Studies of cytochrome c-551 unfolding using fluorescence correlation spectroscopy and other biophysical techniques

2016 ◽  
Vol 18 (35) ◽  
pp. 24537-24548
Author(s):  
Pallabi Sil ◽  
Simanta Sarani Paul ◽  
Eva Di Silvio ◽  
Carlo Travaglini-Allocatelli ◽  
Krishnananda Chattopadhyay
Keyword(s):  
Cytochrome C ◽  
Fluorescence Correlation Spectroscopy ◽  
Hydrogen Exchange ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Biophysical Techniques ◽  
Spectroscopy Studies

Fluorescence correlation spectroscopy studies with a bacterial cytochrome c labeled at different positions complement NMR hydrogen exchange results.

scholarly journals Imaging fluorescence correlation spectroscopy studies of dye diffusion in self-assembled organic nanotubes

2016 ◽  
Vol 18 (25) ◽  
pp. 16766-16774 ◽  
Author(s):  
Hao Xu ◽  
Shinobu Nagasaka ◽  
Naohiro Kameta ◽  
Mitsutoshi Masuda ◽  
Takashi Ito ◽  
...  
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Dye Diffusion ◽  
Self Assembled ◽  
Spectroscopy Studies ◽  
Organic Nanotubes

Imaging fluorescence correlation spectroscopy is used to elucidate the rate and mechanism for diffusion of charged molecules within charged, self-assembled organic nanotubes.

Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag for Fluorescence Correlation Spectroscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles. Spectroscopic Behavior in Aqueous Media

2003 ◽  
Vol 68 (11) ◽  
pp. 2105-2119 ◽  
Author(s):  
Jana Humpolíčková ◽  
Karel Procházka ◽  
Martin Hof
Keyword(s):  
Fluorescence Correlation Spectroscopy ◽  
Polymeric Micelles ◽  
Aqueous Media ◽  
Correlation Spectroscopy ◽  
Water Soluble ◽  
Time Resolved ◽  
Fluorescence Correlation ◽  
Block Copolymer Micelles ◽  
Spectroscopy Studies ◽  
Fluorescent Tag

Fluorescence behavior of octadecylrhodamine B (ORB) and the association-dependent changes in UV-VIS absorption and fluorescence spectra in aqueous solutions were studied by UV-VIS absorption spectroscopy and steady-state and time-resolved fluorometry. Spectral changes are interpreted in terms of the hydrophobically driven formation of H- and J-dimers and higher aggregates. The knowledge gained serves as a basis for the interpretation of results of the fluorescence correlation spectroscopy study of polymeric micelles labeled with ORB.

scholarly journals A simulation-guided fluorescence correlation spectroscopy tool to investigate the protonation dynamics of cytochrome c oxidase

2016 ◽  
Vol 18 (18) ◽  
pp. 12877-12885 ◽  
Author(s):  
A. Wolf ◽  
C. Schneider ◽  
T.-Y. Kim ◽  
K. Kirchberg ◽  
P. Volz ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Single Molecule ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
Fluorescence Correlation ◽  
Rate Dependent ◽  
Confocal Volume ◽  
Dependent Processes

Fluorescence correlation spectroscopy (FCS) is a single molecule based technique to temporally resolve rate-dependent processes by correlating the fluorescence fluctuations of individual molecules traversing through a confocal volume.

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