Selectively lighting up two-photon photodynamic activity in mitochondria with AIE-active iridium(iii) complexes

2017 ◽  
Vol 53 (12) ◽  
pp. 2052-2055 ◽  
Author(s):  
Jiangping Liu ◽  
Chengzhi Jin ◽  
Bo Yuan ◽  
Xingguo Liu ◽  
Yu Chen ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Photon Irradiation ◽  
Two Photon ◽  
Photodynamic Activity

Three AIE-active Ir(iii) complexes that preferentially accumulate in the mitochondria of cancer cells through endocytosis were manifested in a lit up photodynamic activity in mitochondria with efficient lethality towards cancer cells and multicellular tumor spheroids under two-photon irradiation.

Half-Sandwich Ir(III) Complex of N1-Pyridyl-7-azaindole Exceeds Cytotoxicity of Cisplatin at Various Human Cancer Cells and 3D Multicellular Tumor Spheroids

2018 ◽  
Vol 37 (16) ◽  
pp. 2749-2759 ◽  
Author(s):  
Pavel Štarha ◽  
Zdeněk Trávníček ◽  
Hana Crlíková ◽  
Ján Vančo ◽  
Jana Kašpárková ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Human Cancer ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Human Cancer Cells ◽  
Half Sandwich

scholarly journals Synergic effect of doxorubicin release and two-photon irradiation of Mn2+-doped Prussian blue nanoparticles on cancer therapy

RSC Advances ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 2646-2649 ◽  
Author(s):  
Lamiaa M. A. Ali ◽  
Emna Mathlouthi ◽  
Maëlle Cahu ◽  
Saad Sene ◽  
Morgane Daurat ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Prussian Blue ◽  
Cancer Cells ◽  
High Efficiency ◽  
Synergic Effect ◽  
Photon Irradiation ◽  
Two Photon ◽  
Prussian Blue Nanoparticles ◽  
Doxorubicin Release

Mn2+-doped Prussian blue nanoparticles loaded with doxorubicin present high efficiency for combined photothermal and chemotherapy of cancer cells with a synergic effect under two-photon irradiation.

Generation of multicellular tumor spheroids of breast cancer cells: How to go three-dimensional

2013 ◽  
Vol 437 (1) ◽  
pp. 17-19 ◽  
Author(s):  
Anika Nagelkerke ◽  
Johan Bussink ◽  
Fred C.G.J. Sweep ◽  
Paul N. Span
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Three Dimensional ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids

scholarly journals Dissecting multi drug resistance in head and neck cancer cells using multicellular tumor spheroids

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Mohammad Azharuddin ◽  
Karin Roberg ◽  
Ashis Kumar Dhara ◽  
Mayur Vilas Jain ◽  
Padraig Darcy ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Head And Neck ◽  
Cancer Cells ◽  
Cell Lines ◽  
Treated Patient ◽  
Drug Uptake ◽  
Multi Drug Resistance ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids

AbstractOne of the hallmarks of cancers is their ability to develop resistance against therapeutic agents. Therefore, developing effective in vitro strategies to identify drug resistance remains of paramount importance for successful treatment. One of the ways cancer cells achieve drug resistance is through the expression of efflux pumps that actively pump drugs out of the cells. To date, several studies have investigated the potential of using 3-dimensional (3D) multicellular tumor spheroids (MCSs) to assess drug resistance; however, a unified system that uses MCSs to differentiate between multi drug resistance (MDR) and non-MDR cells does not yet exist. In the present report we describe MCSs obtained from post-diagnosed, pre-treated patient-derived (PTPD) cell lines from head and neck squamous cancer cells (HNSCC) that often develop resistance to therapy. We employed an integrated approach combining response to clinical drugs and screening cytotoxicity, monitoring real-time drug uptake, and assessing transporter activity using flow cytometry in the presence and absence of their respective specific inhibitors. The report shows a comparative response to MDR, drug efflux capability and reactive oxygen species (ROS) activity to assess the resistance profile of PTPD MCSs and two-dimensional (2D) monolayer cultures of the same set of cell lines. We show that MCSs provide a robust and reliable in vitro model to evaluate clinical relevance. Our proposed strategy can also be clinically applicable for profiling drug resistance in cancers with unknown resistance profiles, which consequently can indicate benefit from downstream therapy.

scholarly journals Expression of HYOU1 via reciprocal crosstalk between NSCLC cells and HUVECs control cancer progression and chemoresistance in multicellular tumor spheroids

2020 ◽  
Author(s):  
Minji Lee ◽  
Yeonhwa Song ◽  
Inhee Choi ◽  
Su-Yeon Lee ◽  
Sanghwa Kim ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Cancer Progression ◽  
Direct Interaction ◽  
Lung Cancer Cells ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids ◽  
Incidence And Mortality ◽  
Cancer Types ◽  
Cancer Spheroids

Abstract Background Among all cancer types, lung cancer ranks highest worldwide in terms of both incidence and mortality. The crosstalk between lung cancer cells and their tumor microenvironment (TME) has begun to emerge as the “Achilles heel” of the disease and thus constitutes an attractive target for anticancer therapy. We previously revealed that crosstalk between lung cancer cells and endothelial cells (ECs) induces chemoresistance in multicellular tumor spheroids (MCTSs). Methods In this study, we idientified that factors secreted in response to crosstalk between ECs and lung cancer cells play pivotal roles in the development of chemoresistance in lung cancer spheroids. Results We determined that the expression of hypoxia up-regulated protein 1 (HYOU1) in lung cancer spheroids was increased by factors secreted in response to crosstalk between ECs and lung cancer cells. Direct interaction between lung cancer cells and ECs also caused an elevation in the expression of HYOU1 in MCTSs. Inhibition of HYOU1 expression not only suppressed stemness and malignancy, but also facilitated apoptosis and chemosensitivity in lung cancer MCTSs. Inhibition of HYOU1 expression also significantly increased the expression of interferon signaling components in lung cancer cells. Moreover, the activation of the PI3K/AKT/mTOR pathway was involved in the HYOU1-induced aggression of lung cancer cells. Conclusion Taken together, our results identify HYOU1, which is induced in response to crosstalk between ECs and lung cancer cells within the TME, as a potential therapeutic target for combating the aggressive behavior of cancer cells.

Effects of sex- and glucocorticoid steroids on breast cancer cells grown as either multicellular tumor spheroids or monolayers

1996 ◽  
Vol 58 (1) ◽  
pp. 13-19 ◽  
Author(s):  
Elisabeth Müller-Holzner ◽  
Christian Marth ◽  
Alain G. Zeimet ◽  
Ferdinand Hofstädter ◽  
Günter Daxenbichler
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Multicellular Tumor Spheroids ◽  
Tumor Spheroids
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