scholarly journals Gd3+–Gd3+ distances exceeding 3 nm determined by very high frequency continuous wave electron paramagnetic resonance

2017 ◽  
Vol 19 (7) ◽  
pp. 5127-5136 ◽  
Author(s):  
Jessica A. Clayton ◽  
Mian Qi ◽  
Adelheid Godt ◽  
Daniella Goldfarb ◽  
Songi Han ◽  
...  
Keyword(s):  
Continuous Wave ◽  
Very High Frequency ◽  
Fundamental Study ◽  
Paramagnetic Resonance ◽  
High Frequencies ◽  
Biologically Relevant ◽  
Lineshape Analysis ◽  
Very High Frequencies ◽  
Electron Paramagnetic ◽  
Very High

We present a fundamental study of a CW EPR lineshape analysis-based approach to determining Gd–Gd distances at very high frequencies (240 GHz). The approach is demonstrated from cryogenic to biologically-relevant temperatures.

An ammeter for very high frequencies

1930 ◽  
Vol 68 (401) ◽  
pp. 556-559
Author(s):  
C.L. Fortescue ◽  
L.A. Moxon
Keyword(s):  
High Frequencies ◽  
Very High Frequencies ◽  
Very High

scholarly journals Idiotypic analysis of potential and available repertoires in the arsonate system.

1984 ◽  
Vol 160 (1) ◽  
pp. 1-11 ◽  
Author(s):  
M Slaoui ◽  
O Leo ◽  
J Marvel ◽  
M Moser ◽  
J Hiernaux ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
B Cells ◽  
Keyhole Limpet Hemocyanin ◽  
High Frequencies ◽  
Specific Subset ◽  
A Minor ◽  
Very High Frequencies ◽  
Very High ◽  
Limiting Dilution

We have shown that, by suitable idiotypic manipulation, BALB/c mice can express the major cross-reactive idiotype (CRI) of A/J mice in response to azophenylarsonate (Ars). In order to know if the CRIA idiotype is present in the potential repertoire of BALB/c before any intentional selection, we used polyclonal activation in vitro and limiting dilution analysis. The readout was done with two monoclonal anti-CRIA antibodies that recognize distinct idiotopes on a CRIA+ A/J germline-encoded monoclonal antibody. We studied the frequency of CRIA+ lipopolysaccharide (LPS)-reactive cells in the spleens of nonimmune and immune A/J mice and in the spleens of naive and manipulated (i.e., producing CRIA+ antibodies) BALB/c mice. A/J and BALB/c naive individuals presented very high frequencies of Ars-specific B cells while the frequency of CRIA+ B cells was only a minor subset (0.5%) of the total Ars-specific subset in the two strains. When A/J mice were immunized with Ars-keyhole limpet hemocyanin, a clear preferential expansion of the CRIA+ minor subset of A/J mice was observed (100x). No such enhancement was observed in BALB/c mice similarly treated. Manipulated BALB/c mice presented a higher frequency of CRIA+ anti-Ars B cells than naive or antigen-immunized BALB/c individuals.

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