
Leon D. Li ◽  
Chwee Teck Lim ◽  
Jongyoon Han
Keyword(s):  
Blood Cell ◽  
High Throughput ◽  
Cell Separation ◽  
Inertial Microfluidics

Acoustic focusing with engineered node locations for high-performance microfluidic particle separation

The Analyst ◽  
2014 ◽  
Vol 139 (5) ◽  
pp. 1192-1200 ◽  
Author(s):  
Erika J. Fong ◽  
Amanda C. Johnston ◽  
Timothy Notton ◽  
Seung-Yong Jung ◽  
Klint A. Rose ◽  
...  
Keyword(s):  
High Throughput ◽  
High Performance ◽  
High Efficiency ◽  
Particle Separation ◽  
Main Channel ◽  
Thin Wall ◽  
Device Design ◽  
Secondary Channel ◽  
New Approach ◽  
Acoustic Focusing

We present a new approach to acoustofluidic device design with a secondary channel separated from the main channel by a thin wall. This allows off-center placement of acoustic nodes, which enables high-efficiency and high-throughput separation of cell-scale objects.

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