Cross-triggered and cascaded recycling amplification for ultrasensitive electrochemical sensing of the mutant human p53 gene

2016 ◽  
Vol 52 (56) ◽  
pp. 8707-8710 ◽  
Author(s):  
Cuiyun Yang ◽  
Baoting Dou ◽  
Jianmei Yang ◽  
Ruo Yuan ◽  
Yun Xiang
Keyword(s):  
Human Serum ◽  
Electrochemical Detection ◽  
P53 Gene ◽  
Electrochemical Sensing ◽  
Mutant P53 ◽  
Highly Sensitive ◽  
Recycling Amplification

Cross-triggered and cascaded recycling amplification enables the highly sensitive electrochemical detection of the mutant p53 gene in human serum.

Cross-triggered and cascaded recycling amplification system for electrochemical detection of circulating microRNA in human serum

2021 ◽  
Author(s):  
Baoting Dou ◽  
Hui Zhou ◽  
Yajun Hong ◽  
Liming Zhao ◽  
Po Wang
Keyword(s):  
Nucleic Acid ◽  
Human Serum ◽  
Electrochemical Detection ◽  
Linear Range ◽  
Electrochemical Sensing ◽  
Circulating Microrna ◽  
Enzyme Cleavage ◽  
Amplification System ◽  
Recycling Amplification

A cross-triggered and cascaded recycling amplification system was developed for electrochemical sensing of microRNA 122 based on DNAzyme/multicomponent nucleic acid enzyme cleavage technique and dumbbell-shaped probe. The linear range and...

Ultrasensitive electrochemical detection of Hg2+based on an Hg2+-triggered exonuclease III-assisted target recycling strategy

The Analyst ◽  
2018 ◽  
Vol 143 (23) ◽  
pp. 5771-5778 ◽  
Author(s):  
Xiaolei Song ◽  
Yu Wang ◽  
Su Liu ◽  
Xue Zhang ◽  
Haiwang Wang ◽  
...  
Keyword(s):  
Electrochemical Detection ◽  
Electrochemical Method ◽  
Sensitive Detection ◽  
Mercury Ions ◽  
Exonuclease Iii ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Recycling Amplification ◽  
Highly Sensitive Detection

An isothermal electrochemical method for the highly sensitive detection of mercury ions (Hg2+) was established based on Hg2+-triggered exonuclease III-aided target recycling amplification.

Coupling strand extension/excision amplification with target recycling enables highly sensitive and aptamer-based label-free sensing of ATP in human serum

The Analyst ◽  
2020 ◽  
Vol 145 (2) ◽  
pp. 434-439 ◽  
Author(s):  
Lin Xu ◽  
Bingying Jiang ◽  
Wenjiao Zhou ◽  
Ruo Yuan ◽  
Yun Xiang
Keyword(s):  
Human Serum ◽  
Signal Enhancement ◽  
Label Free ◽  
Target Recycling ◽  
Highly Sensitive ◽  
Label Free Detection ◽  
Recycling Amplification

The integration of strand extension and excision recycling amplification leads to substantial signal enhancement for highly sensitive and label-free detection of ATP.

Detection of Glucose in Human Serum Based on Silicon Dot Probe

2020 ◽  
Vol 16 (6) ◽  
pp. 744-752
Author(s):  
Kuan Luo ◽  
Xinyu Jiang
Keyword(s):  
Quantum Dots ◽  
Blood Glucose ◽  
Human Serum ◽  
Organic Dyes ◽  
Limit Of Detection ◽  
Fasting Blood Glucose ◽  
Glucose Biosensor ◽  
Metal Nanoclusters ◽  
Glucose Levels ◽  
Highly Sensitive

Background: Diabetes Mellitus (DM) is a major public metabolic disease that influences 366 million people in the world in 2011, and this number is predicted to rise to 552 million in 2030. DM is clinically diagnosed by a fasting blood glucose that is equal or greater than 7 mM. Therefore, the development of effective glucose biosensor has attracted extensive attention worldwide. Fluorescence- based strategies have sparked tremendous interest due to their rapid response, facile operation, and excellent sensitivity. Many fluorescent compounds have been employed for precise analysis of glucose, including quantum dots, noble metal nanoclusters, up-converting nanoparticles, organic dyes, and composite fluorescent microspheres. Silicon dot as promising quantum dots materials have received extensive attention, owing to their distinct advantages such as biocompatibility, low toxicity and high photostability. Methods: MnO2 nanosheets on the Si nanoparticles (NPs) surface serve as a quencher. Si NPs fluorescence can make a recovery by the addition of H2O2, which can reduce MnO2 to Mn2+, and the glucose can thus be monitored based on the enzymatic conversion of glucose by glucose oxidase to generate H2O2. Therefore, the glucose concentration can be derived by recording the fluorescence recovery spectra of the Si NPs. Results: This probe enabled selective detection of glucose with a linear range of 1-100 μg/mL and a limit of detection of 0.98 μg/mL. Compared with the commercial glucometer, this method showed favorable results and convincing reliability. Conclusion: We have developed a novel method based on MnO2 -nanosheet-modified Si NPs for rapid monitoring of blood glucose levels. By combining the highly sensitive H2O2/MnO2 reaction with the excellent photostability of Si NPs, a highly sensitive, selective, and cost-efficient sensing approach for glucose detection has been designed and applied to monitor glucose levels in human serum with satisfactory results.

Highly sensitive and ratiometric luminescence sensing of heparin through templated cyanostilbene assemblies

The Analyst ◽  
2021 ◽  
Author(s):  
Shubhra Kanti Bhaumik ◽  
Supratim Banerjee
Keyword(s):  
Human Serum ◽  
Cluster Formation ◽  
Fluorometric Detection ◽  
Aqueous Buffer ◽  
Highly Sensitive

Heparin templated highly emissive cluster formation of di-cationic cyanostilbenes was utilized for the fluorometric detection of the polyanion in aqueous buffer, human serum and plasma.

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